+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1fq9 | ||||||||||||
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タイトル | CRYSTAL STRUCTURE OF A TERNARY FGF2-FGFR1-HEPARIN COMPLEX | ||||||||||||
要素 |
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キーワード | GROWTH FACTOR/GROWTH FACTOR RECEPTOR / I-set subgroup within the immunoglobulin superfamily / b-trefoil fold / GROWTH FACTOR-GROWTH FACTOR RECEPTOR COMPLEX | ||||||||||||
機能・相同性 | 機能・相同性情報 growth factor dependent regulation of skeletal muscle satellite cell proliferation / positive regulation of inner ear auditory receptor cell differentiation / positive regulation of neuroepithelial cell differentiation / regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis / positive regulation of lens fiber cell differentiation / positive regulation of cell fate specification / regulation of retinal cell programmed cell death / Formation of intermediate mesoderm / regulation of cell migration involved in sprouting angiogenesis / FGFRL1 modulation of FGFR1 signaling ...growth factor dependent regulation of skeletal muscle satellite cell proliferation / positive regulation of inner ear auditory receptor cell differentiation / positive regulation of neuroepithelial cell differentiation / regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis / positive regulation of lens fiber cell differentiation / positive regulation of cell fate specification / regulation of retinal cell programmed cell death / Formation of intermediate mesoderm / regulation of cell migration involved in sprouting angiogenesis / FGFRL1 modulation of FGFR1 signaling / corticotropin hormone secreting cell differentiation / thyroid-stimulating hormone-secreting cell differentiation / chondroblast differentiation / lymphatic endothelial cell migration / chemokine binding / negative regulation of fibroblast growth factor receptor signaling pathway / Signaling by FGFR1 amplification mutants / negative regulation of fibroblast growth factor production / positive regulation of mitotic cell cycle DNA replication / regulation of extrinsic apoptotic signaling pathway in absence of ligand / Signaling by plasma membrane FGFR1 fusions / diphosphate metabolic process / FGFR1c and Klotho ligand binding and activation / POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation / vitamin D3 metabolic process / regulation of phosphate transport / regulation of lateral mesodermal cell fate specification / positive regulation of cerebellar granule cell precursor proliferation / positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway / cementum mineralization / positive regulation of endothelial cell chemotaxis to fibroblast growth factor / cerebellar granule cell precursor proliferation / positive regulation of stem cell differentiation / response to sodium phosphate / hyaluronan catabolic process / regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling / receptor-receptor interaction / fibroblast growth factor receptor signaling pathway involved in orbitofrontal cortex development / auditory receptor cell development / ventricular zone neuroblast division / regulation of endothelial cell chemotaxis to fibroblast growth factor / Epithelial-Mesenchymal Transition (EMT) during gastrulation / glial cell differentiation / inner ear auditory receptor cell differentiation / negative regulation of wound healing / positive regulation of parathyroid hormone secretion / chordate embryonic development / stem cell development / Signaling by activated point mutants of FGFR3 / FGFR3c ligand binding and activation / Phospholipase C-mediated cascade; FGFR3 / mesenchymal cell proliferation / embryonic morphogenesis / FGFR2b ligand binding and activation / paraxial mesoderm development / fibroblast growth factor receptor binding / angiogenesis involved in coronary vascular morphogenesis / FGFR2c ligand binding and activation / Activated point mutants of FGFR2 / positive regulation of epithelial tube formation / Phospholipase C-mediated cascade; FGFR2 / FGFR4 ligand binding and activation / FGFR1b ligand binding and activation / fibroblast growth factor receptor activity / branching involved in salivary gland morphogenesis / mammary gland epithelial cell differentiation / Phospholipase C-mediated cascade; FGFR4 / Signaling by activated point mutants of FGFR1 / FGFR1c ligand binding and activation / organ induction / Downstream signaling of activated FGFR1 / Phospholipase C-mediated cascade: FGFR1 / positive regulation of phospholipase activity / lung-associated mesenchyme development / 傍分泌 / cell projection assembly / negative regulation of fibroblast migration / positive regulation of endothelial cell chemotaxis / phosphatidylinositol-mediated signaling / cell migration involved in sprouting angiogenesis / outer ear morphogenesis / middle ear morphogenesis / endothelial cell proliferation / embryonic limb morphogenesis / skeletal system morphogenesis / embryo development ending in birth or egg hatching / ureteric bud development / positive regulation of mesenchymal cell proliferation / cardiac muscle cell proliferation / positive regulation of vascular endothelial cell proliferation / Signaling by FGFR2 IIIa TM / inner ear morphogenesis / positive regulation of DNA biosynthetic process / Syndecan interactions / branching involved in ureteric bud morphogenesis / midbrain development / positive regulation of neuroblast proliferation / positive regulation of cell migration involved in sprouting angiogenesis / PI-3K cascade:FGFR3 / fibroblast growth factor binding 類似検索 - 分子機能 | ||||||||||||
生物種 | Homo sapiens (ヒト) | ||||||||||||
手法 | X線回折 / シンクロトロン / 解像度: 3 Å | ||||||||||||
データ登録者 | Schlessinger, J. / Plotnikov, A.N. / Ibrahimi, O.A. / Eliseenkova, A.V. / Yeh, B.K. / Yayon, A. / Linhardt, R.J. / Mohammadi, M. | ||||||||||||
引用 | ジャーナル: Mol.Cell / 年: 2000 タイトル: Crystal structure of a ternary FGF-FGFR-heparin complex reveals a dual role for heparin in FGFR binding and dimerization. 著者: Schlessinger, J. / Plotnikov, A.N. / Ibrahimi, O.A. / Eliseenkova, A.V. / Yeh, B.K. / Yayon, A. / Linhardt, R.J. / Mohammadi, M. | ||||||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1fq9.cif.gz | 143 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1fq9.ent.gz | 118.9 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1fq9.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/fq/1fq9 ftp://data.pdbj.org/pub/pdb/validation_reports/fq/1fq9 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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単位格子 |
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-要素
#1: タンパク質 | 分子量: 15110.339 Da / 分子数: 2 断片: THE B-TREFOIL CORE OF FIBROBLAST GROWTH FACTOR 2 (FGF2) 変異: C69S, C87S / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P09038 #2: タンパク質 | 分子量: 25274.805 Da / 分子数: 2 断片: EXTRACELLULAR LIGAND BINDING DOMAIN OF FGF RECEPTOR 1 (FGFR1) CONSISTING OF IMMUNOGLOBULIN LIKE DOMAINS II (D2) AND III (D3) 変異: N185Q / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P11362 #3: 多糖 | 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)- ...4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-beta-L-altropyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose | タイプ: oligosaccharideオリゴ糖 / 分子量: 2309.882 Da / 分子数: 1 / 由来タイプ: 組換発現 #4: 多糖 | 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)- ...4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose | |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.97 Å3/Da / 溶媒含有率: 58.57 % | ||||||||||||||||||||||||||||||||||||||||||
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結晶化 | 温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8.5 詳細: Ammonium sulfate, Glycerol, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K | ||||||||||||||||||||||||||||||||||||||||||
結晶 | *PLUS 溶媒含有率: 58 % | ||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 温度: 20 ℃ | ||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 110 K |
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放射光源 | 由来: シンクロトロン / サイト: NSLS / ビームライン: X4A / 波長: 0.9788 |
検出器 | タイプ: SDMS / 検出器: AREA DETECTOR / 日付: 1999年6月22日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.9788 Å / 相対比: 1 |
反射 | 解像度: 3→30 Å / Num. all: 97669 / Num. obs: 97669 / % possible obs: 97.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / 冗長度: 4.94 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 12.3 |
反射 シェル | 解像度: 3→3.11 Å / Rmerge(I) obs: 0.303 / % possible all: 92.6 |
反射 | *PLUS Num. obs: 19774 / Num. measured all: 97669 |
反射 シェル | *PLUS % possible obs: 92.6 % |
-解析
ソフトウェア |
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精密化 | 解像度: 3→25 Å / σ(F): 0 / σ(I): 0 / 立体化学のターゲット値: Engh & Huber
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精密化ステップ | サイクル: LAST / 解像度: 3→25 Å
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拘束条件 |
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