+Open data
-Basic information
Entry | Database: PDB / ID: 1gd2 | ||||||
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Title | CRYSTAL STRUCTURE OF BZIP TRANSCRIPTION FACTOR PAP1 BOUND TO DNA | ||||||
Components |
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Keywords | transcription/DNA / basic leucine zipper / protein-DNA complex / transcription-DNA COMPLEX | ||||||
Function / homology | Function and homology information : / chromatin-protein adaptor activity / chromatin => GO:0000785 / DNA binding, bending / RNA polymerase II transcription regulator complex / cellular response to oxidative stress / DNA-binding transcription activator activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II ...: / chromatin-protein adaptor activity / chromatin => GO:0000785 / DNA binding, bending / RNA polymerase II transcription regulator complex / cellular response to oxidative stress / DNA-binding transcription activator activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Schizosaccharomyces pombe (fission yeast) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Fujii, Y. / Shimizu, T. / Toda, T. / Yanagida, M. / Hakoshima, T. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2000 Title: Structural basis for the diversity of DNA recognition by bZIP transcription factors. Authors: Fujii, Y. / Shimizu, T. / Toda, T. / Yanagida, M. / Hakoshima, T. #1: Journal: Acta Crystallogr.,Sect.D / Year: 1998 Title: Crystallographic characterization of Pap1-DNA complex Authors: Fujii, Y. / Ohira, T. / Kyogoku, Y. / Toda, T. / Yanagida, M. / Hakoshima, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1gd2.cif.gz | 119.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1gd2.ent.gz | 90.5 KB | Display | PDB format |
PDBx/mmJSON format | 1gd2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gd/1gd2 ftp://data.pdbj.org/pub/pdb/validation_reports/gd/1gd2 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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Details | The biological assmbly is a dimer constructed from chain E and F, chain G and H, and chain I and J. |
-Components
#1: DNA chain | Mass: 3975.611 Da / Num. of mol.: 4 / Source method: obtained synthetically #2: Protein | Mass: 8357.505 Da / Num. of mol.: 6 / Fragment: LEUCINE ZIPPER DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Schizosaccharomyces pombe (fission yeast) Plasmid: PET3A / Production host: Escherichia coli (E. coli) / References: UniProt: Q01663 #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.67 Å3/Da / Density % sol: 66.52 % | ||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 5.8 Details: PEG 6000, KCl, MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU FR-C / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: May 12, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→45.6 Å / Num. all: 127588 / Num. obs: 53501 / % possible obs: 83.4 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 1 / Redundancy: 2.4 % / Biso Wilson estimate: 27 Å2 / Rmerge(I) obs: 0.052 / Net I/σ(I): 10.3 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 1.7 % / Rmerge(I) obs: 0.244 / Num. unique all: 3856 / % possible all: 60.2 |
Reflection shell | *PLUS % possible obs: 60.2 % |
-Processing
Software |
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Refinement | Resolution: 2→20 Å / Cross valid method: THROUGHOUT / σ(F): 2 / σ(I): 1 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2→20 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | |||||||||||||||||||||
Refinement | *PLUS | |||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 42.8 Å2 | |||||||||||||||||||||
Refine LS restraints | *PLUS
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