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- PDB-1anu: COHESIN-2 DOMAIN OF THE CELLULOSOME FROM CLOSTRIDIUM THERMOCELLUM -
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Open data
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Basic information
Entry | Database: PDB / ID: 1anu | ||||||
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Title | COHESIN-2 DOMAIN OF THE CELLULOSOME FROM CLOSTRIDIUM THERMOCELLUM | ||||||
![]() | COHESIN-2![]() | ||||||
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Function / homology | ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Shimon, L.J.W. / Yaron, S. / Shoham, Y. / Lamed, R. / Morag, E. / Bayer, E.A. / Frolow, F. | ||||||
![]() | ![]() Title: A cohesin domain from Clostridium thermocellum: the crystal structure provides new insights into cellulosome assembly. Authors: Shimon, L.J. / Bayer, E.A. / Morag, E. / Lamed, R. / Yaron, S. / Shoham, Y. / Frolow, F. #1: ![]() Title: Expression, Purification and Subunit-Binding Properties of Cohesins 2 and 3 of the Clostridium Thermocellum Cellulosome Authors: Yaron, S. / Morag, E. / Bayer, E.A. / Lamed, R. / Shoham, Y. #2: ![]() Title: The Cellulosome--A Treasure-Trove for Biotechnology Authors: Bayer, E.A. / Morag, E. / Lamed, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 37.1 KB | Display | ![]() |
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PDB format | ![]() | 25.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 14752.648 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: INCORPORATES CELLULOLYTIC ENZYME INTO THE SCAFFOLDING PROTEIN OF CELLULOSOME Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 51 % | ||||||||||||||||||||||||||||||
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Crystal grow![]() | pH: 8.6 / Details: 28% PEG 6000, 750 MM LICL, 100 MM TRIS-HCL, PH 8.6 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 19 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: May 1, 1996 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.15→30 Å / Num. obs: 8276 / % possible obs: 97.4 % / Observed criterion σ(I): -3 / Redundancy: 5.2 % / Rmerge(I) obs: 0.071 / Rsym value: 0.071 / Net I/σ(I): 31.25 |
Reflection shell | Resolution: 2.15→2.19 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.283 / Mean I/σ(I) obs: 3.5 / Rsym value: 0.283 / % possible all: 84 |
Reflection | *PLUS Num. measured all: 42676 |
Reflection shell | *PLUS % possible obs: 84 % |
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Processing
Software |
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Refinement | Method to determine structure![]() ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.15→30 Å
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Refine LS restraints |
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LS refinement shell | Highest resolution: 2.15 Å / Rfactor Rfree: 0.369 / Rfactor Rwork: 0.351 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.351 |