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- PDB-1h6q: Translationally Controlled Tumor-associated Protein p23fyp from S... -

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Basic information

Entry
Database: PDB / ID: 1h6q
TitleTranslationally Controlled Tumor-associated Protein p23fyp from Schizosaccharomyces pombe
ComponentsTRANSLATIONALLY CONTROLLED TUMOR PROTEIN
KeywordsTUMOR-ASSOCIATED PROTEIN / FUNCTION UNKNOWN
Function / homology
Function and homology information


guanyl nucleotide exchange factor inhibitor activity / cytoplasmic translation / calcium ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Translationally controlled tumor protein (TCTP) domain signature 1. / Translationally controlled tumour protein, conserved site / Translationally controlled tumor protein (TCTP) domain signature 2. / Metal Binding Protein, Guanine Nucleotide Exchange Factor; Chain A / Translationally controlled tumour protein / Translationally controlled tumour protein (TCTP) domain / Translationally controlled tumour protein / Translationally controlled tumor protein (TCTP) domain profile. / Mss4/translationally controlled tumour-associated TCTP / Mss4-like superfamily ...Translationally controlled tumor protein (TCTP) domain signature 1. / Translationally controlled tumour protein, conserved site / Translationally controlled tumor protein (TCTP) domain signature 2. / Metal Binding Protein, Guanine Nucleotide Exchange Factor; Chain A / Translationally controlled tumour protein / Translationally controlled tumour protein (TCTP) domain / Translationally controlled tumour protein / Translationally controlled tumor protein (TCTP) domain profile. / Mss4/translationally controlled tumour-associated TCTP / Mss4-like superfamily / Metal Binding Protein, Guanine Nucleotide Exchange Factor; Chain A / Beta Complex / Mainly Beta
Similarity search - Domain/homology
Translationally-controlled tumor protein homolog
Similarity search - Component
Biological speciesSCHIZOSACCHAROMYCES POMBE (fission yeast)
MethodSOLUTION NMR / simulated annealing
Model type detailsMINIMIZED AVERAGE
AuthorsThaw, P. / Baxter, N.J. / Sedelnikova, S.E. / Price, C. / Waltho, J.P. / Craven, C.J.
CitationJournal: Nat. Struct. Biol. / Year: 2001
Title: Structure of TCTP reveals unexpected relationship with guanine nucleotide-free chaperones.
Authors: Thaw, P. / Baxter, N.J. / Hounslow, A.M. / Price, C. / Waltho, J.P. / Craven, C.J.
History
DepositionJun 20, 2001Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 9, 2001Provider: repository / Type: Initial release
Revision 1.1May 8, 2011Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Jan 17, 2018Group: Database references / Category: citation
Item: _citation.journal_abbrev / _citation.page_last / _citation.title
Revision 1.4May 15, 2024Group: Data collection / Database references / Other
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_nmr_software / pdbx_nmr_spectrometer
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_mr / _pdbx_nmr_software.name / _pdbx_nmr_spectrometer.model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: TRANSLATIONALLY CONTROLLED TUMOR PROTEIN


Theoretical massNumber of molelcules
Total (without water)19,0661
Polymers19,0661
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / 100LOWEST ENERGY
RepresentativeModel #1

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Components

#1: Protein TRANSLATIONALLY CONTROLLED TUMOR PROTEIN / TCTP / P23FYP


Mass: 19066.473 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) SCHIZOSACCHAROMYCES POMBE (fission yeast)
Plasmid: PET22B / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q10344

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experimentType: NOESY
NMR detailsText: CLOSEST STRUCTURE TO THE MEAN. THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY

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Sample preparation

Sample conditionsIonic strength: 250MM NACL / pH: 5.1 / Pressure: 1 atm / Temperature: 308 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker AVANCEBrukerAVANCE5001
Bruker AVANCEBrukerAVANCE6002
Bruker AVANCEBrukerAVANCE8003

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Processing

NMR software
NameVersionDeveloperClassification
X-PLOR3.1BRUNGERrefinement
Felixstructure solution
X-PLORstructure solution
RefinementMethod: simulated annealing / Software ordinal: 1 / Details: STANDARD SIMULATED ANNEALING PROTOCOL
NMR ensembleConformer selection criteria: LOWEST ENERGY / Conformers calculated total number: 100 / Conformers submitted total number: 1

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