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Open data
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Basic information
Entry | Database: PDB / ID: 1fkx | ||||||
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Title | MURINE ADENOSINE DEAMINASE (D296A) | ||||||
![]() | ADENOSINE DEAMINASE![]() | ||||||
![]() | ![]() ![]() | ||||||
Function / homology | ![]() mature B cell apoptotic process / xanthine biosynthetic process / negative regulation of penile erection / Purine salvage / Ribavirin ADME / negative regulation of circadian sleep/wake cycle, non-REM sleep / negative regulation of mucus secretion / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Wilson, D.K. / Quiocho, F.A. | ||||||
![]() | ![]() Title: Probing the functional role of two conserved active site aspartates in mouse adenosine deaminase. Authors: Sideraki, V. / Mohamedali, K.A. / Wilson, D.K. / Chang, Z. / Kellems, R.E. / Quiocho, F.A. / Rudolph, F.B. #1: ![]() Title: A Pre-Transition-State Mimic of an Enzyme: X-Ray Structure of Adenosine Deaminase with Bound 1-Deazaadenosine and Zinc-Activated Water Authors: Wilson, D.K. / Quiocho, F.A. #2: ![]() Title: Atomic Structure of Adenosine Deaminase Complexed with a Transition-State Analog: Understanding Catalysis and Immunodeficiency Mutations Authors: Wilson, D.K. / Rudolph, F.B. / Quiocho, F.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 83.8 KB | Display | ![]() |
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PDB format | ![]() | 61.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 39671.180 Da / Num. of mol.: 1 / Mutation: D296A Source method: isolated from a genetically manipulated source Details: LIGANDED TO ZINC, 6(R)-HYDROXY-1,6-DIHYDROPURINE RIBOSIDE Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-PRH / |
#4: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.54 Å3/Da / Density % sol: 65.25 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | *PLUS pH: 4.2 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: May 2, 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Num. obs: 15662 / Observed criterion σ(I): 1 / Redundancy: 1.6 % / Rmerge(I) obs: 0.0422 |
Reflection | *PLUS Highest resolution: 2.4 Å / Num. measured all: 25228 / Rmerge(I) obs: 0.042 |
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Processing
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Refinement | Resolution: 2.4→8 Å / σ(F): 1 /
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Refinement step | Cycle: LAST / Resolution: 2.4→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.17 / Rfactor Rwork![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_deg / Dev ideal: 1.68 |