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Yorodumi- PDB-1b75: SOLUTION STRUCTURE OF RIBOSOMAL PROTEIN L25 FROM ESCHERICHIA COLI -
+Open data
-Basic information
Entry | Database: PDB / ID: 1b75 | ||||||
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Title | SOLUTION STRUCTURE OF RIBOSOMAL PROTEIN L25 FROM ESCHERICHIA COLI | ||||||
Components | PROTEIN (50S RIBOSOMAL PROTEIN L25) | ||||||
Keywords | RNA BINDING PROTEIN / RIBOSOMAL PROTEIN / RNA-BINDING PROTEIN | ||||||
Function / homology | Function and homology information response to radiation / ribosomal large subunit assembly / cytoplasmic translation / 5S rRNA binding / cytosolic large ribosomal subunit / negative regulation of translation / structural constituent of ribosome / translation / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, SIMULAT | ||||||
Authors | Stoldt, M. / Woehnert, J. / Goerlach, M. / Brown, L.R. | ||||||
Citation | Journal: EMBO J. / Year: 1998 Title: The NMR structure of Escherichia coli ribosomal protein L25 shows homology to general stress proteins and glutaminyl-tRNA synthetases. Authors: Stoldt, M. / Wohnert, J. / Gorlach, M. / Brown, L.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1b75.cif.gz | 321.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1b75.ent.gz | 261.9 KB | Display | PDB format |
PDBx/mmJSON format | 1b75.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b7/1b75 ftp://data.pdbj.org/pub/pdb/validation_reports/b7/1b75 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10713.465 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / Strain: MRE 600 / References: UniProt: P68919 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 3D/4D HETERONUCLEAR EXPERIMENTS |
NMR details | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N LABELED L25. |
-Sample preparation
Sample conditions | Ionic strength: 100 mM KCL / pH: 7.0 / Pressure: 1 atm / Temperature: 298 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY INOVA / Manufacturer: Varian / Model: UNITY INOVA / Field strength: 750 MHz |
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-Processing
NMR software |
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Refinement | Method: DISTANCE GEOMETRY, SIMULAT / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | |||||||||
NMR ensemble | Conformer selection criteria: LOWEST TARGET FUNCTION / Conformers calculated total number: 100 / Conformers submitted total number: 10 |