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- PDB-1b4p: CRYSTAL STRUCTURES OF CLASS MU CHIMERIC GST ISOENZYMES M1-2 AND M2-1 -
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Open data
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Basic information
Entry | Database: PDB / ID: 1b4p | ||||||
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Title | CRYSTAL STRUCTURES OF CLASS MU CHIMERIC GST ISOENZYMES M1-2 AND M2-1 | ||||||
![]() | PROTEIN (GLUTATHIONE S-TRANSFERASE) | ||||||
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Function / homology | ![]() response to catechin / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Xiao, G. / Chen, J. / Armstrong, R.N. / Gilliland, G.L. | ||||||
![]() | ![]() Title: Crystal Structures of Class MU Chimeric GST Isoenzymes M1-2 and M2-1 Authors: Xiao, G. / Liu, X. / Ji, X. / Zhang, P. / Johnson, W.W. / Chen, J. / Parsons, J.F. / Stevens, W.J. / Gilliland, G.L. / Armstrong, R.N. #1: ![]() Title: First-Sphere and Second-Sphere Electrostatic Effects in the Active Site of a Class Mu Glutathione S-Transferease Authors: Ji, X. / Zhang, P. / Johnson, W.W. / Sesay, M.A. / Dickert, L. / Prasad, S.S. / Ammon, H.L. / Armstrong, R.N. / Gilliland, G.L. #2: ![]() Title: Structure and Function of the Xenobiotic Substrate Binding Site of a Glutathione S-Transferease as Revealed by X-Ray Crystallogaphic Anaysis of Product Complexes with the Diastereomers of 9-(S- ...Title: Structure and Function of the Xenobiotic Substrate Binding Site of a Glutathione S-Transferease as Revealed by X-Ray Crystallogaphic Anaysis of Product Complexes with the Diastereomers of 9-(S-Glutathionyl)-10-Hydroxy-9,10- Dihydrophenanthren E Authors: Ji, X. / Zhang, P. / Armstrong, R.N. / Gilliland, G.L. #3: ![]() Title: The Three-Dimensional Structure of a Glutathione S-Transferease from the Mu Gene Class. Structural Analysis of the Binary Complex of Isoenzyme 3-3 and Glutathione at 2.2-A Resolution | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68 KB | Display | ![]() |
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PDB format | ![]() | 49.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1gst S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 25740.738 Da / Num. of mol.: 1 / Fragment: COMPLETE PROTEIN Source method: isolated from a genetically manipulated source Details: SUBSTRATE IS (9S, 10S)-9-(S-GLUTATHIONYL)-10-HYDROXY-9.10-DIHYDROPHENANTHRENE Source: (gene. exp.) ![]() ![]() ![]() Description: THE ENZYME IS A CHIMERIC GST, CONSISTING OF DOMAIN I FROM TYPE M1-1 AND DOMAIN II FROM TYPE M2-2 Gene: CDNA INSERT OF CLONE PGT33MX / Organ: LIVER ![]() ![]() ![]() ![]() ![]() | ||||||
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#2: Chemical | ![]() #3: Chemical | #4: Water | ChemComp-HOH / | ![]() Sequence details | FOR THIS CHIMERIC GST M1-2, THE SEQUENCE IN DOMAIN I IS IDENTICAL TO THAT IN M1-1 AND THE SEQUENCE ...FOR THIS CHIMERIC GST M1-2, THE SEQUENCE IN DOMAIN I IS IDENTICAL TO THAT IN M1-1 AND THE SEQUENCE IN DOMAIN II TO THAT IN M2-2 | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47 % |
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Crystal grow![]() | pH: 8 Details: PROTEIN CONC. 10 MG/ML, 25 MM TRIS BUFFER (PH 8.0), 1 MM EDTA, 0.3% OCTYL BETA- D-GLUCOPYRANOSIDE, 2 MM PRODUCT INHIBITOR (GPS) |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: BRUKER / Detector: AREA DETECTOR / Date: Apr 15, 1996 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.7→20 Å / Num. obs: 27013 / % possible obs: 98.3 % / Observed criterion σ(I): 0 / Redundancy: 8.6 % / Rsym value: 0.088 / Net I/σ(I): 8.6 |
Reflection shell | Resolution: 1.7→1.81 Å / Redundancy: 36 % / Mean I/σ(I) obs: 1.9 / Rsym value: 0.44 / % possible all: 89.7 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1GST ![]() 1gst Resolution: 1.7→6 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.7→6 Å
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Refine LS restraints |
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