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- PDB-1brv: SOLUTION NMR STRUCTURE OF THE IMMUNODOMINANT REGION OF PROTEIN G ... -

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Basic information

Entry
Database: PDB / ID: 1brv
TitleSOLUTION NMR STRUCTURE OF THE IMMUNODOMINANT REGION OF PROTEIN G OF BOVINE RESPIRATORY SYNCYTIAL VIRUS, 48 STRUCTURES
ComponentsPROTEIN G
KeywordsGLYCOPROTEIN / ATTACHMENT PROTEIN G OF BOVINE RESPIRATORY SYNCYTIAL VIRUS / IMMUNOGLOBULIN-BINDING PROTEIN / TRANSMEMBRANE
Function / homology
Function and homology information


virus-mediated perturbation of host defense response => GO:0019049 / host cell surface / membrane => GO:0016020 / symbiont entry into host cell / virion attachment to host cell / virion membrane / extracellular region
Similarity search - Function
Major surface glycoprotein G / Pneumovirus attachment glycoprotein G
Similarity search - Domain/homology
Major surface glycoprotein G
Similarity search - Component
Biological speciesBovine respiratory syncytial virus
MethodSOLUTION NMR / SEE REMARK 3
AuthorsDoreleijers, J.F. / Langedijk, J.P.M. / Hard, K. / Rullmann, J.A.C. / Boelens, R. / Schaaper, W.M. / Van Oirschot, J.T. / Kaptein, R.
Citation
Journal: Biochemistry / Year: 1996
Title: Solution structure of the immunodominant region of protein G of bovine respiratory syncytial virus.
Authors: Doreleijers, J.F. / Langedijk, J.P. / Hard, K. / Boelens, R. / Rullmann, J.A. / Schaaper, W.M. / van Oirschot, J.T. / Kaptein, R.
#1: Journal: J.Gen.Virol. / Year: 1996
Title: Proposed Three-Dimensional Model for the Attachment Protein G of Respiratory Syncytial Virus
Authors: Langedijk, J.P. / Schaaper, W.M. / Meloen, R.H. / Van Oirschot, J.T.
History
DepositionMar 29, 1996Processing site: BNL
Revision 1.0Jun 5, 1997Provider: repository / Type: Initial release
Revision 1.1Mar 27, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Nov 29, 2017Group: Derived calculations / Other
Category: pdbx_database_status / pdbx_struct_assembly ...pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_conf / struct_conf_type
Item: _pdbx_database_status.process_site

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: PROTEIN G


Theoretical massNumber of molelcules
Total (without water)3,6051
Polymers3,6051
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)48 / 50ERROR FUNCTION DGII
Representative

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Components

#1: Protein/peptide PROTEIN G / / BRSV-G REGION


Mass: 3605.001 Da / Num. of mol.: 1 / Fragment: IMMUNODOMINANT REGION, RESIDUES 158 - 189
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Bovine respiratory syncytial virus (strain 391-2)
Genus: Pneumovirus / Species: Bovine respiratory syncytial virus / Strain: 391-2 / References: UniProt: P22261

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111TOCSY
121NOESY
131HSQC
141DQF-COSY

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Sample preparation

Sample conditionspH: 4.6 / Temperature: 285 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometer
ManufacturerField strength (MHz)Spectrometer-ID
Varian7501
Bruker5002

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Processing

NMR software
NameDeveloperClassification
DISCOVERBIOSYMrefinement
REGINEstructure solution
DGIIstructure solution
DISCOVERstructure solution
RefinementMethod: SEE REMARK 3 / Software ordinal: 1
NMR ensembleConformer selection criteria: ERROR FUNCTION DGII / Conformers calculated total number: 50 / Conformers submitted total number: 48

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