+Open data
-Basic information
Entry | Database: PDB / ID: 1bwg | ||||||
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Title | DNA TRIPLEX WITH 5' AND 3' JUNCTIONS, NMR, 10 STRUCTURES | ||||||
Components |
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Keywords | DNA / DEOXYRIBONUCLEIC ACID / DNA TRIPLEX / TRIPLEX-DUPLEX JUNCTIONS | ||||||
Function / homology | DNA / DNA (> 10) Function and homology information | ||||||
Method | SOLUTION NMR / RESTRAINED MOLECULAR DYNAMICS | ||||||
Authors | Asensio, J.L. / Brown, T. / Lane, A.N. | ||||||
Citation | Journal: Structure Fold.Des. / Year: 1999 Title: Solution conformation of a parallel DNA triple helix with 5' and 3' triplex-duplex junctions. Authors: Asensio, J.L. / Brown, T. / Lane, A.N. #1: Journal: Biochemistry / Year: 1997 Title: Solution Conformation of an Intramolecular DNA Triplex Containing a Nonnucleotide Linker: Comparison with the DNA Duplex Authors: Bartley, J.P. / Brown, T. / Lane, A.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bwg.cif.gz | 215.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bwg.ent.gz | 178 KB | Display | PDB format |
PDBx/mmJSON format | 1bwg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bw/1bwg ftp://data.pdbj.org/pub/pdb/validation_reports/bw/1bwg | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: DNA chain | Mass: 4353.854 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: DNA chain | Mass: 4206.740 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#3: DNA chain | Mass: 1735.166 Da / Num. of mol.: 1 / Source method: obtained synthetically |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURES WERE DETERMINED FROM NOE AND J-COUPLING DATA ON TWO INTRAMOLECULAR DNA TRIPLE HELICES CONTAINING IDENTICAL CORE SEGMENTS. THE RESTRAINTS WERE GENERATED FOR THE SUPERMOLECULE ...Text: THE STRUCTURES WERE DETERMINED FROM NOE AND J-COUPLING DATA ON TWO INTRAMOLECULAR DNA TRIPLE HELICES CONTAINING IDENTICAL CORE SEGMENTS. THE RESTRAINTS WERE GENERATED FOR THE SUPERMOLECULE CONTAINING THE CORE TRIPLEX REGION AND THE 5' AND 3' DUPLEX EXTEN SIONS. |
-Sample preparation
Sample conditions | Ionic strength: 0.1 / pH: 5 / Pressure: 1 atm / Temperature: 313 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: RESTRAINED MOLECULAR DYNAMICS / Software ordinal: 1 / Details: RESTRAINED MD | |||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATIONS / Conformers calculated total number: 30 / Conformers submitted total number: 10 |