[English] 日本語
Yorodumi- PDB-1cks: HUMAN CKSHS2 ATOMIC STRUCTURE: A ROLE FOR ITS HEXAMERIC ASSEMBLY ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1cks | ||||||
---|---|---|---|---|---|---|---|
Title | HUMAN CKSHS2 ATOMIC STRUCTURE: A ROLE FOR ITS HEXAMERIC ASSEMBLY IN CELL CYCLE CONTROL | ||||||
Components | CYCLIN-DEPENDENT KINASE SUBUNIT, TYPE 2 | ||||||
Keywords | CELL DIVISION | ||||||
Function / homology | Function and homology information meiosis I / mitotic cell cycle phase transition / cyclin-dependent protein serine/threonine kinase activator activity / SCF ubiquitin ligase complex / cyclin-dependent protein kinase holoenzyme complex / regulation of mitotic cell cycle / ubiquitin binding / histone binding / fibroblast proliferation / cell division ...meiosis I / mitotic cell cycle phase transition / cyclin-dependent protein serine/threonine kinase activator activity / SCF ubiquitin ligase complex / cyclin-dependent protein kinase holoenzyme complex / regulation of mitotic cell cycle / ubiquitin binding / histone binding / fibroblast proliferation / cell division / chromatin binding / regulation of transcription by RNA polymerase II / protein kinase binding Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.1 Å | ||||||
Authors | Parge, H.E. / Arvai, A.S. / Tainer, J.A. | ||||||
Citation | Journal: Science / Year: 1993 Title: Human CksHs2 atomic structure: a role for its hexameric assembly in cell cycle control. Authors: Parge, H.E. / Arvai, A.S. / Murtari, D.J. / Reed, S.I. / Tainer, J.A. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1cks.cif.gz | 65.7 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1cks.ent.gz | 50.1 KB | Display | PDB format |
PDBx/mmJSON format | 1cks.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ck/1cks ftp://data.pdbj.org/pub/pdb/validation_reports/ck/1cks | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||
Unit cell |
| ||||||||||||
Noncrystallographic symmetry (NCS) | NCS oper:
| ||||||||||||
Details | THE FOLLOWING TRANSFORMATION WILL GENERATE CHAINS D, E, F FROM CHAINS A, B, C, RESPECTIVELY. CHAINS A, B, C, D, E, AND F FORM A HEXAMER. TRNSF1 3 -1.000000 0.000000 0.000000 53.094002 TRNSF2 3 0.000000 1.000000 0.000000 0.000000 TRNSF3 3 0.000000 0.000000 -1.000000 90.760002 |
-Components
#1: Protein | Mass: 9879.365 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / References: UniProt: P33552 #2: Chemical | #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.22 % | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | |||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Radiation | Scattering type: x-ray |
---|---|
Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.1 Å / Num. obs: 15146 / % possible obs: 99.9 % / Observed criterion σ(I): 3.2 / Num. measured all: 93995 / Rmerge(I) obs: 0.069 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2.1→10 Å / σ(F): 3 /
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→10 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.168 / Rfactor Rwork: 0.168 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 3.4 |