- PDB-1a8n: SOLUTION STRUCTURE OF A NA+ CATION STABILIZED DNA QUADRUPLEX CONT... -
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Open data
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Basic information
Entry
Database: PDB / ID: 1a8n
Title
SOLUTION STRUCTURE OF A NA+ CATION STABILIZED DNA QUADRUPLEX CONTAINING G.G.G.G AND G.C.G.C TETRADS FORMED BY G-G-G-C REPEATS OBSERVED IN AAV AND HUMAN CHROMOSOME 19, NMR, 8 STRUCTURES
Components
DNA QUADRUPLEX CONTAINING G.G.G.G AND G.C.G.C TETRADS
Keywords
DNA / G-G-G-C REPEAT-CONTAINING DNA QUADRUPLEXES / G.G.G.G TETRADS / G.C.G.C TETRADS / NA+ CATION COORDINATION SITES / DEOXYRIBONUCLEIC ACID
ACCEPTABLE COVALEN GEOMETRY, LOW DISTANCE RESTRAINTS VIOLATIONS AND FAVORABLE NON-BONDED ENERGY
Representative
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Components
#1: DNA chain
DNAQUADRUPLEXCONTAININGG.G.G.GANDG.C.G.CTETRADS
Mass: 3725.412 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: NA+ CATION STABILIZED
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
NOESY
1
2
1
TOCSY
1
3
1
COSY-45
1
4
1
COSY DQF
NMR details
Text: 2D PROTON NOESY EXPERIMENTS IN D2O WERE PERFORMED AT FIVE DIFFERENT MIXING TIMES (50, 100, 150, 200 AND 300 MS). 2D PROTON NOESY EXPERIMENTS IN H2O WERE PERFORMED AT 80 AND 250 MS.
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Sample preparation
Details
Contents: 5 MM NACL, 0.2 MM NA2HPO4
Sample conditions
Ionic strength: 5 mM NACL, 0.2 mM NA2HPO4 / pH: 6.5 / Pressure: 1 atm / Temperature: 293 K
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