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Yorodumi- PDB-1f7g: SOLUTION STRUCTURE OF THE RNASE P RNA (M1 RNA) P4 STEM C70U MUTAN... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1f7g | ||||||
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Title | SOLUTION STRUCTURE OF THE RNASE P RNA (M1 RNA) P4 STEM C70U MUTANT OLIGORIBONUCLEOTIDE, ENSEMBLE OF 17 STRUCTURES | ||||||
Components | RNASE P RNA RIBOZYME, P4 DOMAIN | ||||||
Keywords | RNA / RIBONUCLEASE P / RIBOZYME / TRANSFER RNA PROCESSING / P4 STEM / C70U MUTANT / METAL BINDING SITE | ||||||
Function / homology | RNA / RNA (> 10) Function and homology information | ||||||
Method | SOLUTION NMR / restrained molecular dynamics; simulated annealing | ||||||
Authors | Schmitz, M. / Tinoco Jr., I. | ||||||
Citation | Journal: RNA / Year: 2000 Title: Solution structure and metal-ion binding of the P4 element from bacterial RNase P RNA. Authors: Schmitz, M. / Tinoco Jr., I. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f7g.cif.gz | 277.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f7g.ent.gz | 244.1 KB | Display | PDB format |
PDBx/mmJSON format | 1f7g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f7/1f7g ftp://data.pdbj.org/pub/pdb/validation_reports/f7/1f7g | HTTPS FTP |
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-Related structure data
Related structure data | 1f6xC 1f6zC 1f78C 1f79C 1f7fC 1f7hC 1f7iC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 8634.127 Da / Num. of mol.: 1 / Fragment: P4 STEM / Mutation: C70U / Source method: obtained synthetically Details: synthesized from DNA oligonucleotide template by T7 RNA polymerase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques and 13C and 31P heteronuclear techniques at natural abundance |
-Sample preparation
Details |
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: restrained molecular dynamics; simulated annealing / Software ordinal: 1 Details: The structures are based on a total of 268 NOE derived distance constraints, 171 dihedral restraints and 49 distance restraints from hydrogen bonds. The 17 structures with lowest NOE and ...Details: The structures are based on a total of 268 NOE derived distance constraints, 171 dihedral restraints and 49 distance restraints from hydrogen bonds. The 17 structures with lowest NOE and dihedral angle violation energies are presented | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 50 / Conformers submitted total number: 17 |