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Open data
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Basic information
Entry | Database: PDB / ID: 1dwr | ||||||
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Title | MYOGLOBIN (HORSE HEART) WILD-TYPE COMPLEXED WITH CO | ||||||
![]() | Myoglobin![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chu, K. / Vojtechovsky, J. / McMahon, B.H. / Sweet, R.M. / Berendzen, J. / Schlichting, I. | ||||||
![]() | ![]() Title: Crystal Structure of a New Ligand Binding Intermediate in Wildtype Carbonmonoxy Myoglobin Authors: Chu, K. / Vojtechovsky, J. / Mcmahon, B.H. / Sweet, R.M. / Berendzen, J. / Schlichting, I. #1: ![]() Title: A Myoglobin Variant with a Polar Substitution in a Conserved Hydrophobic Cluster in the Heme Binding Pocket Authors: Maurus, R. / Overall, C.M. / Bogumil, R. / Luo, Y. / Mauk, A.G. / Smith, M. / Brayer, G.D. #2: ![]() Title: Crystal Structure of Photolysed Myoglobin Authors: Schlichting, I. / Berendzen, J. / Phillips Jr, G.N. / Sweet, R.M. #3: Journal: J.Mol.Biol. / Year: 1987 Title: Crystallization and Preliminary Diffraction Data for Horse Heart Metmyoglobin Authors: Sherwood, C. / Mauk, A.G. / Brayer, G.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 49.1 KB | Display | ![]() |
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PDB format | ![]() | 33.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1dwsC ![]() 1dwtC ![]() 1aziS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | BIOLOGICAL_UNIT: MONOMER |
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Components
#1: Protein | ![]() Mass: 16983.514 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-HEM / ![]() | ||
#3: Chemical | ChemComp-CMO / ![]() | ||
#4: Chemical | ![]() #5: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.84 Å3/Da / Density % sol: 33.18 % | ||||||||||||||||||||||||||||||
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Crystal grow![]() | Method: vapor diffusion, hanging drop / pH: 7.5 Details: HHMB(SIGMA) WAS CRYSTALLIZED AT ROOM TEMPERATURE BY EQUILIBRATING 10 UL DROPS OF 5 MG/ML PROTEIN IN 1.7-1.8 M AMMONIUM SULFATE, 0.1 M TRIS HCL PH 7.5 USING THE HANGING DROP GEOMETRY | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PRINCETON SCIENTIFIC / Detector: CCD / Date: Jun 2, 1998 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.4→20 Å / Num. obs: 23795 / % possible obs: 96.1 % / Observed criterion σ(I): 0 / Redundancy: 2.47 % / Rmerge(I) obs: 0.07 / Rsym value: 0.07 / Net I/σ(I): 9.2 |
Reflection shell | Resolution: 1.4→1.5 Å / Redundancy: 1.6 % / Rmerge(I) obs: 0.227 / Mean I/σ(I) obs: 2.3 / Rsym value: 0.227 / % possible all: 87.2 |
Reflection shell | *PLUS % possible obs: 87.2 % |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1AZI Resolution: 1.45→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Details: LYS47 AND SER92 HAVE ALTERNATE CONFORMATIONS
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Refinement step | Cycle: LAST / Resolution: 1.45→20 Å
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Refine LS restraints |
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