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- PDB-1e8h: STRUCTURE OF THE H61T MUTANT OF THE FLAVOENZYME VANILLYL-ALCOHOL ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1e8h | ||||||
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Title | STRUCTURE OF THE H61T MUTANT OF THE FLAVOENZYME VANILLYL-ALCOHOL OXIDASE IN THE APO FORM COMPLEXED BY ADP | ||||||
![]() | VANILLYL-ALCOHOL OXIDASE![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Mattevi, A. / Fraaije, M.W. | ||||||
![]() | ![]() Title: Structural Analysis of Flavinylation in Vanillyl-Alcohol Oxidase Authors: Fraaije, M.W. / Van Der Heuvel, R.H.H. / Van Berkel, W.J.H. / Mattevi, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 226 KB | Display | ![]() |
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PDB format | ![]() | 179.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1e8fC ![]() 1e8gC ![]() 1qltS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.238137, -0.971199, 0.007873), Vector ![]() Details | BIOLOGICAL_UNIT: HOMOOCTAMER | |
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Components
#1: Protein | ![]() Mass: 62969.934 Da / Num. of mol.: 2 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() #2: Chemical | ![]() #3: Water | ChemComp-HOH / | ![]() Compound details | CATALYZES THE CONVERSION OF VANILLIN ALCOHOL TO VANILLIN, AND A WIDE RANGE OF PHENOLIC COMPOUNDS OF ...CATALYZES THE CONVERSION | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 45.56 % | |||||||||||||||
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Crystal grow![]() | pH: 4.6 / Details: FROM 6% PEG4000, 100 MM ACETATE BUFFER PH 4.6 | |||||||||||||||
Crystal grow | *PLUS pH: 5.1 / Method: vapor diffusion, hanging drop | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Feb 15, 1999 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.6→20 Å / Num. obs: 34320 / % possible obs: 99.8 % / Observed criterion σ(I): 0 / Redundancy: 2.5 % / Rmerge(I) obs: 0.09 / Rsym value: 0.09 / Net I/σ(I): 6 |
Reflection shell | Resolution: 2.6→2.7 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.393 / Mean I/σ(I) obs: 2.8 / Rsym value: 0.393 / % possible all: 100 |
Reflection | *PLUS Num. measured all: 85638 |
Reflection shell | *PLUS % possible obs: 100 % |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1QLT Resolution: 2.6→20 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.6→20 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: p_plane_restr / Dev ideal: 0.01 |