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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Artificial membrane protein TMHC4_R (ROCKET) | |||||||||
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![]() | Synthetic construct / ![]() ![]() | |||||||||
Biological species | synthetic construct (others) | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Abramsson ML / Anden O / Howard RJ / Lindahl E / Landreh M | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Engineering cardiolipin binding to an artificial membrane protein Authors: Abramsson M / Corey RA / Skerle J / Persson L / Anden O / Oluwole AO / Howard RJ / Lindahl E / Robinson CV / Strisovsky K / Marklund EG / Drew D / Stansfeld PJ / Landreh M | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 31.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.6 KB 14.6 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 18.3 KB | Display | ![]() |
Images | ![]() | 78.3 KB | ||
Masks | ![]() | 512 MB | ![]() | |
Filedesc metadata | ![]() | 4.9 KB | ||
Others | ![]() ![]() | 403.8 MB 403.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
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Voxel size | X=Y=Z: 0.5076 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: #1
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_50106_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Artificial membrane protein TMHC4_R (ROCKET)
Entire | Name: Artificial membrane protein TMHC4_R (ROCKET) |
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Components |
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-Supramolecule #1: Artificial membrane protein TMHC4_R (ROCKET)
Supramolecule | Name: Artificial membrane protein TMHC4_R (ROCKET) / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: synthetic construct (others) |
Molecular weight | Theoretical: 100.40 kDa/nm |
-Macromolecule #1: TMHC4_R (ROCKET)
Macromolecule | Name: TMHC4_R (ROCKET) / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: synthetic construct (others) |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MSKDTEDSRK IWRTIMLLLV FAILLSAIIW YQITTNPDTS QIATLLSMQL LLIALMLVVI ALLLSRQTEQ VAESIRRDVS ALAYVMLGLL LSLLNRLSLA AEAYKKAIEL DPNDALAWLL LGSVLEKLKR LDEAAEAYKK AIELKPNDAS AWKELGKVLE KLGRLDEAAE ...String: MSKDTEDSRK IWRTIMLLLV FAILLSAIIW YQITTNPDTS QIATLLSMQL LLIALMLVVI ALLLSRQTEQ VAESIRRDVS ALAYVMLGLL LSLLNRLSLA AEAYKKAIEL DPNDALAWLL LGSVLEKLKR LDEAAEAYKK AIELKPNDAS AWKELGKVLE KLGRLDEAAE AYKKAIELDP EDAEAWKELG KVLEKLGRLD EAAEAYKKAI ELDPNDLEHH HHHH |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | 3D array |
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Sample preparation
Buffer | pH: 8 |
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 60.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |