48-nm doublet microtubule from Tetrahymena thermophila strain MEC17
Map data
Composite map of the 48-nm repeating unit of the doublet microtubule from Tetrahymena thermophila mutant strain MEC17
Sample
Organelle or cellular component: 48-nm repeat unit of microtubule doublet from Tetrahymena thermophila strain MEC17
Protein or peptide: x 45 types
Ligand: x 3 types
Keywords
Cilia / Axoneme / Doublet Microtubule / Microtubule Inner Protein / STRUCTURAL PROTEIN
Function / homology
Function and homology information
membrane-bounded organelle / axoneme assembly / axonemal microtubule / intracellular organelle / nucleoside-diphosphate kinase / CTP biosynthetic process / UTP biosynthetic process / motile cilium / GTP biosynthetic process / nucleoside diphosphate kinase activity ...membrane-bounded organelle / axoneme assembly / axonemal microtubule / intracellular organelle / nucleoside-diphosphate kinase / CTP biosynthetic process / UTP biosynthetic process / motile cilium / GTP biosynthetic process / nucleoside diphosphate kinase activity / axoneme / meiotic cell cycle / cell projection / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / cilium / structural constituent of cytoskeleton / microtubule cytoskeleton organization / mitotic cell cycle / microtubule / cytoskeleton / hydrolase activity / phosphorylation / GTPase activity / calcium ion binding / GTP binding / ATP binding / metal ion binding / nucleus / cytoplasm Similarity search - Function
CCDC81, HU domain 1 / CCDC81, HU domain 2 / CCDC81 eukaryotic HU domain 1 / CCDC81 eukaryotic HU domain 2 / Sperm-tail PG-rich repeat / Sperm-tail PG-rich repeat / FAM183 family / FAM183A and FAM183B related / Cilia- and flagella-associated protein 77 / Cilia- and flagella-associated protein 77 ...CCDC81, HU domain 1 / CCDC81, HU domain 2 / CCDC81 eukaryotic HU domain 1 / CCDC81 eukaryotic HU domain 2 / Sperm-tail PG-rich repeat / Sperm-tail PG-rich repeat / FAM183 family / FAM183A and FAM183B related / Cilia- and flagella-associated protein 77 / Cilia- and flagella-associated protein 77 / Uncharacterised protein FAM166/UPF0605 / Ciliary microtubule inner protein 2B-like / Nucleoside diphosphate kinase 7 / Meiosis-specific nuclear structural protein 1 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / Calmodulin-binding / Enkurin domain profile. / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Dienelactone hydrolase / Dienelactone hydrolase family / Nucleoside diphosphate kinase, active site / Nucleoside diphosphate kinase (NDPK) active site signature. / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / EF-hand domain pair / Mir domain superfamily / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Alpha/Beta hydrolase fold / Armadillo-type fold / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily Similarity search - Domain/homology
Flagellar FliJ protein / Uncharacterized protein / RIB43A protein / EF hand protein / Uncharacterized protein / Meiosis-specific nuclear structural protein 1 / EF-hand domain-containing family member C2 / DNA polymerase delta C4-type zinc-finger protein / Sperm-tail PG-rich repeat protein / Parkin co-regulated protein ...Flagellar FliJ protein / Uncharacterized protein / RIB43A protein / EF hand protein / Uncharacterized protein / Meiosis-specific nuclear structural protein 1 / EF-hand domain-containing family member C2 / DNA polymerase delta C4-type zinc-finger protein / Sperm-tail PG-rich repeat protein / Parkin co-regulated protein / Ptype ATPase, putative / Uncharacterized protein / EF-hand protein / EF-hand pair protein / EF-hand pair protein / Uncharacterized protein / Uncharacterized protein / Parkin co-regulated protein / Uncharacterized protein / Tubulin alpha chain / Tubulin beta chain / Uncharacterized protein / Uncharacterized protein / Cilia- and flagella-associated protein 20 / EF hand protein, putative / Sperm-tail PG-rich repeat protein / Cilia- and flagella-associated protein 52 / Uncharacterized protein / Uncharacterized protein / Dienelactone hydrolase family protein / Ciliary microtubule inner protein 2A-C-like domain-containing protein / Uncharacterized protein / Uncharacterized protein / Ciliary microtubule inner protein 2A-C-like domain-containing protein / Trichohyalin-plectin-homology domain-containing protein / STOP protein / EF hand protein / Cilia- and flagella-associated protein 53 / Protofilament ribbon protein / Uncharacterized protein / Sperm-tail PG-rich repeat protein / Cilia- and flagella-associated protein 45 / Nucleoside diphosphate kinase Similarity search - Component
Biological species
Tetrahymena thermophila (eukaryote)
Method
single particle reconstruction / cryo EM / Resolution: 4.1 Å
Journal: Elife / Year: 2024 Title: Effect of α-tubulin acetylation on the doublet microtubule structure. Authors: Shun Kai Yang / Shintaroh Kubo / Corbin Steven Black / Katya Peri / Daniel Dai / Thibault Legal / Melissa Valente-Paterno / Jacek Gaertig / Khanh Huy Bui / Abstract: Acetylation of α-tubulin at the lysine 40 residue (αK40) by αTAT1/MEC-17 acetyltransferase modulates microtubule properties and occurs in most eukaryotic cells. Previous literatures suggest that ...Acetylation of α-tubulin at the lysine 40 residue (αK40) by αTAT1/MEC-17 acetyltransferase modulates microtubule properties and occurs in most eukaryotic cells. Previous literatures suggest that acetylated microtubules are more stable and damage resistant. αK40 acetylation is the only known microtubule luminal post-translational modification site. The luminal location suggests that the modification tunes the lateral interaction of protofilaments inside the microtubule. In this study, we examined the effect of tubulin acetylation on the doublet microtubule (DMT) in the cilia of using a combination of cryo-electron microscopy, molecular dynamics, and mass spectrometry. We found that αK40 acetylation exerts a small-scale effect on the DMT structure and stability by influencing the lateral rotational angle. In addition, comparative mass spectrometry revealed a link between αK40 acetylation and phosphorylation in cilia.
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Controller
Yorodumi
Open data
Basic information
Map data
Sample
Keywords
Cilia / 
Function and homology information
Authors
Canada, 1 items 
Citation
Structure visualization
Downloads & links
emd_40436.png
http://ftp.pdbj.org/pub/emdb/structures/EMD-40436
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Sample components
Processing
Electron microscopy
