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Yorodumi- EMDB-40236: Structure of C-terminal domains of Walker B mutated MCM8/9 hetero... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-40236 | |||||||||
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Title | Structure of C-terminal domains of Walker B mutated MCM8/9 heterohexamer complex with ADP | |||||||||
Map data | ||||||||||
Sample |
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Keywords | DNA Replication / DNA repair / DNA BINDING PROTEIN | |||||||||
Function / homology | Function and homology information MutLbeta complex binding / MutSbeta complex binding / recombinational interstrand cross-link repair / MCM8-MCM9 complex / male gamete generation / mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication / MutSalpha complex binding / CDC6 association with the ORC:origin complex / female gamete generation / E2F-enabled inhibition of pre-replication complex formation ...MutLbeta complex binding / MutSbeta complex binding / recombinational interstrand cross-link repair / MCM8-MCM9 complex / male gamete generation / mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication / MutSalpha complex binding / CDC6 association with the ORC:origin complex / female gamete generation / E2F-enabled inhibition of pre-replication complex formation / Unwinding of DNA / MCM complex / DNA duplex unwinding / Activation of the pre-replicative complex / Activation of ATR in response to replication stress / protein localization to chromatin / helicase activity / double-strand break repair via homologous recombination / Orc1 removal from chromatin / large ribosomal subunit / single-stranded DNA binding / chromosome / DNA helicase / protein stabilization / structural constituent of ribosome / translation / DNA damage response / chromatin binding / enzyme binding / ATP hydrolysis activity / nucleoplasm / ATP binding / nucleus Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.94 Å | |||||||||
Authors | Li C / Gao Y | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Nucleic Acids Res / Year: 2023 Title: Activity, substrate preference and structure of the HsMCM8/9 helicase. Authors: David R McKinzey / Chuxuan Li / Yang Gao / Michael A Trakselis / Abstract: The minichromosomal maintenance proteins, MCM8 and MCM9, are more recent evolutionary additions to the MCM family, only cooccurring in selected higher eukaryotes. Mutations in these genes are ...The minichromosomal maintenance proteins, MCM8 and MCM9, are more recent evolutionary additions to the MCM family, only cooccurring in selected higher eukaryotes. Mutations in these genes are directly linked to ovarian insufficiency, infertility, and several cancers. MCM8/9 appears to have ancillary roles in fork progression and recombination of broken replication forks. However, the biochemical activity, specificities and structures have not been adequately illustrated, making mechanistic determination difficult. Here, we show that human MCM8/9 (HsMCM8/9) is an ATP dependent DNA helicase that unwinds fork DNA substrates with a 3'-5' polarity. High affinity ssDNA binding occurs in the presence of nucleoside triphosphates, while ATP hydrolysis weakens the interaction with DNA. The cryo-EM structure of the HsMCM8/9 heterohexamer was solved at 4.3 Å revealing a trimer of heterodimer configuration with two types of interfacial AAA+ nucleotide binding sites that become more organized upon binding ADP. Local refinements of the N or C-terminal domains (NTD or CTD) improved the resolution to 3.9 or 4.1 Å, respectively, and shows a large displacement in the CTD. Changes in AAA+ CTD upon nucleotide binding and a large swing between the NTD and CTD likely implies that MCM8/9 utilizes a sequential subunit translocation mechanism for DNA unwinding. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_40236.map.gz | 118 MB | EMDB map data format | |
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Header (meta data) | emd-40236-v30.xml emd-40236.xml | 17.4 KB 17.4 KB | Display Display | EMDB header |
Images | emd_40236.png | 173.6 KB | ||
Others | emd_40236_half_map_1.map.gz emd_40236_half_map_2.map.gz | 116.1 MB 116.1 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-40236 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-40236 | HTTPS FTP |
-Validation report
Summary document | emd_40236_validation.pdf.gz | 962.4 KB | Display | EMDB validaton report |
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Full document | emd_40236_full_validation.pdf.gz | 962 KB | Display | |
Data in XML | emd_40236_validation.xml.gz | 14 KB | Display | |
Data in CIF | emd_40236_validation.cif.gz | 16.5 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-40236 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-40236 | HTTPS FTP |
-Related structure data
Related structure data | 8s94MC 8s91C 8s92C C: citing same article (ref.) M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_40236.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.11 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_40236_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_40236_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Structure of Walker B mutated MCM8/9 heterohexamer complex with ADP
Entire | Name: Structure of Walker B mutated MCM8/9 heterohexamer complex with ADP |
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Components |
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-Supramolecule #1: Structure of Walker B mutated MCM8/9 heterohexamer complex with ADP
Supramolecule | Name: Structure of Walker B mutated MCM8/9 heterohexamer complex with ADP type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 500 KDa |
-Macromolecule #1: DNA helicase MCM8
Macromolecule | Name: DNA helicase MCM8 / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO / EC number: DNA helicase |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 93.817703 KDa |
Recombinant expression | Organism: Trichoplusia ni (cabbage looper) |
Sequence | String: MNGEYRGRGF GRGRFQSWKR GRGGGNFSGK WREREHRPDL SKTTGKRTSE QTPQFLLSTK TPQSMQSTLD RFIPYKGWKL YFSEVYSDS SPLIEKIQAF EKFFTRHIDL YDKDEIERKG SILVDFKELT EGGEVTNLIP DIATELRDAP EKTLACMGLA I HQVLTKDL ...String: MNGEYRGRGF GRGRFQSWKR GRGGGNFSGK WREREHRPDL SKTTGKRTSE QTPQFLLSTK TPQSMQSTLD RFIPYKGWKL YFSEVYSDS SPLIEKIQAF EKFFTRHIDL YDKDEIERKG SILVDFKELT EGGEVTNLIP DIATELRDAP EKTLACMGLA I HQVLTKDL ERHAAELQAQ EGLSNDGETM VNVPHIHARV YNYEPLTQLK NVRANYYGKY IALRGTVVRV SNIKPLCTKM AF LCAACGE IQSFPLPDGK YSLPTKCPVP VCRGRSFTAL RSSPLTVTMD WQSIKIQELM SDDQREAGRI PRTIECELVH DLV DSCVPG DTVTITGIVK VSNAEEGSRN KNDKCMFLLY IEANSISNSK GQKTKSSEDG CKHGMLMEFS LKDLYAIQEI QAEE NLFKL IVNSLCPVIF GHELVKAGLA LALFGGSQKY ADDKNRIPIR GDPHILVVGD PGLGKSQMLQ AACNVAPRGV YVCGN TTTT SGLTVTLSKD SSSGDFALEA GALVLGDQGI CGIDQFDKMG NQHQALLEAM EQQSISLAKA GVVCSLPART SIIAAA NPV GGHYNKAKTV SENLKMGSAL LSRFDLVFIL LDTPNEHHDH LLSEHVIAIR AGKQRTISSA TVARMNSQDS NTSVLEV VS EKPLSERLKV VPGETIDPIP HQLLRKYIGY ARQYVYPRLS TEAARVLQDF YLELRKQSQR LNSSPITTRQ LESLIRLT E ARARLELREE ATKEDAEDIV EIMKYSMLGT YSDEFGNLDF ERSQHGSGMS NRSTAKRFIS ALNNVAERTY NNIFQFHQL RQIAKELNIQ VADFENFIGS LNDQGYLLKK GPKVYQLQTM UniProtKB: DNA helicase MCM8 |
-Macromolecule #2: DNA helicase MCM9
Macromolecule | Name: DNA helicase MCM9 / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO / EC number: DNA helicase |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 127.485594 KDa |
Recombinant expression | Organism: Trichoplusia ni (cabbage looper) |
Sequence | String: MNSDQVTLVG QVFESYVSEY HKNDILLILK ERDEDAHYPV VVNAMTLFET NMEIGEYFNM FPSEVLTIFD SALRRSALTI LQSLSQPEA VSMKQNLHAR ISGLPVCPEL VREHIPKTKD VGHFLSVTGT VIRTSLVKVL EFERDYMCNK CKHVFVIKAD F EQYYTFCR ...String: MNSDQVTLVG QVFESYVSEY HKNDILLILK ERDEDAHYPV VVNAMTLFET NMEIGEYFNM FPSEVLTIFD SALRRSALTI LQSLSQPEA VSMKQNLHAR ISGLPVCPEL VREHIPKTKD VGHFLSVTGT VIRTSLVKVL EFERDYMCNK CKHVFVIKAD F EQYYTFCR PSSCPSLESC DSSKFTCLSG LSSSPTRCRD YQEIKIQEQV QRLSVGSIPR SMKVILEDDL VDSCKSGDDL TI YGIVMQR WKPFQQDVRC EVEIVLKANY IQVNNEQSSG IIMDEEVQKE FEDFWEYYKS DPFAGRNVIL ASLCPQVFGM YLV KLAVAM VLAGGIQRTD ATGTRVRGES HLLLVGDPGT GKSQFLKYAA KITPRSVLTT GIGSTSAGLT VTAVKDSGEW NLEA GALVL ADAGLCCIDQ FNSLKEHDRT SIHEAMEQQT ISVAKAGLVC KLNTRTTILA ATNPKGQYDP QESVSVNIAL GSPLL SRFD LILVLLDTKN EDWDRIISSF ILENKGYPSK SEKLWSMEKM KTYFCLIRNL QPTLSDVGNQ VLLRYYQMQR QSDCRN AAR TTIRLLESLI RLAEAHARLM FRDTVTLEDA ITVVSVMESS MQGGALLGGV NALHTSFPEN PGEQYQRQCE LILEKLE LQ SLLSEELRRL ERLQNQSVHQ SQPRVLEVET TPGSLRNGPG EESNFRTSSQ QEINYSTHIF SPGGSPEGSP VLDPPPHL E PNRSTSRKHS AQHKNNRDDS LDWFDFMATH QSEPKNTVVV SPHPKTSGEN MASKISNSTS QGKEKSEPGQ RSKVDIGLL PSPGETGVPW RADNVESNKK KRLALDSEAA VSADKPDSVL THHVPRNLQK LCKERAQKLC RNSTRVPAQC TVPSHPQSTP VHSPDRMLD SPKRKRPKSL AQVEEPAIEN VKPPGSPVAK LAKFTFKQKS KLIHSFEDHS HVSPGATKIA VHSPKISQRR T RRDAALPV KRPGKLTSTP GNQISSQPQG ETKEVSQQPP EKHGPREKVM CAPEKRIIQP ELELGNETGC AHLTCEGDKK EE VSGSNKS GKVHACTLAR LANFCFTPPS ESKSKSPPPE RKNRGERGPS SPPTTTAPMR VSKRKSFQLR GSTEKLIVSK ESL FTLPEL GDEAFDCDWD EEMRKKS UniProtKB: Large ribosomal subunit protein uL22 |
-Macromolecule #3: ADENOSINE-5'-DIPHOSPHATE
Macromolecule | Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 3 / Number of copies: 6 / Formula: ADP |
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Molecular weight | Theoretical: 427.201 Da |
Chemical component information | ChemComp-ADP: |
-Macromolecule #4: MAGNESIUM ION
Macromolecule | Name: MAGNESIUM ION / type: ligand / ID: 4 / Number of copies: 6 / Formula: MG |
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Molecular weight | Theoretical: 24.305 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 0.5 mg/mL |
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Buffer | pH: 8 |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK I |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 49.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.7000000000000001 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.94 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 227818 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |