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Yorodumi- PDB-1cfr: CRYSTAL STRUCTURE OF CITROBACTER FREUNDII RESTRICTION ENDONUCLEAS... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1cfr | ||||||
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Title | CRYSTAL STRUCTURE OF CITROBACTER FREUNDII RESTRICTION ENDONUCLEASE CFR10I AT 2.15 ANGSTROMS RESOLUTION. | ||||||
Components | RESTRICTION ENDONUCLEASERestriction enzyme | ||||||
Keywords | RESTRICTION ENDONUCLEASE / RESTRICTION ENZYME | ||||||
Function / homology | Function and homology information type II site-specific deoxyribonuclease / type II site-specific deoxyribonuclease activity / DNA restriction-modification system / metal ion binding Similarity search - Function | ||||||
Biological species | Citrobacter freundii (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 2.15 Å | ||||||
Authors | Bozic, D. / Grazulis, S. / Siksnys, V. / Huber, R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1996 Title: Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15 A resolution. Authors: Bozic, D. / Grazulis, S. / Siksnys, V. / Huber, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1cfr.cif.gz | 82 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1cfr.ent.gz | 62.7 KB | Display | PDB format |
PDBx/mmJSON format | 1cfr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cf/1cfr ftp://data.pdbj.org/pub/pdb/validation_reports/cf/1cfr | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 32051.387 Da / Num. of mol.: 1 / Fragment: RESIDUES 1 - 283 Source method: isolated from a genetically manipulated source Details: CHARACTERIZATION OF CFR10I RESTRICTION ENDONUCLEASE, JANULAITIS ET AL. (1983) Source: (gene. exp.) Citrobacter freundii (bacteria) / Strain: RFL10I / Plasmid: PBR327 / Production host: Escherichia coli (E. coli) / Strain (production host): RR1 References: UniProt: P56200, type II site-specific deoxyribonuclease |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 49.75 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 / Details: pH 6.5 | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: SYNCHROTRON / Site: MPG/DESY, HAMBURG / Beamline: BW6 / Wavelength: 0.96 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Monochromator: MIRROR / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.96 Å / Relative weight: 1 |
Reflection | Num. obs: 15745 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 2.15→10 Å / σ(F): 0
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Displacement parameters | Biso mean: 29.34 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.15→10 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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