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- PDB-1f21: DIVALENT METAL COFACTOR BINDING IN THE KINETIC FOLDING TRAJECTORY... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1f21 | ||||||
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Title | DIVALENT METAL COFACTOR BINDING IN THE KINETIC FOLDING TRAJECTORY OF E. COLI RIBONUCLEASE HI | ||||||
![]() | RIBONUCLEASE HI | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Goedken, E.R. / Keck, J.L. / Berger, J.M. / Marqusee, S. | ||||||
![]() | ![]() Title: Divalent metal cofactor binding in the kinetic folding trajectory of Escherichia coli ribonuclease HI. Authors: Goedken, E.R. / Keck, J.L. / Berger, J.M. / Marqusee, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 44.9 KB | Display | ![]() |
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PDB format | ![]() | 31.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17526.805 Da / Num. of mol.: 1 / Mutation: C13A, C63A, C133A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.85 Å3/Da / Density % sol: 33.66 % | ||||||||||||||||||||
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Crystal grow![]() | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: Hepes, pH 7.5, PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K | ||||||||||||||||||||
Crystal grow | *PLUS PH range low: 8 / PH range high: 7 | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Nov 11, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.4→20 Å / Num. all: 26582 / Num. obs: 24663 / % possible obs: 92.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 21.1 % / Biso Wilson estimate: 15.6 Å2 / Rmerge(I) obs: 0.065 / Net I/σ(I): 16.7 |
Reflection shell | Resolution: 1.4→1.45 Å / Redundancy: 4 % / Rmerge(I) obs: 0.136 / Num. unique all: 2603 / % possible all: 59.5 |
Reflection | *PLUS Rmerge(I) obs: 0.063 |
Reflection shell | *PLUS % possible obs: 59.8 % / Mean I/σ(I) obs: 9.8 |
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Processing
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Refinement | Resolution: 1.4→20 Å / σ(F): 0 / σ(I): 0
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Refinement step | Cycle: LAST / Resolution: 1.4→20 Å
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Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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