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- EMDB-40194: The Type 9 Secretion System Extended Translocon - SprA-PorV-PPI-R... -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-40194
TitleThe Type 9 Secretion System Extended Translocon - SprA-PorV-PPI-RemZ-SkpA-SprE complex
Map dataComposite volume used for refinement of coordinates created from EMD-29911 and EMD-40085 the associated consensus volume is EMD-40086
Sample
  • Complex: Type 9 Secretion System Extended Translocon
    • Protein or peptide: Protein involved in gliding motility SprA
    • Protein or peptide: Peptidyl-prolyl cis-trans isomeraseProlyl isomerase
    • Protein or peptide: Type IX secretion system protein PorV domain-containing protein
    • Protein or peptide: RemZ
    • Protein or peptide: Periplasmic chaperone for outer membrane proteins Skp
    • Protein or peptide: SprE
  • Ligand: Lauryl Maltose Neopentyl Glycol
  • Ligand: alpha-D-mannopyranose
  • Ligand: water
KeywordsT9SS / Type IX Secretion System Translocon / Protein Secretion PorV - SprA - PPI - RemZ Complex / MEMBRANE PROTEIN
Function / homology
Function and homology information


peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / unfolded protein binding
Similarity search - Function
Chaperone protein Skp / Skp domain superfamily / Outer membrane protein (OmpH-like) / Outer membrane protein (OmpH-like) / Type IX secretion system protein PorV / : / Type IX secretion system protein PorV / Gliding motility protein SprA N-terminal domain / Cell surface SprA / Motility related/secretion protein ...Chaperone protein Skp / Skp domain superfamily / Outer membrane protein (OmpH-like) / Outer membrane protein (OmpH-like) / Type IX secretion system protein PorV / : / Type IX secretion system protein PorV / Gliding motility protein SprA N-terminal domain / Cell surface SprA / Motility related/secretion protein / Tetratricopeptide repeat / FKBP-type peptidyl-prolyl cis-trans isomerase domain profile. / FKBP-type peptidyl-prolyl cis-trans isomerase domain / FKBP-type peptidyl-prolyl cis-trans isomerase / Peptidyl-prolyl cis-trans isomerase domain superfamily / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat / Prokaryotic membrane lipoprotein lipid attachment site profile. / Tetratricopeptide-like helical domain superfamily
Similarity search - Domain/homology
Periplasmic chaperone for outer membrane proteins Skp / Protein involved in gliding motility SprA / SprE / Peptidyl-prolyl cis-trans isomerase / Type IX secretion system protein PorV domain-containing protein / T9SS sorting signal type C domain-containing protein
Similarity search - Component
Biological speciesFlavobacterium johnsoniae UW101 (bacteria) / Flavobacterium johnsoniae (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.2 Å
AuthorsDeme JC / Lea SM
Funding support United Kingdom, European Union, United States, 6 items
OrganizationGrant numberCountry
Wellcome Trust219477/Z/19/Z United Kingdom
Wellcome Trust107929/Z/15/Z United Kingdom
European Research Council (ERC)833713European Union
Biotechnology and Biological Sciences Research Council (BBSRC)BB/S007474/1 United Kingdom
Medical Research Council (MRC, United Kingdom)MR/S021264/1 United Kingdom
National Institutes of Health/National Cancer Institute (NIH/NCI)CCR Core Funding for Lea Group United States
CitationJournal: Nat Microbiol / Year: 2024
Title: The Type 9 Secretion System caught in the act of transport
Authors: Lauber F / Deme JC / Liu X / Kjaer A / Miller HL / Alcock F / Lea SM / Berks BC
History
DepositionMar 21, 2023-
Header (metadata) releaseJan 31, 2024-
Map releaseJan 31, 2024-
UpdateJan 31, 2024-
Current statusJan 31, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_40194.png

Downloads & links

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Map

FileDownload / File: emd_40194.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComposite volume used for refinement of coordinates created from EMD-29911 and EMD-40085 the associated consensus volume is EMD-40086
Voxel sizeX=Y=Z: 0.832 Å
Density
Contour LevelBy AUTHOR: 8.539999999999999
Minimum - Maximum-40.660240000000002 - 80.750113999999996
Average (Standard dev.)0.014444056 (±1.270833)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions448448448
Spacing448448448
CellA=B=C: 372.73602 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Type 9 Secretion System Extended Translocon

EntireName: Type 9 Secretion System Extended Translocon
Components
  • Complex: Type 9 Secretion System Extended Translocon
    • Protein or peptide: Protein involved in gliding motility SprA
    • Protein or peptide: Peptidyl-prolyl cis-trans isomeraseProlyl isomerase
    • Protein or peptide: Type IX secretion system protein PorV domain-containing protein
    • Protein or peptide: RemZ
    • Protein or peptide: Periplasmic chaperone for outer membrane proteins Skp
    • Protein or peptide: SprE
  • Ligand: Lauryl Maltose Neopentyl Glycol
  • Ligand: alpha-D-mannopyranose
  • Ligand: water

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Supramolecule #1: Type 9 Secretion System Extended Translocon

SupramoleculeName: Type 9 Secretion System Extended Translocon / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#6 / Details: SkpA-SprE-Nterm SprA part of complex
Source (natural)Organism: Flavobacterium johnsoniae UW101 (bacteria)

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Macromolecule #1: Protein involved in gliding motility SprA

MacromoleculeName: Protein involved in gliding motility SprA / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 270.221688 KDa
SequenceString: MRKICIFLLV LFCGNVLRSQ VKPAVQDTTK TQFSVGKMEL ENPPSILSAY KYDPITDRYI YTTSVDGFSI DYPLVLTPKE YEDLLLKES RRDYFRKKMD AIDGKKTGAE AAKKDLLPRY YINSSLFESI FGSNTIDVKP TGSVEMDLGV RYTKQDNPAF S PRNRSSLT ...String:
MRKICIFLLV LFCGNVLRSQ VKPAVQDTTK TQFSVGKMEL ENPPSILSAY KYDPITDRYI YTTSVDGFSI DYPLVLTPKE YEDLLLKES RRDYFRKKMD AIDGKKTGAE AAKKDLLPRY YINSSLFESI FGSNTIDVKP TGSVEMDLGV RYTKQDNPAF S PRNRSSLT FDFDQRISMS LMGKIGTRLE VNANYDTQST FAFQNLFKLA YTPSEDDIIQ KVEVGNVSMP LNSTLIRGAQ SL FGVKTQL QFGRTTITGV FSEQKSQTKS VVAENGGTVQ NFDLYALDYD NDRHFFLSQY FRNKYDVSLK NYPFIDSRVQ ITR LEVWVT NKQNRVTTTG GGNNLRNIIA LQDLGEAQVS GVPDNEVVVI SSTAGFFNNP IDSPTSNTNN KYDPATIGQA GSFL NSNIR EIVTAKSGFN NTNVSEATDY SVLENARKLT TNEYTFNPQL GYISLQQRLA NDEILAVAFE YTVGGKVYQV GEFGS DGVD ATVVTGNNSS NQAIITQSLV LKMLKSNLTN VKNPVWNLMM KNVYQIPQAY QIKQDDFRLN ILYTDPSPIN YITPVQ GSS FPPNPAPDSK VEQTPLLNVF NLDRLNYNND PQAGGDGFFD YIPGVTVDVQ NGRVIFTTKE PFGELIFNKL QTGAGES YN DPTTYNANQQ KYVFRNMYRN TQAGALQDSD KNKFLLRGKY KSSGSNGIPI GAFNVPQGSV VVTAAGRVLV EGIDYSVD Y QLGRVQILDP SLQASNTPIE VSLENNSIFG QQTRRFMGFN IEHKISDKFV IGGTYLKMTE RPFTQKSTYG QESVNNTIF GFNGNYSTEV PFLTRLANKL PNIDTDVPSN LSIRGEVAFL RPDAPKASDF QGEATIYVDD FEGSQSTIDM RSAYAWSLAS TPFITSIND NTFNANSNTL EYGFKRAKLS WYTIDPVFYS SKPSGISNDD LSLNTTRRIY SRELYPNTDI AQGQIQVVNT L DLTYYPGE RGPYNNNPSF GASNPSANFG GIMRALNSTN FEQGNVEYIQ FWVLDPYVGN GESPATNAGK IYFNLGEISE DV LKDGRKQ YENGLGPDQV MVNPQPLWGD VPASQSLIYA FDTNPDNRKN QDVGLDGLPS SREGSIYTNY AGEADPAGDD YTY YLNADG GVLERYKNYN GTEGNSAVSI NDPNRGSTTL PDVEDINRDN TMSTINAYYE YSIDVKPGMQ VGENYITDIR EVTN VDLPN GGTTNARWIQ FKIPVSQPQN TIGNITDFRS IRFMRMFMTG FNSQMTVRFG ALDLVRGEWR RYTGTLDAND QNPDD DGVE FDVAAVNIQE NGTKCPVNYV MPPGVQREQL YNNNTVINQN EQALAVRIGG AGLQYQDSRA VFKNVSVDMR QYKKLK MFL HAESLPNQPT LEDDEMVGFI RFGNDFTQNF YQVEIPLKVT KTGGSCSISP DLVWMDDNSI DLALDLLTRM KIKAMSI DI NSSKRDVNGI YYPDNDPDLE GGDGDGKLTL GIKGNPNFGL VRNLMVGVKS RADHKDIKGE VWFNELRLAD LENKGGMA A ILNVDTNMAD FATVSATGRK STIGFGSLEQ GANERDREDV QQYNIVTNLN LGKLLPKKWG INLPFNYAIG EEVITPEYD PFNQDIKLDQ LIRETTDQAE KDNIRTRAID YTKRKSINFI GVRKDRAPEQ KPHVYDIENF TFSQSYNQVE RHDYEVADYE DEQSNSAVN YAYTFQPKEV VPFKSTKFMK KSEYWKLLSD FNFNYLPSNI SFNTNILRQS NRQQFREVEV EGIGLDPLYR R NFAFNYQY GFGFNLTKSL KLNYSATSNN IVRNFLNDDN SPKEDFNIWD DYLDIGTPNQ HAQQLVLNYD IPINKIPIFG FV KASYSYT ADYMWQRSST AFSEYEDPNG TVYDLGNTIQ NSNSNTLTTT LNMNTLYKYL GLTPGAKKTA KPKTAAPPKP GEK IVNTAK PVVSSSPFYD GLIGVLTSIK NVQINYTKNS GTVLPGYTPS VGFLGTSKPS LGFVFGSQDD VRYEAAKRGW LTTY QDFNQ SFTQVSNKLL KVTANIDLLP DLKVDLSMDR SYSENTSEQY SVDPSTNEYK PLSPYTYGMF SISTVMIKTA FSPSD ETQS AAFDDFRSNR LIIANRLAEG HYGSGVAIPR YGDANNPIPA ETDPNYAVYT ANQGYPIGYT KSNQAVLLPA FLAAYT GSD ASSSSTNIFR SFPIPNWSIK YNGLMRYKYF KDKFKRFSLQ HNYRASYTIN QFRSNFDYNS SPKVQDVNTN FYNEIIM SN VNLVEQFSPL IRMDFELKSS LRVLSEIKKD RALSMSFDNN LLTEVKGMEY IIGLGYRFKD VIFSSRLADN PTGIIKSD I NIKADFSLRN NETLVRYLDY DNNQLAAGQN IWSLKLTADY SFSKNLTAIF YYDHSFSKAV ISTSFPLTNI RSGFTLRYN FGN

UniProtKB: Protein involved in gliding motility SprA

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Macromolecule #2: Peptidyl-prolyl cis-trans isomerase

MacromoleculeName: Peptidyl-prolyl cis-trans isomerase / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 19.219141 KDa
SequenceString:
MKQLLTALLS LTLFISCSKD KDEVKDYTAE NEKEIVDYLA QNNLTAQRTN SGLYYIITKE GSSESEGENP GEEENTGEGE NTEENENDG HPTLNSNITV IYKGYFTNGK VFDESTEGVS YSLRTLIPGW KEGIPLLKSG GEIQLFVPAH LGYGSNGNKT V PGGAVLIF EITLVSVN

UniProtKB: Peptidyl-prolyl cis-trans isomerase

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Macromolecule #3: Type IX secretion system protein PorV domain-containing protein

MacromoleculeName: Type IX secretion system protein PorV domain-containing protein
type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 44.210043 KDa
SequenceString: MKKISLLLIC LLITTFAKAQ DIERPITTGV PFLLVAADAR AAGLGDQGVA TSSDVFSQQW NPAKYAFAED AQGLSISYTP YLTDLANDI SLGQVTYYNK INDRSAFAGS FRYFGFGGIE LRQTGDPNEP TREVNPNEFA LDGSYSLKLS ETFSMAVAAR Y IRSNLKVA ...String:
MKKISLLLIC LLITTFAKAQ DIERPITTGV PFLLVAADAR AAGLGDQGVA TSSDVFSQQW NPAKYAFAED AQGLSISYTP YLTDLANDI SLGQVTYYNK INDRSAFAGS FRYFGFGGIE LRQTGDPNEP TREVNPNEFA LDGSYSLKLS ETFSMAVAAR Y IRSNLKVA TEEIDASAAG SFAVDVAGFY QSEEIAYSDF NGRWRAGFNI QNLGPKISYD HDDLSANFLP ANLRVGGGFD FI FDDYNKL GVSLELTKLL VPTPPGPGTP YDANGDGDFT DPGDISQSQA DEANYKKYKD IGWVSGIFKS FGDAPGGFSE ELK EITYSA AAEYMYQDAF AMRLGYYHES PMKGAKQFFS LGAGFKYSMI KVDVSYLFSA SKVKNPLENT LRFSLTFNFG DKYE TY

UniProtKB: Type IX secretion system protein PorV domain-containing protein

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Macromolecule #4: RemZ

MacromoleculeName: RemZ / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 113.146023 KDa
SequenceString: MDVQAWGGGG AGGGASGAVL DGRAAAGGGG GAYARSNITV AAGATLNASV AGTTTNALVS GAAVNGAAGG SSTILGFETS ILALGGGGG GANNAGGTPA GGAGGSAASS VGNVSKLDGA AGGNGVTGAI GLLTVSGAGG TAGGGGGAGG AGVASVALGN G PGNAGTAP ...String:
MDVQAWGGGG AGGGASGAVL DGRAAAGGGG GAYARSNITV AAGATLNASV AGTTTNALVS GAAVNGAAGG SSTILGFETS ILALGGGGG GANNAGGTPA GGAGGSAASS VGNVSKLDGA AGGNGVTGAI GLLTVSGAGG TAGGGGGAGG AGVASVALGN G PGNAGTAP GGGGSGAMQS LLGGAQIGGS GAAGRVIITY TCPTYSITGI SAANVCNSVG TTSVVTLTSS GGGLPIGPYV VT YNRSNPS GTGLTAIMNV TTPGTGTFTA AGLNVIGTSN ITVTNLTSAA CSSNISTNNV ASLTVFAATV GGTLAGTATV CSG ATSGTL TLSGQTGSII KWESSVSPFT VWTTIPNTTN TYTSGALTET SQFRAVIQNG NCAVVNSSIA TITVNPLPQG SLSA NGPFC VTGSGQLTFT ATAGTGPYTI VYKENGGADR TAANISSGVA FPTFTTPVTT TTVYTLVSVT GANTCSRSSG FTNNT ATIT VNSRIATPGF GTVTQPDCVT STGSVVLTGL PAGSWTITQS GTASQTYNSS GTTYTISNLA VGNYTFTVQD AANCPS LAT STLTLIAPVV NIWNGTSWSK GSPPISTDVV RFSGNYSTTG NLSGCSLIVD SGFTVTVNSN HTLTISNAVT NNGGQLI FE NNSSLLQTNN VTNVGNITYK RITPPVRRYD LTYWSSPITR TPPFTLYDLS PGTLADKYYS YDPVAGWVIS FNGTQQMV P GRGYVVRAPQ TNDLNTGANY LGAFVGVPNN GPISVSLGTA EAFQLLGNPY PSAIYADQFI ANNSANLYGT LYFWTHNSL PSSSTPGGAQ YNYDNNDYAV YNLSGSIIVG GMTGQGATTP GNQSAPLGYI AAGQGFFVVS KTAGNAVFTN SMRVAANNTQ FYKTNKSAI ERHRVWINLT NTQGAFKQLL IGYIEGATNF WDHNYDAITA DANPHLDFYS INEGQNLVIQ GRSLPFNESD V VPLGYRSA IAGEFSISLD HADGDLTNHA VYLEDKLTNT LHNLQTSNYT FNTAIGTFSD RFVIRYTTAT LGTDDFENQT NS FYVSVKD KTIKLNSTED VMREVSIFDI SGKLLYNNKK VENTEFQVSN FQSGNQVLIV KVTLDNGNII TKKIVFN

UniProtKB: T9SS sorting signal type C domain-containing protein

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Macromolecule #5: Periplasmic chaperone for outer membrane proteins Skp

MacromoleculeName: Periplasmic chaperone for outer membrane proteins Skp / type: protein_or_peptide / ID: 5 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 39.64373 KDa
SequenceString: MRKQFLFIFL ALIVANTSQA QGKTTRIGYI DMEYILENVS DYKEAKSQLE LKAQKWKQEI EAKKLNINSL KEGLKTEKAL LTKELIEER ETEIKFQENE MLDYQQKQFG ADGNLMRQKA ALAKPIQDQV FTAVQDIAEA KNYDFIFDKS SDLTMLFSNK R FDISDQVI ...String:
MRKQFLFIFL ALIVANTSQA QGKTTRIGYI DMEYILENVS DYKEAKSQLE LKAQKWKQEI EAKKLNINSL KEGLKTEKAL LTKELIEER ETEIKFQENE MLDYQQKQFG ADGNLMRQKA ALAKPIQDQV FTAVQDIAEA KNYDFIFDKS SDLTMLFSNK R FDISDQVI RILNRTDKRE QLNKKQLKEQ EAKENRENEI DENPAMADRQ KALDERRAAR EKLIEDRRLE QEAKKKEYDD RR KAMQAER DAKKNGTVSE TAKTTEAAKT TEAVKTDATA KPASTTETTT TPAETAASKA EERQKLYEQR KKELEERRKK ILE EREAAK KAKEAETQKT NTTNN

UniProtKB: Periplasmic chaperone for outer membrane proteins Skp

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Macromolecule #6: SprE

MacromoleculeName: SprE / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 101.274883 KDa
SequenceString: MKKNTLKYSF FLIFFLFLIA CSTKNNTFVN RNSHALSTKY NILYNGGLGL EKGLQAIKAN DQDNFWKMLP IEKMQFDENF SEGEKTKNP DFEKAETKAT KAIQKHSMNI GGRERNYQID EAYLMLGKAR YYDQRFIPAL EAFNYILYKY PNSSNIYTAK I WREKTNMR ...String:
MKKNTLKYSF FLIFFLFLIA CSTKNNTFVN RNSHALSTKY NILYNGGLGL EKGLQAIKAN DQDNFWKMLP IEKMQFDENF SEGEKTKNP DFEKAETKAT KAIQKHSMNI GGRERNYQID EAYLMLGKAR YYDQRFIPAL EAFNYILYKY PNSSNIYTAK I WREKTNMR LGNDAIVVKN INQLLKKTDL NKQTFSDANA LLAEAFLNLE ERDSAVAKLR IAEQFSRINE DRARYKFILG QM YQEVGNK DSATYYYDGV IHMNRKADRK YMMHAYAKKA QMYDYEKGND TIFLKTYNKL VADRENRPYY DVLFYEMGVF YDK KKDKEN ALKFYNKSLG RKSKDPYLMA SAYRNIGNMY FKNTDYTMAA KYYDSTLTKL NPKTREFAFI EKNRKNLDNV IKYE GIAKR NDSIIKVYGM PDSERKIYFE SYIAELKKKD EAKRILEEKE KEKLANVERN NSASSAPTAV NPNSLGKPAN MDTDG IRPP SGNDAVSTFY FYNPTTVAYG KLQFKKMWGN RTIGGNWRLS AIKAANDAAM LNDSINEAEA NKLKDTVVIE KYTTAF YEK QLPKTQIAID SIGKERNFAY YQLGLIYKEK FKEYTLASDK LEQLLRNNPE EKLILPSMYN LYKIYQITDP AKAEKIK SD ITNNYPGSRY AQILNNTNTD DIPSPEKEYQ KWYKLFQEEK FDVVLDNIDN LINQYSGDEI VSKFELLKAN TLGKVNGL E AYKKGLENVA DNYPNSDEGK NAREILEKQV PTLERLNFTT EDNKNWKILY LISNNDTKTL KQIEEAIRVF LLVENFERL TTSFDKYNRT QSFVAIHGLK SEAYAQDVAG VFRDDKKYKI AQPAIIISTE NYKVVQIKKN LEAYLTPKNP

UniProtKB: SprE

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Macromolecule #7: Lauryl Maltose Neopentyl Glycol

MacromoleculeName: Lauryl Maltose Neopentyl Glycol / type: ligand / ID: 7 / Number of copies: 1 / Formula: LMN
Molecular weightTheoretical: 1.005188 KDa
Chemical component information

ChemComp-AV0:
Lauryl Maltose Neopentyl Glycol

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Macromolecule #8: alpha-D-mannopyranose

MacromoleculeName: alpha-D-mannopyranose / type: ligand / ID: 8 / Number of copies: 6 / Formula: MAN
Molecular weightTheoretical: 180.156 Da
Chemical component information

ChemComp-MAN:
alpha-D-mannopyranose / Mannose

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Macromolecule #9: water

MacromoleculeName: water / type: ligand / ID: 9 / Number of copies: 1087 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.2 µm
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 61.2 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 546490

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