+Open data
-Basic information
Entry | Database: PDB / ID: 1eyv | ||||||
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Title | THE CRYSTAL STRUCTURE OF NUSB FROM MYCOBACTERIUM TUBERCULOSIS | ||||||
Components | N-UTILIZING SUBSTANCE PROTEIN B HOMOLOG | ||||||
Keywords | TRANSCRIPTION / helical bundle / Structural Genomics / PSI / Protein Structure Initiative / TB Structural Genomics Consortium / TBSGC | ||||||
Function / homology | Function and homology information transcription antitermination / DNA-templated transcription termination / RNA binding / cytosol Similarity search - Function | ||||||
Biological species | Mycobacterium tuberculosis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.6 Å | ||||||
Authors | Gopal, B. / Haire, L.F. / Cox, R.A. / Colston, M.J. / Major, S. / Brannigan, J.A. / Smerdon, S.J. / Dodson, G.G. / TB Structural Genomics Consortium (TBSGC) | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2000 Title: The crystal structure of NusB from Mycobacterium tuberculosis. Authors: Gopal, B. / Haire, L.F. / Cox, R.A. / Colston, M.J. / Major, S. / Brannigan, J.A. / Smerdon, S.J. / Dodson, G. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2000 Title: Crystallization and preliminary X-ray diffraction studies on the N-utilizing substance-B (NusB) from Mycobacterium tuberculosis. Authors: Gopal, B. / Cox, R.A. / Colston, M.J. / Dodson, G.G. / Smerdon, S.J. / Haire, L.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1eyv.cif.gz | 67.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1eyv.ent.gz | 51.1 KB | Display | PDB format |
PDBx/mmJSON format | 1eyv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ey/1eyv ftp://data.pdbj.org/pub/pdb/validation_reports/ey/1eyv | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological assembly is a dimer constructed from chain A a symmetry partner generated by the two-fold. |
-Components
#1: Protein | Mass: 16765.174 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) / Plasmid: PET-15B / Production host: Escherichia coli (E. coli) / References: UniProt: P95020, UniProt: P9WIV1*PLUS #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 2 |
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-Sample preparation
Crystal | Density Matthews: 1.98 Å3/Da / Density % sol: 37.75 % | |||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 2.0 M ammonium sulfate, 0.1 M Na HEPES buffer, pH 7.5, 2 % PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 22K | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 291 KDetails: Gopal, B., (2000) Acta Crystallogr., Sect.D, 56, 64. | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction |
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Diffraction source |
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Detector | Date: May 26, 1999 | |||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 | |||||||||||||||
Reflection | Resolution: 1.6→15 Å / Num. all: 1501196 / Num. obs: 34118 / % possible obs: 95.5 % / Observed criterion σ(F): 2 / Redundancy: 4.4 % / Biso Wilson estimate: 16.7 Å2 / Rmerge(I) obs: 0.052 | |||||||||||||||
Reflection shell | Resolution: 1.6→1.63 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.331 / Num. unique all: 1328 / % possible all: 75 | |||||||||||||||
Reflection shell | *PLUS % possible obs: 75 % |
-Processing
Software |
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Refinement | Resolution: 1.6→15 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.6→15 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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