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Yorodumi- PDB-1eft: THE CRYSTAL STRUCTURE OF ELONGATION FACTOR EF-TU FROM THERMUS AQU... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1eft | ||||||
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Title | THE CRYSTAL STRUCTURE OF ELONGATION FACTOR EF-TU FROM THERMUS AQUATICUS IN THE GTP CONFORMATION | ||||||
Components | ELONGATION FACTOR TUEF-Tu | ||||||
Keywords | ELONGATION FACTOR | ||||||
Function / homology | Function and homology information translation elongation factor activity / GTPase activity / GTP binding / cytoplasm Similarity search - Function | ||||||
Biological species | Thermus aquaticus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.5 Å | ||||||
Authors | Kjeldgaard, M. / Nissen, P. / Thirup, S. / Nyborg, J. | ||||||
Citation | Journal: Structure / Year: 1993 Title: The crystal structure of elongation factor EF-Tu from Thermus aquaticus in the GTP conformation. Authors: Kjeldgaard, M. / Nissen, P. / Thirup, S. / Nyborg, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1eft.cif.gz | 94.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1eft.ent.gz | 70.7 KB | Display | PDB format |
PDBx/mmJSON format | 1eft.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ef/1eft ftp://data.pdbj.org/pub/pdb/validation_reports/ef/1eft | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 44728.953 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus aquaticus (bacteria) / References: UniProt: Q01698 |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-GNP / |
#4: Water | ChemComp-HOH / |
Sequence details | SEQUENCE ADVISORY NOTICE DIFFERENCE BETWEEN SWISS-PROT AND PDB SEQUENCE. SWISS-PROT ENTRY NAME: ...SEQUENCE ADVISORY NOTICE DIFFERENCE |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.36 Å3/Da / Density % sol: 63.39 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 19 ℃ / pH: 7.7 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ROTATING ANODE / Wavelength: 1.5418 |
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Radiation | Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.2 Å |
Reflection shell | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 2.32 Å / % possible obs: 74.2 % / Num. unique obs: 1443 / Rmerge(I) obs: 0.45 |
-Processing
Software |
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Refinement | Resolution: 2.5→8 Å / σ(F): 0 /
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Refinement step | Cycle: LAST / Resolution: 2.5→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 1.3 |