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- PDB-1b3i: NMR SOLUTION STRUCTURE OF PLASTOCYANIN FROM THE PHOTOSYNTHETIC PR... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1b3i | ||||||
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Title | NMR SOLUTION STRUCTURE OF PLASTOCYANIN FROM THE PHOTOSYNTHETIC PROKARYOTE, PROCHLOROTHRIX HOLLANDICA (MINIMIZED AVERAGE STRUCTURE) | ||||||
![]() | PROTEIN (PLASTOCYANIN) | ||||||
![]() | ![]() ![]() | ||||||
Function / homology | ![]() electron transporter, transferring electrons from cytochrome b6/f complex of photosystem II activity / plasma membrane-derived thylakoid membrane / copper ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Babu, C.R. / Volkman, B.F. / Bullerjahn, G.S. | ||||||
![]() | ![]() Title: NMR solution structure of plastocyanin from the photosynthetic prokaryote, Prochlorothrix hollandica. Authors: Babu, C.R. / Volkman, B.F. / Bullerjahn, G.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 40.2 KB | Display | ![]() |
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PDB format | ![]() | 27.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 10148.560 Da / Num. of mol.: 1 / Mutation: T2S Source method: isolated from a genetically manipulated source Details: T2S MUTATION INTRODUCED TO CLONE IN EXPRESSION VECTOR Source: (gene. exp.) ![]() ![]() Cellular location (production host): CYTOPLASM - INCLUSION BODIES Gene (production host): PETE / Production host: ![]() ![]() ![]() |
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#2: Chemical | ChemComp-CU1 / ![]() |
Sequence details | T2S - INTRODUCED |
-Experimental details
-Experiment
Experiment | Method: ![]() | ||||||||||||||||
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NMR experiment |
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NMR details | Text: MINIMIZED AVERAGE STRUCTURE. THE STRUCTURE WAS DETERMINED USING TWO- DIMENCIONAL NMR SPECTROSCOPY. WATER SUPPRESSION WAS ACHIEVED WITH A WATERGATE SEQ. WITH A 3-9-19 SELECTIVE INVERSION. |
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Sample preparation
Details | Contents: 90% WATER/10% D2O |
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Sample conditions | Ionic strength: 20mM POTASSIUM PHOSPHATE / pH: 7.0 / Pressure: 1 atm / Temperature: 298 K |
Crystal grow![]() | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DMX750 / Manufacturer: Bruker / Model![]() |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 Details: REFINEMENT DETAILS ARE IN THE SUBMITTED FILE AND IN THE JRNL CITATION ABOVE. THE RESTRAINTS ARE IN THE FILES WITH THE 19 CONFORMERS. | ||||||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST TARGET FUNCTION / Conformers calculated total number: 40 / Conformers submitted total number: 1 |