- PDB-1g25: SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF THE HUMAN TFIIH MA... -
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基本情報
登録情報
データベース: PDB / ID: 1g25
タイトル
SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF THE HUMAN TFIIH MAT1 SUBUNIT
要素
CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1
キーワード
METAL BINDING PROTEIN / RING FINGER (C3HC4)
機能・相同性
機能・相同性情報
negative regulation of DNA helicase activity / ventricular system development / cyclin-dependent protein kinase activating kinase holoenzyme complex / CAK-ERCC2 complex / transcription factor TFIIK complex / adult heart development / transcription factor TFIIH holo complex / transcription factor TFIIH core complex / cyclin-dependent protein serine/threonine kinase activator activity / RNA Polymerase I Transcription Termination ...negative regulation of DNA helicase activity / ventricular system development / cyclin-dependent protein kinase activating kinase holoenzyme complex / CAK-ERCC2 complex / transcription factor TFIIK complex / adult heart development / transcription factor TFIIH holo complex / transcription factor TFIIH core complex / cyclin-dependent protein serine/threonine kinase activator activity / RNA Polymerase I Transcription Termination / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / regulation of G1/S transition of mitotic cell cycle / RNA Polymerase I Transcription Initiation / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / Formation of HIV elongation complex in the absence of HIV Tat / Cyclin E associated events during G1/S transition / RNA Polymerase II Transcription Elongation / Cyclin A/B1/B2 associated events during G2/M transition / Formation of RNA Pol II elongation complex / Cyclin A:Cdk2-associated events at S phase entry / RNA Polymerase II Pre-transcription Events / nucleotide-excision repair / RNA Polymerase I Promoter Escape / TP53 Regulates Transcription of DNA Repair Genes / positive regulation of smooth muscle cell proliferation / G1/S transition of mitotic cell cycle / NoRC negatively regulates rRNA expression / Transcription-Coupled Nucleotide Excision Repair (TC-NER) / Formation of TC-NER Pre-Incision Complex / Formation of Incision Complex in GG-NER / response to calcium ion / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / Cyclin D associated events in G1 / RUNX1 regulates transcription of genes involved in differentiation of HSCs / protein-containing complex assembly / transcription by RNA polymerase II / DNA修復 / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / zinc ion binding / 核質 類似検索 - 分子機能
Text: This structure was determined using standard 2D homonuclear techniques.
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試料調製
詳細
内容: 2 mM MAT1; 20 mM Tris-HCl pH 7.5; 90% H2O, 10% D2O / 溶媒系: 90% H2O/10% D2O
試料状態
Conditions-ID
pH
圧 (kPa)
温度 (K)
1
7.5
ambiant
298K
2
7.5
ambiant
290K
3
7.5
ambiant
283K
結晶化
*PLUS
手法: その他 / 詳細: NMR
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NMR測定
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M
放射波長
相対比: 1
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker DMX
Bruker
DMX
750
1
Bruker DRX
Bruker
DRX
600
2
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解析
NMR software
名称
バージョン
開発者
分類
X-PLOR
3.8
Brunger
構造決定
X-PLOR
3.8
Brunger
精密化
XwinNMR
1.3
Bruker
collection
XwinNMR
1.3
Bruker
解析
XEASY
1.3.13
Bartels
データ解析
精密化
手法: simulated annealing / ソフトェア番号: 1 詳細: the structures are based on a total of 988 restraints, 897 are NOE-derived distance constraints, 72 dihedral angle restraints,11 distance restraints from hydrogen bonds and 8 distance ...詳細: the structures are based on a total of 988 restraints, 897 are NOE-derived distance constraints, 72 dihedral angle restraints,11 distance restraints from hydrogen bonds and 8 distance restraints from the zinc binding.
代表構造
選択基準: fewest violations,lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the least restraint violations,structures with the lowest energy 計算したコンフォーマーの数: 57 / 登録したコンフォーマーの数: 20