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- EMDB-4050: 70S ribosome from Staphylococcus aureus -

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Basic information

Entry
Database: EMDB / ID: 4050
Title70S ribosome from Staphylococcus aureus
Sample70S ribosome from Staphylococcus aureus
SourceStaphylococcus aureus (strain nctc 8325) / bacteria
Map data
Methodsingle particle reconstruction, at 3.8 A resolution
AuthorsKhusainov I / Vicens Q
CitationNucleic Acids Res., 2016, 44, 10491-10504

Nucleic Acids Res., 2016, 44, 10491-10504 StrPapers
Structure of the 70S ribosome from human pathogen Staphylococcus aureus.
Iskander Khusainov / Quentin Vicens / Anthony Bochler / François Grosse / Alexander Myasnikov / Jean-François Ménétret / Johana Chicher / Stefano Marzi / Pascale Romby / Gulnara Yusupova / Marat Yusupov / Yaser Hashem

DateDeposition: Jul 13, 2016 / Header (metadata) release: Dec 28, 2016 / Map release: Dec 28, 2016 / Last update: Jul 13, 2016

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.085
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view colored by height
  • Surface level: 0.085
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view with fitted model
  • Atomic models: : PDB-5li0
  • Surface level: 0.085
  • Imaged by UCSF CHIMERA
  • Download
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Supplemental images

Downloads & links

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Map

Fileemd_4050.map.gz (map file in CCP4 format, 146378 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
332 pix
1.1 A/pix
= 365.2 A
332 pix
1.1 A/pix
= 365.2 A
332 pix
1.1 A/pix
= 365.2 A

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 1.1 A
Density
Contour Level:0.095 (by author), 0.085 (movie #1):
Minimum - Maximum-0.23831725 - 0.44680446
Average (Standard dev.)0.0016471746 (0.02667443)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions332332332
Origin000
Limit331331331
Spacing332332332
CellA=B=C: 365.2 A
Alpha=beta=gamma: 90 deg.

CCP4 map header:

modeImage stored as Reals
A/pix X/Y/Z1.11.11.1
M x/y/z332332332
origin x/y/z0.0230.0230.023
length x/y/z365.200365.200365.200
alpha/beta/gamma90.00090.00090.000
start NX/NY/NZ
NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS332332332
D min/max/mean-0.2380.4470.002

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Supplemental data

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Sample components

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Entire 70S ribosome from Staphylococcus aureus

EntireName: 70S ribosome from Staphylococcus aureus / Number of components: 55

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Component #1: protein, 70S ribosome from Staphylococcus aureus

ProteinName: 70S ribosome from Staphylococcus aureus / Recombinant expression: No
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #2: nucleic-acid, 16S ribosomal RNA

Nucleic-acidName: 16S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GGAGAGUUUG AUCCUGGCUC AGGAUGAACG CUGGCGGCGU GCCUAAUACA UGCAAGUCGA GCGAACGGAC GAGAAGCUUG CUUCUCUGAU GUUAGCGGCG GACGGGUGAG UAACACGUGG AUAACCUACC UAUAAGACUG GGAUAACUUC GGGAAACCGU AGCUAAUACC GGAUAAUAUU UUGAACCGCA UGGUUCAAAA GUGAAAGACG GUCUUGCUGU CACUUAUAGA UGGAUCCGCG CUGCAUUAGC UAGUUGGUAA GGUAACGGCU UACCAAGGCA ACGAUGCAUA GCCGACCUGA GAGGGUGAUC GGCCACACUG GAACUGAGAC ACGGUCCAGA CUCCUACGGG AGGCAGCAGU AGGGAAUCUU CCGCAAUGGG CGAAAGCCUG ACGGAGCAAC GCCGCGUGAG UGAUGAAGGU CUUCGGAUCG UAAAACUCUG UUAUUAGGGA AGAACAUAUG UGUAAGUAAC UGUGCACAUC UUGACGGUAC CUAAUCAGAA AGCCACGGCU AACUACGUGC CAGCAGCCGC GGUAAUACGU AGGUGGCAAG CGUUAUCCGG AAUUAUUGGG CGUAAAGCGC GCGUAGGCGG UUUUUUAAGU CUGAUGUGAA AGCCCACGGC UCAACCGUGG AGGGUCAUUG GAAACUGGAA AACUUGAGUG CAGAAGAGGA AAGUGGAAUU CCAUGUGUAG CGGUGAAAUG CGCAGAGAUA UGGAGGAACA CCAGUGGCGA AGGCGACUUU CUGGUCUGUA ACUGACGCUG AUGUGCGAAA GCGUGGGGAU CAAACAGGAU UAGAUACCCU GGUAGUCCAC GCCGUAAACG AUGAGUGCUA AGUGUUAGGG GGUUUCCCGC CCCUUAGUGC UGCAGCUAAC GCAUUAAGCA CUCCGCCUGG GGAGUACGAC CGCAAGGUUG AAACUCAAAG GAAUUGACGG GGACCCGCAC AAGCGGUGGA GCAUGUGGUU UAAUUCGAAG CAACGCGAAG AACCUUACCA AAUCUUGACA UCCUUUGACA ACUCUAGAGA UAGAGCCUUC CCCUUCGGGG GACAAAGUGA CAGGUGGUGC AUGGUUGUCG UCAGCUCGUG UCGUGAGAUG UUGGGUUAAG UCCCGCAACG AGCGCAACCC UUAAGCUUAG UUGCCAUCAU UAAGUUGGGC ACUCUAAGUU GACUGCCGGU GACAAACCGG AGGAAGGUGG GGAUGACGUC AAAUCAUCAU GCCCCUUAUG AUUUGGGCUA CACACGUGCU ACAAUGGACA AUACAAAGGG CAGCGAAACC GCGAGGUCAA GCAAAUCCCA UAAAGUUGUU CUCAGUUCGG AUUGUAGUCU GCAACUCGAC UACAUGAAGC UGGAAUCGCU AGUAAUCGUA GAUCAGCAUG CUACGGUGAA UACGUUCCCG GGUCUUGUAC ACACCGCCCG UCACACCACG AGAGUUUGUA ACACCCGAAG CCGGUGGAGU AACCUUUUAG GAGCUAGCCG UCGAAGGUGG GACAAAUGAU UGGGGUGAAG UCGUAACAAG GUAGCCGUAU CGGAAGGUGC GGCUGGAUCA CCUCCUU
MassTheoretical: 500.464406 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #3: protein, 30S ribosomal protein S2

ProteinName: 30S ribosomal protein S2 / Recombinant expression: No
MassTheoretical: 29.136369 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #4: protein, 30S ribosomal protein S3

ProteinName: 30S ribosomal protein S3 / Recombinant expression: No
MassTheoretical: 24.143867 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #5: protein, 30S ribosomal protein S4

ProteinName: 30S ribosomal protein S4 / Recombinant expression: No
MassTheoretical: 22.920221 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #6: protein, 30S ribosomal protein S5

ProteinName: 30S ribosomal protein S5 / Recombinant expression: No
MassTheoretical: 17.639314 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #7: protein, 30S ribosomal protein S6

ProteinName: 30S ribosomal protein S6 / Recombinant expression: No
MassTheoretical: 11.368879 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #8: protein, 30S ribosomal protein S7

ProteinName: 30S ribosomal protein S7 / Recombinant expression: No
MassTheoretical: 17.167736 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #9: protein, 30S ribosomal protein S8

ProteinName: 30S ribosomal protein S8 / Recombinant expression: No
MassTheoretical: 14.723118 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #10: protein, 30S ribosomal protein S9

ProteinName: 30S ribosomal protein S9 / Recombinant expression: No
MassTheoretical: 14.44045 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #11: protein, 30S ribosomal protein S10

ProteinName: 30S ribosomal protein S10 / Recombinant expression: No
MassTheoretical: 11.60052 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #12: protein, 30S ribosomal protein S11

ProteinName: 30S ribosomal protein S11 / Recombinant expression: No
MassTheoretical: 12.562294 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #13: protein, 30S ribosomal protein S12

ProteinName: 30S ribosomal protein S12 / Recombinant expression: No
MassTheoretical: 15.131598 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #14: protein, 30S ribosomal protein S13

ProteinName: 30S ribosomal protein S13 / Recombinant expression: No
MassTheoretical: 13.487543 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #15: protein, 30S ribosomal protein S14 type Z

ProteinName: 30S ribosomal protein S14 type Z / Recombinant expression: No
MassTheoretical: 7.186573 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #16: protein, 30S ribosomal protein S15

ProteinName: 30S ribosomal protein S15 / Recombinant expression: No
MassTheoretical: 10.503135 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #17: protein, 30S ribosomal protein S16

ProteinName: 30S ribosomal protein S16 / Recombinant expression: No
MassTheoretical: 10.12269 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #18: protein, 30S ribosomal protein S17

ProteinName: 30S ribosomal protein S17 / Recombinant expression: No
MassTheoretical: 10.065691 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #19: protein, 30S ribosomal protein S18

ProteinName: 30S ribosomal protein S18 / Recombinant expression: No
MassTheoretical: 8.418959 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #20: protein, 30S ribosomal protein S19

ProteinName: 30S ribosomal protein S19 / Recombinant expression: No
MassTheoretical: 9.605988 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #21: protein, 30S ribosomal protein S20

ProteinName: 30S ribosomal protein S20 / Recombinant expression: No
MassTheoretical: 8.708018 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #22: nucleic-acid, 23S ribosomal RNA

Nucleic-acidName: 23S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
UUAAGUUAUU AAGGGCGCAC GGUGGAUGCC UUGGCACUAG AAGCCGAUGA AGGACGUUAC UAACGACGAU AUGCUUUGGG GAGCUGUAAG UAAGCUUUGA UCCAGAGAUU UCCGAAUGGG GAAACCCAGC AUGAGUUAUG UCAUGUUAUC GAUAUGUGAA UACAUAGCAU AUCAGAAGGC ACACCCGGAG AACUGAAACA UCUUAGUACC CGGAGGAAGA GAAAGAAAAU UCGAUUCCCU UAGUAGCGGC GAGCGAAAUG GGAAGAGCCC AAACCAACAA GCUUGCUUGU UGGGGUUGUA GGACACUCUA UACGGAGUUA CAAAGGACGA CAUUAGACGA AUCAUCUGGA AAGAUGAAUC AAAGAAGGUA AUAAUCCUGU AGUCGAAAAU GUUGUCUCUC UUGAGUGGAU CCUGAGUACG ACGGAGCACG UGAAAUUCCG UCGGAAUCUG GGAGGACCAU CUCCUAAGGC UAAAUACUCU CUAGUGACCG AUAGUGAACC AGUACCGUGA GGGAAAGGUG AAAAGCACCC CGGAAGGGGA GUGAAAUAGA ACCUGAAACC GUGUGCUUAC AAGUAGUCAG AGCCCGUUAA UGGGUGAUGG CGUGCCUUUU GUAGAAUGAA CCGGCGAGUU ACGAUUUGAU GCAAGGUUAA GCAGUAAAUG UGGAGCCGUA GCGAAAGCGA GUCUGAAUAG GGCGUUUAGU AUUUGGUCGU AGACCCGAAA CCAGGUGAUC UACCCUUGGU CAGGUUGAAG UUCAGGUAAC ACUGAAUGGA GGACCGAACC GACUUACGUU GAAAAGUGAG CGGAUGAACU GAGGGUAGCG GAGAAAUUCC AAUCGAACCU GGAGAUAGCU GGUUCUCUCC GAAAUAGCUU UAGGGCUAGC CUCAAGUGAU GAUUAUUGGA GGUAGAGCAC UGUUUGGACG AGGGGCCCCU CUCGGGUUAC CGAAUUCAGA CAAACUCCGA AUGCCAAUUA AUUUAACUUG GGAGUCAGAA CAUGGGUGAU AAGGUCCGUG UUCGAAAGGG AAACAGCCCA GACCACCAGC UAAGGUCCCA AAAUAUAUGU UAAGUGGAAA AGGAUGUGGC GUUGCCCAGA CAACUAGGAU GUUGGCUUAG AAGCAGCCAU CAUUUAAAGA GUGCGUAAUA GCUCACUAGU CGAGUGACAC UGCGCCGAAA AUGUACCGGG GCUAAACAUA UUACCGAAGC UGUGGAUUGU CCUUUGGACA AUGGUAGGAG AGCGUUCUAA GGGCGUUGAA GCAUGAUCGU AAGGACAUGU GGAGCGCUUA GAAGUGAGAA UGCCGGUGUG AGUCGCGAAA GACGGGUGAG AAUCCCGUCC ACCGAUUGAC UAAGGUUUCC AGAGGAAGGC UCGUCCGCUC UGGGUUAGUC GGGUCCUAAG CUGAGGCCGA CAGGCGUAGG CGAUGGAUAA CAGGUUGAUA UUCCUGUACC ACCUAUAAUC GUUUUAAUCG AUGGGGGGAC GCAGUAGGAU AGGCGAAGCG UGCGAUUGGA UUGCACGUCU AAGCAGUAAG GCUGAGUAUU AGGCAAAUCC GGUACUCGUU AAGGCUGAGC UGUGAUGGGG AGAAGACAUU GAGUCUUCGA GUCGUUGAUU UCACACUGCC GAGAAAAGCC UCUAGAUAGA AAAUAGGUGC CCGUACCGCA AACCGACACA GGUAGUCAAG AUGAGAAUUC UAAGGUGAGC GAGCGAACUC UCGUUAAGGA ACUCGGCAAA AUGACCCCGU AACUUCGGGA GAAGGGGUGC UCUUUAGGGU UAACGCCCAG AAGAGCCGCA GUGAAUAGGC CCAAGCGACU GUUUAUCAAA AACACAGGUC UCUGCUAAAC CGUAAGGUGA UGUAUAGGGG CUGACGCCUG CCCGGUGCUG GAAGGUUAAG AGGAGUGGUU AGCUUCUGCG AAGCUACGAA UCGAAGCCCC AGUAAACGGC GGCCGUAACU AUAACGGUCC UAAGGUAGCG AAAUUCCUUG UCGGGUAAGU UCCGACCCGC ACGAAAGGCG UAACGAUUUG GGCACUGUCU CAACGAGAGA CUCGGUGAAA UCAUAGUACC UGUGAAGAUG CAGGUUACCC GCGACAGGAC GGAAAGACCC CGUGGAGCUU UACUGUAGCC UGAUAUUGAA AUUCGGCACA GCUUGUACAG GAUAGGUAGG AGCCUUUGAA ACGUGAGCGC UAGCUUACGU GGAGGCGCUG GUGGGAUACU ACCCUAGCUG UGUUGGCUUU CUAACCCGCA CCACUUAUCG UGGUGGGAGA CAGUGUCAGG CGGGCAGUUU GACUGGGGCG GUCGCCUCCU AAAAGGUAAC GGAGGCGCUC AAAGGUUCCC UCAGAAUGGU UGGAAAUCAU UCAUAGAGUG UAAAGGCAUA AGGGAGCUUG ACUGCGAGAC CUACAAGUCG AGCAGGGUCG AAAGACGGAC UUAGUGAUCC GGUGGUUCCG CAUGGAAGGG CCAUCGCUCA ACGGAUAAAA GCUACCCCGG GGAUAACAGG CUUAUCUCCC CCAAGAGUUC ACAUCGACGG GGAGGUUUGG CACCUCGAUG UCGGCUCAUC GCAUCCUGGG GCUGUAGUCG GUCCCAAGGG UUGGGCUGUU CGCCCAUUAA AGCGGUACGC GAGCUGGGUU CAGAACGUCG UGAGACAGUU CGGUCCCUAU CCGUCGUGGG CGUAGGAAAU UUGAGAGGAG CUGUCCUUAG UACGAGAGGA CCGGGAUGGA CAUACCUCUG GUGUACCAGU UGUCGUGCCA ACGGCAUAGC UGGGUAGCUA UGUGUGGACG GGAUAAGUGC UGAAAGCAUC UAAGCAUGAA GCCCCCCUCA AGAUGAGAUU UCCCAACUUC GGUUAUAAGA UCCCUCAAAG AUGAUGAGGU UAAUAGGUUC GAGGUGGAAG CAUGGUGACA UGUGGAGCUG ACGAAUACUA AUCGAUCGAA GACUUAAU
MassTheoretical: 945.058562 kDa

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Component #23: nucleic-acid, 5S ribosomal RNA

Nucleic-acidName: 5S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
UCUGGUGACU AUAGCAAGGA GGUCACACCU GUUCCCAUGC CGAACACAGA AGUUAAGGUC UUUAGCGACG AUGGUAGCCA ACUUACGUUC CGCUAGAGUA GAACGUUGCC AGGC
MassTheoretical: 36.692801 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #24: protein, 50S ribosomal protein L2

ProteinName: 50S ribosomal protein L2 / Recombinant expression: No
MassTheoretical: 29.956789 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #25: protein, 50S ribosomal protein L3

ProteinName: 50S ribosomal protein L3 / Recombinant expression: No
MassTheoretical: 23.499877 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #26: protein, 50S ribosomal protein L4

ProteinName: 50S ribosomal protein L4 / Recombinant expression: No
MassTheoretical: 21.666725 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #27: protein, 50S ribosomal protein L5

ProteinName: 50S ribosomal protein L5 / Recombinant expression: No
MassTheoretical: 18.729721 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #28: protein, 50S ribosomal protein L6

ProteinName: 50S ribosomal protein L6 / Recombinant expression: No
MassTheoretical: 18.296711 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #29: protein, 50S ribosomal protein L13

ProteinName: 50S ribosomal protein L13 / Recombinant expression: No
MassTheoretical: 16.359427 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #30: protein, 50S ribosomal protein L14

ProteinName: 50S ribosomal protein L14 / Recombinant expression: No
MassTheoretical: 13.157342 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #31: protein, 50S ribosomal protein L15

ProteinName: 50S ribosomal protein L15 / Recombinant expression: No
MassTheoretical: 14.193304 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #32: protein, 50S ribosomal protein L16

ProteinName: 50S ribosomal protein L16 / Recombinant expression: No
MassTheoretical: 15.943755 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #33: protein, 50S ribosomal protein L17

ProteinName: 50S ribosomal protein L17 / Recombinant expression: No
MassTheoretical: 13.42035 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #34: protein, 50S ribosomal protein L18

ProteinName: 50S ribosomal protein L18 / Recombinant expression: No
MassTheoretical: 13.124093 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #35: protein, 50S ribosomal protein L19

ProteinName: 50S ribosomal protein L19 / Recombinant expression: No
MassTheoretical: 12.594825 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #36: protein, 50S ribosomal protein L20

ProteinName: 50S ribosomal protein L20 / Recombinant expression: No
MassTheoretical: 13.459919 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #37: protein, 50S ribosomal protein L21

ProteinName: 50S ribosomal protein L21 / Recombinant expression: No
MassTheoretical: 11.354081 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #38: protein, 50S ribosomal protein L22

ProteinName: 50S ribosomal protein L22 / Recombinant expression: No
MassTheoretical: 12.328358 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #39: protein, 50S ribosomal protein L23

ProteinName: 50S ribosomal protein L23 / Recombinant expression: No
MassTheoretical: 10.3801 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #40: protein, 50S ribosomal protein L24

ProteinName: 50S ribosomal protein L24 / Recombinant expression: No
MassTheoretical: 10.976878 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #41: protein, 50S ribosomal protein L25

ProteinName: 50S ribosomal protein L25 / Recombinant expression: No
MassTheoretical: 10.522318 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #42: protein, 50S ribosomal protein L27

ProteinName: 50S ribosomal protein L27 / Recombinant expression: No
MassTheoretical: 8.911099 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #43: protein, 50S ribosomal protein L28

ProteinName: 50S ribosomal protein L28 / Recombinant expression: No
MassTheoretical: 5.312262 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #44: protein, 50S ribosomal protein L29

ProteinName: 50S ribosomal protein L29 / Recombinant expression: No
MassTheoretical: 7.660762 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #45: protein, 50S ribosomal protein L30

ProteinName: 50S ribosomal protein L30 / Recombinant expression: No
MassTheoretical: 6.305308 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #46: protein, 50S ribosomal protein L31 type B

ProteinName: 50S ribosomal protein L31 type B / Recombinant expression: No
MassTheoretical: 9.737912 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #47: protein, 50S ribosomal protein L32

ProteinName: 50S ribosomal protein L32 / Recombinant expression: No
MassTheoretical: 6.397427 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #48: protein, 50S ribosomal protein L33 2

ProteinName: 50S ribosomal protein L33 2 / Recombinant expression: No
MassTheoretical: 4.711371 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #49: protein, 50S ribosomal protein L34

ProteinName: 50S ribosomal protein L34 / Recombinant expression: No
MassTheoretical: 5.323446 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #50: protein, 50S ribosomal protein L35

ProteinName: 50S ribosomal protein L35 / Recombinant expression: No
MassTheoretical: 6.985402 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #51: protein, 50S ribosomal protein L36

ProteinName: 50S ribosomal protein L36 / Recombinant expression: No
MassTheoretical: 4.318422 kDa
SourceSpecies: Staphylococcus aureus (strain nctc 8325) / bacteria

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Component #52: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 31 / Recombinant expression: No
MassTheoretical: 2.430505 MDa

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Component #53: ligand, OXYGEN ATOM

LigandName: OXYGEN ATOM / Number of Copies: 9 / Recombinant expression: No
MassTheoretical: 1.599905 MDa

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Component #54: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

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Component #55: ligand, water

LigandName: water / Number of Copies: 162 / Recombinant expression: No
MassTheoretical: 1.801505 MDa

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Experimental details

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Sample preparation

Specimen stateparticle
Sample solutionBuffer solution: 5 mM Hepes-KOH pH 7.5 50 mM KCl 10 mM NH4Cl 10 mM Mg(OAc)2 1 mM DTT
pH: 7.5
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 277 K / Humidity: 100 % / Details: blot force 5, blot waiting time 30 s

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Electron microscopy imaging

ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 60 e/A2 / Illumination mode: FLOOD BEAM
LensImaging mode: DARK FIELD
Specimen HolderModel: OTHER
CameraDetector: FEI FALCON II (4k x 4k)

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Image acquisition

Image acquisitionNumber of digital images: 3850

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 110000
3D reconstructionAlgorithm: FOURIER SPACE / Software: RELION / Resolution: 3.8 A / Resolution method: FSC 0.143 CUT-OFF

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Atomic model buiding

Output model

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