+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-41844 | |||||||||
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Title | Cryo-EM structure of C.crescentus bNY30a pilus complex | |||||||||
Map data | main | |||||||||
Sample |
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Keywords | Complex / PROTEIN FIBRIL | |||||||||
Function / homology | Flp/Fap pilin component / Flp/Fap pilin component / membrane / Flp family type IVb pilin Function and homology information | |||||||||
Biological species | Caulobacter vibrioides (bacteria) | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 0.86 Å | |||||||||
Authors | Wang Y / Zhang J | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Sci Adv / Year: 2024 Title: Structural mechanisms of Tad pilus assembly and its interaction with an RNA virus. Authors: Yuhang Wang / Matthew Theodore / Zhongliang Xing / Utkarsh Narsaria / Zihao Yu / Lanying Zeng / Junjie Zhang / Abstract: Tad (tight adherence) pili, part of the type IV pili family, are crucial for mechanosensing, surface adherence, bacteriophage (phage) adsorption, and cell-cycle regulation. Unlike other type IV ... Tad (tight adherence) pili, part of the type IV pili family, are crucial for mechanosensing, surface adherence, bacteriophage (phage) adsorption, and cell-cycle regulation. Unlike other type IV pilins, Tad pilins lack the typical globular β sheet domain responsible for pilus assembly and phage binding. The mechanisms of Tad pilus assembly and its interaction with phage ΦCb5 have been elusive. Using cryo-electron microscopy, we unveiled the Tad pilus assembly mechanism, featuring a unique network of hydrogen bonds at its core. We then identified the Tad pilus binding to the ΦCb5 maturation protein (Mat) through its β region. Notably, the amino terminus of ΦCb5 Mat is exposed outside the capsid and phage/pilus interface, enabling the attachment of fluorescent and affinity tags. These engineered ΦCb5 virions can be efficiently assembled and purified in , maintaining infectivity against , which presents promising applications, including RNA delivery and phage display. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_41844.map.gz | 69.7 MB | EMDB map data format | |
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Header (meta data) | emd-41844-v30.xml emd-41844.xml | 14 KB 14 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_41844_fsc.xml | 10.9 KB | Display | FSC data file |
Images | emd_41844.png | 33.4 KB | ||
Filedesc metadata | emd-41844.cif.gz | 5 KB | ||
Others | emd_41844_half_map_1.map.gz emd_41844_half_map_2.map.gz | 129.7 MB 129.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-41844 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-41844 | HTTPS FTP |
-Related structure data
Related structure data | 8u2bMC 8ucrC 8uejC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_41844.map.gz / Format: CCP4 / Size: 139.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | main | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.86 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: b
File | emd_41844_half_map_1.map | ||||||||||||
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Annotation | b | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: a
File | emd_41844_half_map_2.map | ||||||||||||
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Annotation | a | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Caulobacter crescentus pili
Entire | Name: Caulobacter crescentus pili |
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Components |
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-Supramolecule #1: Caulobacter crescentus pili
Supramolecule | Name: Caulobacter crescentus pili / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all / Details: Caulobacter crescentus pili in buffer |
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Source (natural) | Organism: Caulobacter vibrioides (bacteria) |
Molecular weight | Theoretical: 170 KDa |
-Macromolecule #1: Flp family type IVb pilin
Macromolecule | Name: Flp family type IVb pilin / type: protein_or_peptide / ID: 1 / Number of copies: 37 / Enantiomer: LEVO |
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Source (natural) | Organism: Caulobacter vibrioides (bacteria) |
Molecular weight | Theoretical: 4.37914 KDa |
Sequence | String: ATAIEYGLIV ALIAVVIVTA VTTLGTKLNL AFTKAGTAVS TAAGT UniProtKB: Flp family type IVb pilin |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | helical reconstruction |
Aggregation state | filament |
-Sample preparation
Buffer | pH: 7.5 Component:
Details: 20mM tris, 2mM MgCl2 | |||||||||
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Grid | Model: C-flat-2/1 / Material: COPPER | |||||||||
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 25.0 µm / Nominal defocus min: 5.0 µm |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |