+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-38685 | |||||||||
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Title | Cryo-EM structure of tomato NRC2 filament | |||||||||
Map data | ||||||||||
Sample |
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Keywords | tomato / helper NLR / dimer / PLANT PROTEIN | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Solanum lycopersicum (tomato) | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 3.6 Å | |||||||||
Authors | Sun Y / Ma SC / Chai JJ | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: Cryo-EM structure of tomato NRC2 filament Authors: Ma SC / Chai JJ | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_38685.map.gz | 53.6 MB | EMDB map data format | |
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Header (meta data) | emd-38685-v30.xml emd-38685.xml | 14.2 KB 14.2 KB | Display Display | EMDB header |
Images | emd_38685.png | 79.6 KB | ||
Filedesc metadata | emd-38685.cif.gz | 6 KB | ||
Others | emd_38685_half_map_1.map.gz emd_38685_half_map_2.map.gz | 49.7 MB 49.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-38685 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-38685 | HTTPS FTP |
-Related structure data
Related structure data | 8xuvMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_38685.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.0979 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_38685_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_38685_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : NRC2 filament
Entire | Name: NRC2 filament |
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Components |
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-Supramolecule #1: NRC2 filament
Supramolecule | Name: NRC2 filament / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Solanum lycopersicum (tomato) |
-Macromolecule #1: NRC2
Macromolecule | Name: NRC2 / type: protein_or_peptide / ID: 1 Details: Sequence reference for NRC2 is not available in UniProt at the time of biocuration. Current sequence reference is from UniProt id A0A3Q7IF17. Regarding SlNRC2 with the accession number ...Details: Sequence reference for NRC2 is not available in UniProt at the time of biocuration. Current sequence reference is from UniProt id A0A3Q7IF17. Regarding SlNRC2 with the accession number Solyc10g047320 in the SGN database, here is the link: https://solgenomics.net/locus/36701/view. The source article for SlNRC2 is "Helper NLR proteins NRC2a/b and NRC3 but not NRC1 are required for Pto-mediated cell death and resistance in Nicotiana benthamiana" by Wu, Chih-Hang et al. The article was published in The New Phytologist, volume 209, issue 4, in 2016. The DOI is 10.1111/nph.13764. Number of copies: 12 / Enantiomer: LEVO |
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Source (natural) | Organism: Solanum lycopersicum (tomato) |
Molecular weight | Theoretical: 101.332078 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MANVAVEFLV ENLMQLLRDN VELISGVKEA AESLLQDLND FNAFLKQAAK CHINENEVLR ELVKKIRTVV NSAEDAIDKF VIEAKLHKD KGVTRVLDLP HYKRVKEVAG EIKAIRNKVR EIRQTDAIGL QALQDDDLSA RGSEERKPPV VEEDDVVGFD E EADIVINR ...String: MANVAVEFLV ENLMQLLRDN VELISGVKEA AESLLQDLND FNAFLKQAAK CHINENEVLR ELVKKIRTVV NSAEDAIDKF VIEAKLHKD KGVTRVLDLP HYKRVKEVAG EIKAIRNKVR EIRQTDAIGL QALQDDDLSA RGSEERKPPV VEEDDVVGFD E EADIVINR LLGESNHLEV VPVVGMPGLG KTTLANKIYK HPKIGYEFFT RIWVYVSQSY RRRELFLNII SKFTRNTKQY HG MCEEDLA DEIQEFLGKG GKYLVVLDDV WSDEAWERIK IAFPNNNKPN RVLLTTRDSK VAKQCNPIPH DLKFLTEDES WIL LEKKVF HKDKCPPELV LSGKSIAKKC KGLPLAIVVI AGALIGKGKT PREWKQVDDS VSEHLINRDH PENCNKLVQM SYDR LPYDL KACFLYCSAF PGGFQIPAWK LIRLWIAEGF IQYKGHLSLE CKGEDNLNDL INRNLVMVME RTSDGQIKTC RLHDM LHEF CRQEAMKEEN LFQEIKLGSE QYFPGKRELS TYRRLCIHSS VLDFFSTKPS AEHVRSFLSF SSKKIEMPSA DIPTIP KGF PLLRVLDVES INFSRFSREF YQLYHLRYVA FSSDSIKILP KLMGELWNIQ TIIINTQQRT LDIQANIWNM ERLRHLH TN SSAKLPVPVA PKNSKVTLVN QSLQTLSTIA PESCTEEVFA RTPNLKKLGI RGKISVLLDN KSAASLKNVK RLEYLENL K LINDSSIQTS KLRLPPAYIF PTKLRKLTLL DTWLEWKDMS ILGQLEHLEV LKMKENGFSG ESWESTGGFC SLLVLWIER TNLVSWKASA DDFPRLKHLV LICCDNLKEV PIALADIRSF QVMMLQNSTK TAAISARQIQ AKKDNQTQQG TKNIAFKLSI FPPDL UniProtKB: NRC1 |
-Macromolecule #2: INOSITOL HEXAKISPHOSPHATE
Macromolecule | Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 2 / Number of copies: 12 / Formula: IHP |
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Molecular weight | Theoretical: 660.035 Da |
Chemical component information | ChemComp-IHP: |
-Macromolecule #3: ADENOSINE-5'-DIPHOSPHATE
Macromolecule | Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 3 / Number of copies: 12 / Formula: ADP |
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Molecular weight | Theoretical: 427.201 Da |
Chemical component information | ChemComp-ADP: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | helical reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: NONE |
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Final angle assignment | Type: ANGULAR RECONSTITUTION |
Final reconstruction | Applied symmetry - Helical parameters - Δz: 64.04 Å Applied symmetry - Helical parameters - Δ&Phi: -55.85 ° Applied symmetry - Helical parameters - Axial symmetry: C3 (3 fold cyclic) Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 280426 |