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Yorodumi- PDB-1cz7: THE CRYSTAL STRUCTURE OF A MINUS-END DIRECTED MICROTUBULE MOTOR P... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1cz7 | ||||||
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Title | THE CRYSTAL STRUCTURE OF A MINUS-END DIRECTED MICROTUBULE MOTOR PROTEIN NCD REVEALS VARIABLE DIMER CONFORMATIONS | ||||||
Components | MICROTUBULE MOTOR PROTEIN NCD | ||||||
Keywords | CONTRACTILE PROTEIN / NCD CRYSTAL STRUCTURE / MICROTUBULE MOTORS / KINESIN SUPERFAMILY | ||||||
Function / homology | Function and homology information minus-end directed microtubule sliding / distributive segregation / regulation of mitotic spindle elongation / meiotic spindle assembly / mitotic spindle elongation / mitotic spindle microtubule / meiotic spindle organization / minus-end-directed microtubule motor activity / spindle assembly involved in female meiosis / regulation of mitotic spindle assembly ...minus-end directed microtubule sliding / distributive segregation / regulation of mitotic spindle elongation / meiotic spindle assembly / mitotic spindle elongation / mitotic spindle microtubule / meiotic spindle organization / minus-end-directed microtubule motor activity / spindle assembly involved in female meiosis / regulation of mitotic spindle assembly / microtubule bundle formation / mitotic centrosome separation / meiotic spindle / spindle organization / mitotic spindle assembly / mRNA transport / mitotic spindle organization / chromosome segregation / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / spindle / microtubule binding / hydrolase activity / cell division / centrosome / protein homodimerization activity / ATP binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.9 Å | ||||||
Authors | Kozielski, F.K. / De Bonis, S. / Burmeister, W. / Cohen-Addad, C. / Wade, R. | ||||||
Citation | Journal: Structure Fold.Des. / Year: 1999 Title: The crystal structure of the minus-end-directed microtubule motor protein ncd reveals variable dimer conformations. Authors: Kozielski, F. / De Bonis, S. / Burmeister, W.P. / Cohen-Addad, C. / Wade, R.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1cz7.cif.gz | 295.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1cz7.ent.gz | 238.2 KB | Display | PDB format |
PDBx/mmJSON format | 1cz7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1cz7_validation.pdf.gz | 628.7 KB | Display | wwPDB validaton report |
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Full document | 1cz7_full_validation.pdf.gz | 724.3 KB | Display | |
Data in XML | 1cz7_validation.xml.gz | 44 KB | Display | |
Data in CIF | 1cz7_validation.cif.gz | 61.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cz/1cz7 ftp://data.pdbj.org/pub/pdb/validation_reports/cz/1cz7 | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 46117.004 Da / Num. of mol.: 4 / Fragment: CONSTRUCT MC5 FROM NCD Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Plasmid: PET / Production host: Escherichia coli (E. coli) / References: UniProt: P20480 #2: Chemical | ChemComp-MG / #3: Chemical | ChemComp-ADP / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.06 Å3/Da / Density % sol: 59.85 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 6.2 Details: PEG 4000, NACL, MGCL2, K3PO4, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 58.6 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 6.8 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 0.928 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Dec 20, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.928 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→40 Å / Num. all: 50539 / Num. obs: 48773 / % possible obs: 96.5 % / Observed criterion σ(I): 1 / Rmerge(I) obs: 0.085 |
Reflection shell | Highest resolution: 2.9 Å |
Reflection | *PLUS Num. measured all: 136320 |
Reflection shell | *PLUS Lowest resolution: 3 Å / Rmerge(I) obs: 0.28 |
-Processing
Software |
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Refinement | Resolution: 2.9→40 Å / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: ENGH & HUBER / Details: SIMULATED ANNEALING, NCS
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.9→40 Å
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Software | *PLUS Name: CNS / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.9 Å / Lowest resolution: 40 Å / σ(F): 2 / % reflection Rfree: 5 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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