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- PDB-1ebl: THE 1.8 A CRYSTAL STRUCTURE AND ACTIVE SITE ARCHITECTURE OF BETA-... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ebl | ||||||
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Title | THE 1.8 A CRYSTAL STRUCTURE AND ACTIVE SITE ARCHITECTURE OF BETA-KETOACYL-[ACYL CARRIER PROTEIN] SYNTHASE III (FABH) FROM ESCHERICHIA COLI | ||||||
![]() | BETA-KETOACYL-ACP SYNTHASE III![]() | ||||||
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Function / homology | ![]() beta-ketoacyl-[acyl-carrier-protein] synthase III / beta-ketoacyl-acyl-carrier-protein synthase III activity / 3-oxoacyl-[acyl-carrier-protein] synthase activity / fatty acid metabolic process / fatty acid biosynthetic process / ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Davies, C. / Heath, R.J. / White, S.W. / Rock, C.O. | ||||||
![]() | ![]() Title: The 1.8 A crystal structure and active-site architecture of beta-ketoacyl-acyl carrier protein synthase III (FabH) from escherichia coli. Authors: Davies, C. / Heath, R.J. / White, S.W. / Rock, C.O. #1: ![]() Title: Isolation and characterization of the beta-ketoacyl-acyl carrier protein synthase III gene (fabH) from Escherichia coli K-12 Authors: Tsay, J.T. / Oh, W. / Larson, T.J. / Jackowski, S. / Rock, C.O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 144.8 KB | Display | ![]() |
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PDB format | ![]() | 119.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.33103, -0.40231, -0.85256), Vector ![]() Details | The biological assembly is a dimer constructed from chain A and chain B as represented in the crystal asymmetric unit. | |
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Components
#1: Protein | ![]() Mass: 33923.129 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() References: UniProt: P0A6R0, beta-ketoacyl-[acyl-carrier-protein] synthase I #2: Chemical | ![]() #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.42 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 1.8-2.0 M ammonium sulphate, 2% PEG 400, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K | ||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 50.5 % | ||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.4 | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: OTHER / Detector: CCD / Date: Jan 21, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.8→31.6 Å / Num. all: 230489 / Num. obs: 61752 / % possible obs: 97.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.73 % / Biso Wilson estimate: 17.17 Å2 / Rmerge(I) obs: 0.056 / Net I/σ(I): 20.52 |
Reflection shell | Resolution: 1.8→1.89 Å / Redundancy: 2.88 % / Rmerge(I) obs: 0.14 / Num. unique all: 8193 / % possible all: 89.8 |
Reflection | *PLUS Num. measured all: 230489 |
Reflection shell | *PLUS % possible obs: 89.8 % |
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Processing
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Refinement | Resolution: 1.8→20 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber Details: Restrained refinement with maximum likelihood residual, Minimization by sparse matrix
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Refinement step | Cycle: LAST / Resolution: 1.8→20 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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