[English] 日本語
![](img/lk-miru.gif)
- PDB-1ao7: COMPLEX BETWEEN HUMAN T-CELL RECEPTOR, VIRAL PEPTIDE (TAX), AND H... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1ao7 | ||||||
---|---|---|---|---|---|---|---|
Title | COMPLEX BETWEEN HUMAN T-CELL RECEPTOR, VIRAL PEPTIDE (TAX), AND HLA-A 0201 | ||||||
![]() |
| ||||||
![]() | COMPLEX (MHC/VIRAL PEPTIDE/RECEPTOR) / ![]() ![]() | ||||||
Function / homology | ![]() symbiont-mediated perturbation of host exit from mitosis / : / symbiont-mediated perturbation of host cell cycle G0/G1 transition checkpoint / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Garboczi, D.N. / Ghosh, P. / Utz, U. / Fan, Q.R. / Biddison, W.E. / Wiley, D.C. | ||||||
![]() | ![]() Title: Structure of the complex between human T-cell receptor, viral peptide and HLA-A2. Authors: Garboczi, D.N. / Ghosh, P. / Utz, U. / Fan, Q.R. / Biddison, W.E. / Wiley, D.C. #1: ![]() Title: Assembly, Specific Binding, and Crystallization of a Human Tcr-Alphabeta with an Antigenic Tax Peptide from Human T Lymphotropic Virus Type 1 and the Class I Mhc Molecule Hla-A2 Authors: Garboczi, D.N. / Utz, U. / Ghosh, P. / Seth, A. / Kim, J. / Vantienhoven, E.A. / Biddison, W.E. / Wiley, D.C. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 156.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 122.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 31854.203 Da / Num. of mol.: 1 / Fragment: EXTRACELLULAR DOMAINS ALPHA 1, ALPHA 2, ALPHA 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Cellular location (production host): REFOLDED FROM INCLUSION BODIES Production host: ![]() ![]() ![]() |
---|---|
#2: Protein | ![]() Mass: 11793.288 Da / Num. of mol.: 1 / Mutation: Y67C, K91C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Cellular location (production host): REFOLDED FROM INCLUSION BODIES Production host: ![]() ![]() ![]() |
-Protein/peptide , 1 types, 1 molecules C
#3: Protein/peptide | Mass: 1070.280 Da / Num. of mol.: 1 Fragment: RESIDUES 11 - 19 FROM TAX PROTEIN OF HUMAN T LYMPHOTROPIC VIRUS TYPE 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|
-T CELL RECEPTOR ... , 2 types, 2 molecules DE
#4: Protein | Mass: 22488.549 Da / Num. of mol.: 1 / Fragment: EXTRACELLULAR DOMAINS V AND C, RESIDUES 1 - 212 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Cellular location (production host): REFOLDED FROM INCLUSION BODIES Production host: ![]() ![]() ![]() |
---|---|
#5: Protein | Mass: 27360.379 Da / Num. of mol.: 1 / Fragment: EXTRACELLULAR DOMAINS V AND C, RESIDUES 1 - 246 / Mutation: C191A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: V BETA 12.3 [BV13S1] - D BETA 2.1 - J BETA 2.1 - C BETA 2 Organ: PLASMA / Plasmid: PLM1 / Species (production host): Escherichia coli Cellular location (production host): REFOLDED FROM INCLUSION BODIES Production host: ![]() ![]() ![]() |
-Non-polymers , 2 types, 39 molecules ![](data/chem/img/EMC.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#6: Chemical | ![]() #7: Water | ChemComp-HOH / | ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 56 % |
---|---|
Crystal grow![]() | pH: 6.5 Details: CRYSTALLIZED FROM 10% PEG 8000, 100 MM MGACETATE, 50 MM NACACODYLATE, PH 6.5 |
Crystal grow | *PLUS Method: unknown |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM / Detector: CCD / Date: May 5, 1996 / Details: MIRRORS |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.6→12 Å / Num. obs: 29279 / % possible obs: 95 % / Observed criterion σ(I): -3.5 / Redundancy: 3.1 % / Biso Wilson estimate: 48.5 Å2 / Rmerge(I) obs: 0.11 / Rsym value: 0.11 / Net I/σ(I): 7 |
Reflection shell | Resolution: 2.6→2.69 Å / Redundancy: 2.2 % / Rmerge(I) obs: 0.246 / Mean I/σ(I) obs: 3.9 / Rsym value: 0.246 / % possible all: 82.9 |
Reflection shell | *PLUS % possible obs: 82.9 % |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRIES 1HHK, 1BEC CHAIN 1934.4 Resolution: 2.6→6 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 1000000000 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: REFMAC ALSO USED FOR REFINEMENT.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 42.4 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→6 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.6→2.71 Å / Rfactor Rfree error: 0.024 / Total num. of bins used: 8
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | *PLUS Rfactor obs: 0.359 |