+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1cm0 | ||||||
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タイトル | CRYSTAL STRUCTURE OF THE PCAF/COENZYME-A COMPLEX | ||||||
要素 | P300/CBP ASSOCIATING FACTOR | ||||||
キーワード | SIGNALING PROTEIN / P300/CBP ASSOCIATED FACTOR / COENZYME A (補酵素A) / ACETYLTRANSFERASE / COACTIVATOR (コアクチベーター) | ||||||
機能・相同性 | 機能・相同性情報 regulation of protein ADP-ribosylation / negative regulation of rRNA processing / histone H3K9 acetyltransferase activity / diamine N-acetyltransferase / diamine N-acetyltransferase activity / positive regulation of transcription from RNA polymerase II promoter by glucose / negative regulation of centriole replication / Physiological factors / positive regulation of attachment of mitotic spindle microtubules to kinetochore / peptidyl-lysine acetylation ...regulation of protein ADP-ribosylation / negative regulation of rRNA processing / histone H3K9 acetyltransferase activity / diamine N-acetyltransferase / diamine N-acetyltransferase activity / positive regulation of transcription from RNA polymerase II promoter by glucose / negative regulation of centriole replication / Physiological factors / positive regulation of attachment of mitotic spindle microtubules to kinetochore / peptidyl-lysine acetylation / lysine N-acetyltransferase activity, acting on acetyl phosphate as donor / YAP1- and WWTR1 (TAZ)-stimulated gene expression / positive regulation of fatty acid biosynthetic process / ミオフィラメント / histone H3 acetyltransferase activity / internal peptidyl-lysine acetylation / cyclin-dependent protein serine/threonine kinase inhibitor activity / N-terminal peptidyl-lysine acetylation / ATAC complex / I band / cellular response to parathyroid hormone stimulus / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / SAGA complex / RUNX3 regulates NOTCH signaling / limb development / A band / NOTCH4 Intracellular Domain Regulates Transcription / Regulation of FOXO transcriptional activity by acetylation / NOTCH3 Intracellular Domain Regulates Transcription / regulation of tubulin deacetylation / peptide-lysine-N-acetyltransferase activity / histone acetyltransferase binding / protein acetylation / Notch-HLH transcription pathway / Formation of WDR5-containing histone-modifying complexes / regulation of cell division / Formation of paraxial mesoderm / acetyltransferase activity / RNA Polymerase I Transcription Initiation / regulation of RNA splicing / regulation of embryonic development / regulation of DNA repair / histone acetyltransferase activity / positive regulation of gluconeogenesis / ヒストンアセチルトランスフェラーゼ / transcription initiation-coupled chromatin remodeling / positive regulation of glycolytic process / 糖新生 / transcription coregulator activity / RNA polymerase II transcription regulatory region sequence-specific DNA binding / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / B-WICH complex positively regulates rRNA expression / 紡錘体 / NOTCH1 Intracellular Domain Regulates Transcription / Metalloprotease DUBs / 動原体 / 記憶 / Pre-NOTCH Transcription and Translation / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / positive regulation of neuron projection development / histone deacetylase binding / cellular response to insulin stimulus / vasodilation / rhythmic process / cellular response to oxidative stress / heart development / HATs acetylate histones / DNA-binding transcription factor binding / Estrogen-dependent gene expression / transcription coactivator activity / regulation of cell cycle / クロマチンリモデリング / 細胞周期 / negative regulation of cell population proliferation / 中心体 / chromatin binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / protein kinase binding / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / 核質 / 細胞核 / 細胞質基質 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 2.3 Å | ||||||
データ登録者 | Clements, A. / Rojas, J.R. / Trievel, R.C. / Wang, L. / Berger, S.L. / Marmorstein, R. | ||||||
引用 | ジャーナル: EMBO J. / 年: 1999 タイトル: Crystal structure of the histone acetyltransferase domain of the human PCAF transcriptional regulator bound to coenzyme A. 著者: Clements, A. / Rojas, J.R. / Trievel, R.C. / Wang, L. / Berger, S.L. / Marmorstein, R. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1cm0.cif.gz | 81.8 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1cm0.ent.gz | 62.4 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1cm0.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/cm/1cm0 ftp://data.pdbj.org/pub/pdb/validation_reports/cm/1cm0 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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単位格子 |
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-要素
#1: タンパク質 | 分子量: 19508.889 Da / 分子数: 2 / 断片: HISTONE ACETYLTRANSFERASE DOMAIN / 由来タイプ: 組換発現 / 詳細: COCRYSTALLIZED WITH COENZYME A / 由来: (組換発現) Homo sapiens (ヒト) / 細胞内の位置: NUCLEUS細胞核 / プラスミド: PRSET A / 生物種 (発現宿主): Escherichia coli / 細胞内の位置 (発現宿主): CYTOPLASM / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): BL21(DE3) / 参照: UniProt: Q92831 #2: 化合物 | #3: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.8 Å3/Da / 溶媒含有率: 42 % | |||||||||||||||||||||||||||||||||||
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結晶化 | 温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8.5 詳細: 5MG/ML OF P/CAF WITH 2 M EXCESS COFACTOR, 100MM TRIS, 1.5 M LITHIUM SULFATE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 温度: 20 ℃詳細: drop consists of equal amounts of protein and reservoir solutions | |||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: シンクロトロン / サイト: NSLS / ビームライン: X4A / 波長: 1.009 |
検出器 | タイプ: RIGAKU RAXIS IV / 検出器: IMAGE PLATE |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.009 Å / 相対比: 1 |
反射 | 解像度: 2.3→20 Å / Num. obs: 17943 / % possible obs: 96.5 % / Observed criterion σ(I): 0 / 冗長度: 5.3 % / Biso Wilson estimate: 41.6 Å2 / Rsym value: 4 / Net I/σ(I): 18 |
反射 シェル | 解像度: 2.3→2.7 Å / 冗長度: 2.3 % / Mean I/σ(I) obs: 4.8 / Rsym value: 15.5 / % possible all: 99.8 |
反射 | *PLUS Num. measured all: 94731 / Rmerge(I) obs: 0.04 |
反射 シェル | *PLUS % possible obs: 99.8 % / Rmerge(I) obs: 0.155 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: APOTGCN5 解像度: 2.3→20 Å / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 0
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溶媒の処理 | Bsol: 39 Å2 / ksol: 0.35 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 36.5 Å2
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精密化ステップ | サイクル: LAST / 解像度: 2.3→20 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.3→2.32 Å / Total num. of bins used: 35
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Xplor file |
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ソフトウェア | *PLUS 名称: CNS / 分類: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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