Journal: Structure / Year: 1997 Title: The loop E-loop D region of Escherichia coli 5S rRNA: the solution structure reveals an unusual loop that may be important for binding ribosomal proteins. Authors: Dallas, A. / Moore, P.B.
NO NOE, DIHEDRAL VIOLATIONS, LOW OVERALL ENERGY STRUCTURES, GOOD LOCAL GEOMETRY
Representative
-
Components
#1: RNA chain
5SRRNALOOPD/LOOPE
Mass: 13299.919 Da / Num. of mol.: 1 / Fragment: LOOP D/LOOP E ARM Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Cellular location: CYTOPLASM / Plasmid: PAD3 / Production host: Escherichia coli (E. coli) / Strain (production host): JM101
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
-
Sample preparation
Sample conditions
Conditions-ID
pH
Temperature (K)
1
7.2
283K
2
5.5
283K
Crystal grow
*PLUS
Method: other / Details: NMR
-
NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Varian UNITY
Varian
UNITY
500
1
GE UNITYPLUS
GE
UNITYPLUS
600
2
GE OMEGA
GE
OMEGA
600
3
-
Processing
Software
Name
Classification
X-PLOR
modelbuilding
X-PLOR
refinement
X-PLOR
phasing
NMR software
Name
Developer
Classification
X-PLOR
BRUNGER
refinement
X-PLOR
structuresolution
Refinement
Method: TORSION ANGLE MOLECULAR DYNAMICS / Software ordinal: 1 / Details: FOR REFINEMENT DETAILS, SEE STRUCTURE,5,P.1639
NMR ensemble
Conformer selection criteria: NO NOE, DIHEDRAL VIOLATIONS, LOW OVERALL ENERGY STRUCTURES, GOOD LOCAL GEOMETRY Conformers calculated total number: 60 / Conformers submitted total number: 9
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