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Yorodumi- PDB-1fk1: STRUCTURAL BASIS OF NON-SPECIFIC LIPID BINDING IN MAIZE LIPID-TRA... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fk1 | ||||||
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Title | STRUCTURAL BASIS OF NON-SPECIFIC LIPID BINDING IN MAIZE LIPID-TRANSFER PROTEIN COMPLEXES WITH LAURIC ACID REVEALED BY HIGH-RESOLUTION X-RAY CRYSTALLOGRAPHY | ||||||
Components | NON-SPECIFIC LIPID TRANSFER PROTEIN | ||||||
Keywords | LIPID TRANSPORT / protein-lipid complex | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Zea mays (maize) | ||||||
Method | X-RAY DIFFRACTION / molecular replacement / Resolution: 1.8 Å | ||||||
Authors | Han, G.W. / Lee, J.Y. / Song, H.K. / Shin, D.H. / Suh, S.W. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2001 Title: Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes revealed by high-resolution X-ray crystallography. Authors: Han, G.W. / Lee, J.Y. / Song, H.K. / Chang, C. / Min, K. / Moon, J. / Shin, D.H. / Kopka, M.L. / Sawaya, M.R. / Yuan, H.S. / Kim, T.D. / Choe, J. / Lim, D. / Moon, H.J. / Suh, S.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fk1.cif.gz | 27.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fk1.ent.gz | 18.1 KB | Display | PDB format |
PDBx/mmJSON format | 1fk1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fk/1fk1 ftp://data.pdbj.org/pub/pdb/validation_reports/fk/1fk1 | HTTPS FTP |
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-Related structure data
Related structure data | 1fk0C 1fk2C 1fk3C 1fk4C 1fk5C 1fk6C 1fk7C 1mzlS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 9062.161 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: P19656 | ||
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#2: Chemical | ChemComp-DAO / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 48.02 % | |||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop Details: 4.2M Na formate, 0.2M potassium cyanide, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K | |||||||||||||||
Crystal grow | *PLUS Temperature: 24.5-25.5 ℃ | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: ENRAF-NONIUS / Detector: AREA DETECTOR / Date: Sep 5, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Highest resolution: 1.76 Å / Num. obs: 8622 / % possible obs: 91.4 % / Redundancy: 2.69 % / Rmerge(I) obs: 0.044 |
Reflection | *PLUS Num. obs: 8570 / Num. measured all: 23095 |
-Processing
Software |
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Refinement | Method to determine structure: molecular replacement Starting model: (PDB code:1MZL) Resolution: 1.8→8 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.8→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.843 / Classification: refinement | ||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 8 Å / σ(F): 2 / Rfactor obs: 0.195 / Rfactor Rfree: 0.211 | ||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||
Displacement parameters | *PLUS |