+Open data
-Basic information
Entry | Database: PDB / ID: 1ea9 | ||||||
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Title | Cyclomaltodextrinase | ||||||
Components | CYCLOMALTODEXTRINASE | ||||||
Keywords | HYDROLASE / GLYCOSIDASE | ||||||
Function / homology | Function and homology information cyclomaltodextrinase / cyclomaltodextrinase activity / carbohydrate metabolic process Similarity search - Function | ||||||
Biological species | BACILLUS SP. (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.2 Å | ||||||
Authors | Cho, H.-S. / Kim, M.-S. / Oh, B.-H. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2002 Title: Cyclomaltodextrinase, Neopullulanase, and Maltogenic Amylase are Nearly Indistinguishable from Each Other Authors: Lee, H.-S. / Kim, M.-S. / Cho, H.-S. / Kim, J.-I. / Kim, T.-J. / Choi, J.-H. / Park, C. / Lee, H.-S. / Oh, B.-H. / Park, K.-H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ea9.cif.gz | 234.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ea9.ent.gz | 191.2 KB | Display | PDB format |
PDBx/mmJSON format | 1ea9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ea/1ea9 ftp://data.pdbj.org/pub/pdb/validation_reports/ea/1ea9 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 67754.359 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) BACILLUS SP. (bacteria) / References: UniProt: Q59226, cyclomaltodextrinase |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 5.76 Å3/Da / Density % sol: 78.64 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.50 | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 22 ℃ / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: PAL/PLS / Beamline: 6B / Wavelength: 1 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→30 Å / Num. obs: 42505 / % possible obs: 90.2 % / Observed criterion σ(I): 0.1 / Redundancy: 2.5 % / Rmerge(I) obs: 0.063 / Net I/σ(I): 12.29 |
Reflection | *PLUS Lowest resolution: 20 Å / Num. obs: 51030 / Num. measured all: 671314 |
Reflection shell | *PLUS % possible obs: 85.8 % / Rmerge(I) obs: 0.178 |
-Processing
Software | Name: CNS / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.2→10 Å / σ(F): 1
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Refinement step | Cycle: LAST / Resolution: 3.2→10 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 10 Å / % reflection Rfree: 5 % / Rfactor Rfree: 0.256 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_bond_d / Dev ideal: 0.0055 |