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Yorodumi- EMDB-43313: Constituent EM map: Focused refinement Ry3&4 of mouse RyR1 (EGTA-... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-43313 | |||||||||
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Title | Constituent EM map: Focused refinement Ry3&4 of mouse RyR1 (EGTA-only dataset) | |||||||||
Map data | Constituent EM map: Focused refinement Ry3&4 of mouse RyR1 (EGTA-only dataset) | |||||||||
Sample |
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Keywords | Calcium / Ion Channel / MEMBRANE PROTEIN | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.55 Å | |||||||||
Authors | Weninger G / Marks AR | |||||||||
Funding support | United States, 1 items
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Citation | Journal: To Be Published Title: Structural insights into the regulation of RyR1 by S100A1. Authors: Weninger G | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_43313.map.gz | 254.6 MB | EMDB map data format | |
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Header (meta data) | emd-43313-v30.xml emd-43313.xml | 14.1 KB 14.1 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_43313_fsc.xml | 16.9 KB | Display | FSC data file |
Images | emd_43313.png | 40.2 KB | ||
Filedesc metadata | emd-43313.cif.gz | 3.9 KB | ||
Others | emd_43313_half_map_1.map.gz emd_43313_half_map_2.map.gz | 475.5 MB 475.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-43313 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-43313 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_43313.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | Constituent EM map: Focused refinement Ry3&4 of mouse RyR1 (EGTA-only dataset) | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_43313_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_43313_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Complex of RyR1 with Calstabin-1 (EGTA condition)
Entire | Name: Complex of RyR1 with Calstabin-1 (EGTA condition) |
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Components |
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-Supramolecule #1: Complex of RyR1 with Calstabin-1 (EGTA condition)
Supramolecule | Name: Complex of RyR1 with Calstabin-1 (EGTA condition) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: Mus musculus (house mouse) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 8.5 mg/mL | |||||||||||||||||||||
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Buffer | pH: 7.4 Component:
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Grid | Model: Quantifoil R0.6/1 / Material: GOLD / Mesh: 300 | |||||||||||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | C2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 1.2 µm / Nominal defocus min: 0.5 µm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 12147 / Average electron dose: 58.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |