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- PDB-1gbr: ORIENTATION OF PEPTIDE FRAGMENTS FROM SOS PROTEINS BOUND TO THE N... -

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Entry
Database: PDB / ID: 1gbr
TitleORIENTATION OF PEPTIDE FRAGMENTS FROM SOS PROTEINS BOUND TO THE N-TERMINAL SH3 DOMAIN OF GRB2 DETERMINED BY NMR SPECTROSCOPY
Components
  • GROWTH FACTOR RECEPTOR-BOUND PROTEIN 2GRB2
  • SOS-A PEPTIDE
KeywordsSIGNAL TRANSDUCTION PROTEIN
Function / homology
Function and homology information


guanyl-nucleotide exchange factor activity => GO:0005085 / Signalling to RAS / Activated NTRK2 signals through FRS2 and FRS3 / SHC1 events in ERBB4 signaling / GRB2 events in ERBB2 signaling / EGFR Transactivation by Gastrin / SHC-mediated cascade:FGFR3 / SHC-mediated cascade:FGFR4 / SHC-mediated cascade:FGFR1 / SHC-mediated cascade:FGFR2 ...guanyl-nucleotide exchange factor activity => GO:0005085 / Signalling to RAS / Activated NTRK2 signals through FRS2 and FRS3 / SHC1 events in ERBB4 signaling / GRB2 events in ERBB2 signaling / EGFR Transactivation by Gastrin / SHC-mediated cascade:FGFR3 / SHC-mediated cascade:FGFR4 / SHC-mediated cascade:FGFR1 / SHC-mediated cascade:FGFR2 / MET activates PI3K/AKT signaling / PI-3K cascade:FGFR3 / PI-3K cascade:FGFR4 / SHC-related events triggered by IGF1R / Signal regulatory protein family interactions / PI-3K cascade:FGFR1 / PI-3K cascade:FGFR2 / SOS-mediated signalling / : / FRS-mediated FGFR3 signaling / FRS-mediated FGFR4 signaling / MET activates RAS signaling / MET activates PTPN11 / Costimulation by the CD28 family / FRS-mediated FGFR1 signaling / FRS-mediated FGFR2 signaling / PI3K events in ERBB2 signaling / Tie2 Signaling / GRB2 events in EGFR signaling / SHC1 events in EGFR signaling / Interleukin-15 signaling / FLT3 Signaling / CD28 dependent Vav1 pathway / MET activates RAP1 and RAC1 / RHOU GTPase cycle / Signaling by CSF3 (G-CSF) / MET receptor recycling / NCAM signaling for neurite out-growth / Erythropoietin activates RAS / Regulation of KIT signaling / GAB1 signalosome / Role of LAT2/NTAL/LAB on calcium mobilization / PI3K Cascade / Negative regulation of FGFR3 signaling / Negative regulation of FGFR4 signaling / anatomical structure formation involved in morphogenesis / Downstream signal transduction / Negative regulation of FGFR1 signaling / Negative regulation of FGFR2 signaling / Spry regulation of FGF signaling / Insulin receptor signalling cascade / FCERI mediated MAPK activation / Negative regulation of MET activity / lymphocyte homeostasis / Signal attenuation / RHO GTPases Activate WASPs and WAVEs / NRAGE signals death through JNK / Signaling by SCF-KIT / EGFR downregulation / GRB2:SOS provides linkage to MAPK signaling for Integrins / guanyl-nucleotide exchange factor adaptor activity / FCERI mediated Ca+2 mobilization / Grb2-EGFR complex / RAF/MAP kinase cascade / Interleukin receptor SHC signaling / G alpha (12/13) signalling events / PIP3 activates AKT signaling / regulation of pro-B cell differentiation / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Regulation of signaling by CBL / Regulation of actin dynamics for phagocytic cup formation / Cargo recognition for clathrin-mediated endocytosis / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / branching involved in labyrinthine layer morphogenesis / Clathrin-mediated endocytosis / RET signaling / COP9 signalosome / neurotrophin TRKA receptor binding / vesicle membrane / regulation of T cell differentiation in thymus / transmembrane receptor protein tyrosine kinase adaptor activity / positive regulation of small GTPase mediated signal transduction / DAP12 signaling / epidermal growth factor receptor binding / small GTPase-mediated signal transduction / positive regulation of actin filament polymerization / regulation of T cell proliferation / ERBB2-ERBB3 signaling pathway / endodermal cell differentiation / regulation of MAPK cascade / B cell homeostasis / insulin receptor substrate binding / fibroblast growth factor receptor signaling pathway / signal transduction in response to DNA damage / Schwann cell development / myelination / phosphotyrosine residue binding / ephrin receptor binding / guanyl-nucleotide exchange factor activity / insulin-like growth factor receptor signaling pathway
Similarity search - Function
GRB2, N-terminal SH3 domain / GRB2, C-terminal SH3 domain / Grb2-like / Ras guanine-nucleotide exchange factor, conserved site / Ras Guanine-nucleotide exchange factors domain signature. / RasGEF N-terminal motif / Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif / Ras-like guanine nucleotide exchange factor, N-terminal / Ras guanine-nucleotide exchange factors N-terminal domain profile. / Ras-like guanine nucleotide exchange factor ...GRB2, N-terminal SH3 domain / GRB2, C-terminal SH3 domain / Grb2-like / Ras guanine-nucleotide exchange factor, conserved site / Ras Guanine-nucleotide exchange factors domain signature. / RasGEF N-terminal motif / Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif / Ras-like guanine nucleotide exchange factor, N-terminal / Ras guanine-nucleotide exchange factors N-terminal domain profile. / Ras-like guanine nucleotide exchange factor / Ras guanine-nucleotide exchange factor, catalytic domain superfamily / Ras guanine nucleotide exchange factor domain superfamily / RasGEF domain / Ras guanine-nucleotide exchange factors catalytic domain profile. / Guanine nucleotide exchange factor for Ras-like small GTPases / Ras guanine-nucleotide exchange factors catalytic domain / Dbl homology (DH) domain superfamily / RhoGEF domain / Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases / Dbl homology (DH) domain / Dbl homology (DH) domain profile. / SH3 Domains / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / SH3 domain / SH2 domain / Histone H2A/H2B/H3 / Core histone H2A/H2B/H3/H4 / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / SH3 type barrels. / Src homology 3 domains / SH2 domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Histone-fold / PH-like domain superfamily / Roll / Mainly Beta
Similarity search - Domain/homology
Son of sevenless homolog 2 / Growth factor receptor-bound protein 2
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodSOLUTION NMR
AuthorsWittekind, M. / Mapelli, C. / Farmer, B.T. / Suen, K.-L. / Goldfarb, V. / Tsao, J. / Lavoie, T. / Barbacid, M. / Meyers, C.A. / Mueller, L.
Citation
Journal: Biochemistry / Year: 1994
Title: Orientation of peptide fragments from Sos proteins bound to the N-terminal SH3 domain of Grb2 determined by NMR spectroscopy.
Authors: Wittekind, M. / Mapelli, C. / Farmer 2nd., B.T. / Suen, K.L. / Goldfarb, V. / Tsao, J. / Lavoie, T. / Barbacid, M. / Meyers, C.A. / Mueller, L.
#1: Journal: Mol.Cell.Biol. / Year: 1993
Title: Molecular Cloning of the Mouse Grb2 Gene: Differential Interaction of the Grb2 Adaptor Protein with Epidermal Growth Factor and Nerve Growth Factor Receptors
Authors: Suen, K.-L. / Bustelo, X.R. / Pawson, T. / Barbacid, M.
#2: Journal: Proc.Natl.Acad.Sci.USA / Year: 1992
Title: Identification of Murine Homologues of the Drosophila Son of Sevenless Gene: Potential Activators of Ras
Authors: Bowtell, D. / Fu, P. / Simon, M. / Senior, P.
History
DepositionAug 12, 1994Processing site: BNL
Revision 1.0Jan 26, 1995Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 23, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_ref_seq_dif.details
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

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Assembly

Deposited unit
A: GROWTH FACTOR RECEPTOR-BOUND PROTEIN 2
B: SOS-A PEPTIDE


Theoretical massNumber of molelcules
Total (without water)10,2262
Polymers10,2262
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)29 / -
Representative

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Components

#1: Protein GROWTH FACTOR RECEPTOR-BOUND PROTEIN 2 / GRB2


Mass: 8454.495 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: MOUSE GRB2 / Plasmid: PGTX-2T (PHARMACIA) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q60631
#2: Protein/peptide SOS-A PEPTIDE


Mass: 1771.150 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / References: UniProt: Q02384

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Processing

Software
NameClassification
X-PLORmodel building
X-PLORrefinement
X-PLORphasing
NMR softwareName: X-PLOR / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 29

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