+Open data
-Basic information
Entry | Database: PDB / ID: 1by0 | ||||||
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Title | N-TERMINAL LEUCINE-REPEAT REGION OF HEPATITIS DELTA ANTIGEN | ||||||
Components | PROTEIN (HEPATITIS DELTA ANTIGEN) | ||||||
Keywords | RNA BINDING PROTEIN / HEPATITIS DELTA ANTIGEN / HELIX / SOLUTION STRUCTURE / RNA BINDING | ||||||
Function / homology | Function and homology information host cell / virion component / viral penetration into host nucleus / symbiont entry into host cell / host cell nucleus / RNA binding Similarity search - Function | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Cheng, J.W. / Lin, I.J. / Lou, Y.C. | ||||||
Citation | Journal: Proteins / Year: 1999 Title: Solution structure and RNA-binding activity of the N-terminal leucine-repeat region of hepatitis delta antigen Authors: Lin, I.J. / Lou, Y.C. / Pai, M.T. / Wu, H.N. / Cheng, J.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1by0.cif.gz | 227.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1by0.ent.gz | 193.3 KB | Display | PDB format |
PDBx/mmJSON format | 1by0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/by/1by0 ftp://data.pdbj.org/pub/pdb/validation_reports/by/1by0 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3475.154 Da / Num. of mol.: 1 / Fragment: RESIDUES 24-50 / Source method: obtained synthetically Details: THE PROTEIN WAS CHEMICALLY SYNTHESIZED FROM THE HEPATITIS D VIRUS References: UniProt: P25989 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: DISTANCE RESTRAINTS WERE REQUIRED FROM NOES ASSIGNED IN A 2D NOESY SPECTRUM WITH 75 MS MIXING TIME. |
-Sample preparation
Sample conditions | pH: 6.8 / Pressure: 1 atm / Temperature: 298 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DMX600 / Manufacturer: Bruker / Model: DMX600 / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST TOTAL ENERGY / Conformers calculated total number: 200 / Conformers submitted total number: 20 |