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- PDB-1fco: CRYSTAL STRUCTURE OF THE E. COLI AMPC BETA-LACTAMASE COVALENTLY A... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1fco | ||||||
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Title | CRYSTAL STRUCTURE OF THE E. COLI AMPC BETA-LACTAMASE COVALENTLY ACYLATED WITH THE INHIBITORY BETA-LACTAM, MOXALACTAM | ||||||
![]() | BETA-LACTAMASE![]() | ||||||
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Function / homology | ![]() antibiotic catabolic process / ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Patera, A. / Blaszczak, L.C. / Shoichet, B.K. | ||||||
![]() | Journal: J.Am.Chem.Soc. / Year: 2000 Title: Crystal Structures of Substrate and Inhibitor Complexes with AmpC -Lactamase: Possible Implications for Substrate-Assisted Catalysis Authors: Patera, A. / Blaszczak, L.C. / Shoichet, B.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 144.5 KB | Display | ![]() |
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PDB format | ![]() | 120.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 39587.922 Da / Num. of mol.: 2 / Mutation: Q120L/Y150E Source method: isolated from a genetically manipulated source Details: INHIBITOR MOXALACTAM, RESIDUE MOX, BINDS TO SER 61 BY BREAKING ITS C1-N1 LACTAM BOND Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.53 Å3/Da / Density % sol: 51.34 % | |||||||||||||||
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.7 Details: 1.7 M potassium phosphate, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |||||||||||||||
Crystal grow | *PLUS Temperature: 23 ℃ | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 129 K |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.2→20 Å / Num. all: 39390 / Num. obs: 39390 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.114 / Net I/σ(I): 12.8 |
Reflection shell | Resolution: 2.2→2.25 Å / Rmerge(I) obs: 0.316 |
Reflection | *PLUS % possible obs: 95 % |
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Processing
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Refinement | Resolution: 2.2→8 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.2→8 Å
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Software | *PLUS Name: CNS / Classification: refinement | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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