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- EMDB-50071: Cryo-EM structure of the icosahedral lumazine synthase from Vicia... -
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Open data
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Basic information
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Title | Cryo-EM structure of the icosahedral lumazine synthase from Vicia faba. | |||||||||
![]() | Sharpened map (from RELION Postprocess) | |||||||||
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![]() | icosahedral enzyme / ![]() | |||||||||
Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Chee M / Trapani S / Hoh F / Lai Kee Him J / Yvon M / Blanc S / Bron P | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of the icosahedral lumazine synthase from Vicia faba. Authors: Chee M / Trapani S / Hoh F / Lai Kee Him J / Yvon M / Blanc S / Bron P | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 117 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 16.7 KB 16.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 11.3 KB | Display | ![]() |
Images | ![]() | 231.7 KB | ||
Masks | ![]() | 125 MB | ![]() | |
Filedesc metadata | ![]() | 5.4 KB | ||
Others | ![]() ![]() ![]() | 95 MB 95.8 MB 95.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 9ez8MC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | Sharpened map (from RELION Postprocess) | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.2625 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Additional map: Unsharpened map
File | emd_50071_additional_1.map | ||||||||||||
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Annotation | Unsharpened map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_50071_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_50071_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Icosahedral 60-mer
Entire | Name: Icosahedral 60-mer |
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Components |
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-Supramolecule #1: Icosahedral 60-mer
Supramolecule | Name: Icosahedral 60-mer / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 1.45 MDa |
-Macromolecule #1: 6,7-dimethyl-8-ribityllumazine synthase
Macromolecule | Name: 6,7-dimethyl-8-ribityllumazine synthase / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: ![]() |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 16.505764 KDa |
Sequence | String: AVRHLTGSVT RTQGLRFAVV VARFNEIITR PLLEGALGTF KNYSVQDEDI DVVWVPGCFE IGAVATRLGK SGKYHAIICI GAVIRGDTT HYDAVANSAA SGVLSAGLNS GVPCIFGVLT CEDMDQAINR AGGKSGNKGA EAALTAIEMA SLFEHHLQ UniProtKB: ![]() |
-Experimental details
-Structure determination
Method | ![]() |
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![]() | ![]() |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TALOS ARCTICA |
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Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 7.34 e/Å2 |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Initial model | Chain - Source name: Other / Chain - Initial model type: other / Details: poly-Ala model manually built using Coot |
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Output model | ![]() PDB-9ez8: |