- EMDB-8425: Chikungunya virus-like particle inserted with circumsporozoite pr... -
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Basic information
Entry
Database: EMDB / ID: EMD-8425
Title
Chikungunya virus-like particle inserted with circumsporozoite protein peptides of Plasmodium falciparum in complex with Fab fragment of neutralizing antibody DG2
Map data
Chikungunya virus-like particle inserted with circumsporozoite protein peptides of Plasmodium falciparum in complex with Fab fragment of neutralizing antibody DG2
Sample
Complex: Chikungunya virus / Fab complex
Virus: Chikungunya virus strain Senegal 37997
Organelle or cellular component: Fab fragment of neutralizing antibody DG2
Journal: Clin Vaccine Immunol / Year: 2017 Title: Development of a Novel Virus-Like Particle Vaccine Platform That Mimics the Immature Form of Alphavirus. Authors: Akane Urakami / Atsuko Sakurai / Momoko Ishikawa / Moh Lan Yap / Yevel Flores-Garcia / Yasunari Haseda / Taiki Aoshi / Fidel P Zavala / Michael G Rossmann / Sachiko Kuno / Ryuji Ueno / Wataru Akahata / Abstract: Virus-like particles (VLPs) are noninfectious multiprotein structures that are engineered to self-assemble from viral structural proteins. Here, we developed a novel VLP-based vaccine platform ...Virus-like particles (VLPs) are noninfectious multiprotein structures that are engineered to self-assemble from viral structural proteins. Here, we developed a novel VLP-based vaccine platform utilizing VLPs from the chikungunya virus. We identified two regions within the envelope protein, a structural component of chikungunya, where foreign antigens can be inserted without compromising VLP structure. Our VLP displays 480 copious copies of an inserted antigen on the VLP surface in a highly symmetric manner and is thus capable of inducing strong immune responses against any inserted antigen. Furthermore, by mimicking the structure of the immature form of the virus, we altered our VLP's dynamics and enhanced its immunogenicity. We used the circumsporozoite protein (CSP) of the malaria parasite as an antigen and demonstrated that our VLP-based vaccine elicits strong immune responses against CSP in animals. The sera from immunized monkeys protected mice from malaria infection. Likewise, mice vaccinated with CSP-containing VLPs were protected from an infectious sporozoite challenge. Hence, our uniquely engineered VLP platform can serve as a blueprint for the development of vaccines against other pathogens and diseases.
History
Deposition
Oct 6, 2016
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Header (metadata) release
Oct 19, 2016
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Map release
Feb 21, 2018
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Update
Apr 24, 2019
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Current status
Apr 24, 2019
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Download / File: emd_8425.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation
Chikungunya virus-like particle inserted with circumsporozoite protein peptides of Plasmodium falciparum in complex with Fab fragment of neutralizing antibody DG2
Voxel size
X=Y=Z: 2.6 Å
Density
Contour Level
By AUTHOR: 0.63 / Movie #1: 0.63
Minimum - Maximum
-3.8666894 - 3.2762864
Average (Standard dev.)
0.0019805962 (±0.3326793)
Symmetry
Space group: 1
Details
EMDB XML:
Map geometry
Axis order
X
Y
Z
Origin
-192
-192
-192
Dimensions
384
384
384
Spacing
384
384
384
Cell
A=B=C: 998.39996 Å α=β=γ: 90.0 °
CCP4 map header:
mode
Image stored as Reals
Å/pix. X/Y/Z
2.6
2.6
2.6
M x/y/z
384
384
384
origin x/y/z
0.000
0.000
0.000
length x/y/z
998.400
998.400
998.400
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
-192
-192
-192
NC/NR/NS
384
384
384
D min/max/mean
-3.867
3.276
0.002
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Supplemental data
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Sample components
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Entire : Chikungunya virus / Fab complex
Entire
Name: Chikungunya virus / Fab complex
Components
Complex: Chikungunya virus / Fab complex
Virus: Chikungunya virus strain Senegal 37997
Organelle or cellular component: Fab fragment of neutralizing antibody DG2
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