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Open data
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Basic information
Entry | Database: PDB / ID: 1beb | ||||||
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Title | BOVINE BETA-LACTOGLOBULIN, LATTICE X | ||||||
![]() | BETA-LACTOGLOBULIN![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Brownlow, S. / Morais-Cabral, J.H. / Sawyer, L. | ||||||
![]() | ![]() Title: Bovine beta-lactoglobulin at 1.8 A resolution--still an enigmatic lipocalin. Authors: Brownlow, S. / Morais Cabral, J.H. / Cooper, R. / Flower, D.R. / Yewdall, S.J. / Polikarpov, I. / North, A.C. / Sawyer, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 94.3 KB | Display | ![]() |
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PDB format | ![]() | 72.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.14058, -0.80081, -0.58218), Vector ![]() |
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Components
#1: Protein | ![]() Mass: 18329.229 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Details: PROTEIN PURCHASED FROM SIGMA CHEMICALS / Source: (natural) ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / | ![]() #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 42 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Method: batch method / pH: 6.5 Details: 25MG/ML PROTEIN WAS DISSOLVED IN 0.1M SODIUM/ POTASSIUM PHOSPHATE BUFFER (PH 7.0) AND CRYSTALLIZED BY THE BATCH METHOD FROM 2M AMMONIUM SULFATE (PH 6.5) AND 0.4M SODIUM/POTASSIUM PHOSPHATE ...Details: 25MG/ML PROTEIN WAS DISSOLVED IN 0.1M SODIUM/ POTASSIUM PHOSPHATE BUFFER (PH 7.0) AND CRYSTALLIZED BY THE BATCH METHOD FROM 2M AMMONIUM SULFATE (PH 6.5) AND 0.4M SODIUM/POTASSIUM PHOSPHATE BUFFER (PH 7.6)., batch method PH range: 6.5-7.6 | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: batch method | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: XENTRONICS / Detector: AREA DETECTOR / Date: Sep 1, 1988 / Details: COLLIMATOR |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.8→15 Å / Num. obs: 25155 / % possible obs: 97 % / Observed criterion σ(I): 0 / Redundancy: 2.1 % / Biso Wilson estimate: 23.5 Å2 / Rmerge(I) obs: 0.07 / Rsym value: 0.1 / Net I/σ(I): 5 |
Reflection shell | Resolution: 1.8→1.96 Å / Redundancy: 1.2 % / Rmerge(I) obs: 0.07 / Mean I/σ(I) obs: 3 / Rsym value: 0.1 / % possible all: 78 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: B-LG LATTICE Z - SOLVED BY MIR Resolution: 1.8→15 Å / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 25.07 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→15 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: UNRESTRAINED AT END | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 1.8→1.88 Å / Total num. of bins used: 25
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rwork: 0.3 |