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- EMDB-37465: Photosynthetic LH1-RC complex from the purple sulfur bacterium Al... -

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Basic information

Entry
Database: EMDB / ID: EMD-37465
TitlePhotosynthetic LH1-RC complex from the purple sulfur bacterium Allochromatium vinosum purified by sucrose density
Map dataPhotosynthetic LH1-RC complex from the purple sulfur bacterium Allochromatium vinosum
Sample
  • Complex: Photosynthetic LH1-RC complex from the purple sulfur phototrophic bacterium Allochromatium vinosum
    • Protein or peptide: x 9 types
  • Ligand: x 16 types
KeywordsPHOTOSYNTHESIS
Function / homology
Function and homology information


organelle inner membrane / plasma membrane-derived chromatophore membrane / plasma membrane light-harvesting complex / bacteriochlorophyll binding / photosynthetic electron transport in photosystem II / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / electron transfer activity / iron ion binding / heme binding ...organelle inner membrane / plasma membrane-derived chromatophore membrane / plasma membrane light-harvesting complex / bacteriochlorophyll binding / photosynthetic electron transport in photosystem II / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / electron transfer activity / iron ion binding / heme binding / membrane / metal ion binding / plasma membrane
Similarity search - Function
Photosynthetic reaction centre, cytochrome c subunit / Multihaem cytochrome, PRC subunit superfamily / Photosynthetic reaction centre cytochrome C subunit / Antenna complex, alpha subunit / Antenna complex, beta domain superfamily / Antenna complex, alpha subunit conserved site / Antenna complexes alpha subunits signature. / Light-harvesting protein B beta chain / Antenna complex, alpha/beta subunit / Light-harvesting complex ...Photosynthetic reaction centre, cytochrome c subunit / Multihaem cytochrome, PRC subunit superfamily / Photosynthetic reaction centre cytochrome C subunit / Antenna complex, alpha subunit / Antenna complex, beta domain superfamily / Antenna complex, alpha subunit conserved site / Antenna complexes alpha subunits signature. / Light-harvesting protein B beta chain / Antenna complex, alpha/beta subunit / Light-harvesting complex / Antenna complex alpha/beta subunit / Multiheme cytochrome c family profile. / Photosynthetic reaction centre, H subunit / Bacterial photosynthetic reaction centre, H-chain, C-terminal / Photosynthetic reaction centre, M subunit / Photosynthetic reaction centre, H subunit, N-terminal / Photosynthetic reaction centre, H subunit, N-terminal domain superfamily / Photosynthetic reaction centre, H-chain N-terminal region / PRC-barrel domain / PRC-barrel domain / Photosynthetic reaction centre, L subunit / PRC-barrel-like superfamily / Multiheme cytochrome superfamily / Photosynthetic reaction centre, L/M / Photosystem II protein D1/D2 superfamily / Photosynthetic reaction centre protein / Photosynthetic reaction center proteins signature. / Prokaryotic membrane lipoprotein lipid attachment site profile.
Similarity search - Domain/homology
Antenna complex alpha/beta subunit / Antenna complex alpha/beta subunit / Antenna complex alpha/beta subunit / Antenna complex alpha/beta subunit / Antenna complex alpha/beta subunit / Photosynthetic reaction center H subunit / Photosynthetic reaction center cytochrome c subunit / Reaction center protein L chain / Reaction center protein M chain
Similarity search - Component
Biological speciesAllochromatium vinosum DSM 180 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.24 Å
AuthorsTani K / Kanno R / Harada A / Kobayashi A / Minamino A / Nakamura N / Ji X-C / Purba ER / Hall M / Yu L-J ...Tani K / Kanno R / Harada A / Kobayashi A / Minamino A / Nakamura N / Ji X-C / Purba ER / Hall M / Yu L-J / Madigan MT / Mizoguchi A / Iwasaki K / Humbel BM / Kimura Y / Wang-Otomo Z-Y
Funding support Japan, 6 items
OrganizationGrant numberCountry
Japan Agency for Medical Research and Development (AMED)JP21am0101118 Japan
Japan Agency for Medical Research and Development (AMED)JP21am0101116 Japan
Japan Society for the Promotion of Science (JSPS)JP16H04174 Japan
Japan Society for the Promotion of Science (JSPS)JP18H05153 Japan
Japan Society for the Promotion of Science (JSPS)20H05086 Japan
Japan Society for the Promotion of Science (JSPS)20H02856 Japan
CitationJournal: Commun Biol / Year: 2024
Title: High-resolution structure and biochemical properties of the LH1-RC photocomplex from the model purple sulfur bacterium, Allochromatium vinosum
Authors: Tani K / Kanno R / Harada A / Kobayashi Y / Minamino A / Takenaka S / Nakamura N / Ji XC / Purba ER / Hall M / Yu LJ / Madigan MT / Mizoguchi A / Iwasaki K / Humbel BM / Kimura Y / Wang-Otomo ZY
History
DepositionSep 16, 2023-
Header (metadata) releaseFeb 21, 2024-
Map releaseFeb 21, 2024-
UpdateFeb 21, 2024-
Current statusFeb 21, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_37465.png

Downloads & links

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Map

FileDownload / File: emd_37465.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPhotosynthetic LH1-RC complex from the purple sulfur bacterium Allochromatium vinosum
Voxel sizeX=Y=Z: 0.606 Å
Density
Contour LevelBy AUTHOR: 0.013
Minimum - Maximum-0.056558464 - 0.14307253
Average (Standard dev.)0.0000512537 (±0.003502972)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 290.88 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Odd half map

Fileemd_37465_half_map_1.map
AnnotationOdd half map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Even half map

Fileemd_37465_half_map_2.map
AnnotationEven half map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Photosynthetic LH1-RC complex from the purple sulfur phototrophic...

EntireName: Photosynthetic LH1-RC complex from the purple sulfur phototrophic bacterium Allochromatium vinosum
Components
  • Complex: Photosynthetic LH1-RC complex from the purple sulfur phototrophic bacterium Allochromatium vinosum
    • Protein or peptide: Photosynthetic reaction center cytochrome c subunit
    • Protein or peptide: Reaction center protein L chainPhotosynthetic reaction centre
    • Protein or peptide: Reaction center protein M chainPhotosynthetic reaction centre
    • Protein or peptide: Photosynthetic reaction center H subunit
    • Protein or peptide: Antenna complex alpha/beta subunit
    • Protein or peptide: Antenna complex alpha/beta subunit
    • Protein or peptide: Antenna complex alpha/beta subunit
    • Protein or peptide: Antenna complex alpha/beta subunit
    • Protein or peptide: Antenna complex alpha/beta subunit
  • Ligand: PROTOPORPHYRIN IX CONTAINING FE
  • Ligand: MAGNESIUM ION
  • Ligand: (2S)-3-hydroxypropane-1,2-diyl dihexadecanoate
  • Ligand: PALMITIC ACID
  • Ligand: (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE
  • Ligand: BACTERIOCHLOROPHYLL ABacteriochlorophyll
  • Ligand: BACTERIOPHEOPHYTIN A
  • Ligand: Ubiquinone-8Coenzyme Q10
  • Ligand: DODECYL-BETA-D-MALTOSIDE
  • Ligand: CARDIOLIPIN
  • Ligand: FE (III) ION
  • Ligand: MENAQUINONE 8Vitamin K2
  • Ligand: SPIRILLOXANTHIN
  • Ligand: CALCIUM IONCalcium
  • Ligand: LAURYL DIMETHYLAMINE-N-OXIDE
  • Ligand: water

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Supramolecule #1: Photosynthetic LH1-RC complex from the purple sulfur phototrophic...

SupramoleculeName: Photosynthetic LH1-RC complex from the purple sulfur phototrophic bacterium Allochromatium vinosum
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#9
Source (natural)Organism: Allochromatium vinosum DSM 180 (bacteria)

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Macromolecule #1: Photosynthetic reaction center cytochrome c subunit

MacromoleculeName: Photosynthetic reaction center cytochrome c subunit / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Allochromatium vinosum DSM 180 (bacteria)
Molecular weightTheoretical: 41.512555 KDa
SequenceString: MNLGKQLTLP AVAVVASVVL LGCERPPPEV VQKGYRGVAM EQNYNPRLLE ASIKANLPVE SLPAAAPGGP SVSDVYENVQ VLKDLSVAE FTRTMVAVTT WVAPKEGCNY CHVPGNWASD DIYTKVVSRR MFELVRATNS NWKDHVAETG VTCYTCHRGN P VPKYVWVT ...String:
MNLGKQLTLP AVAVVASVVL LGCERPPPEV VQKGYRGVAM EQNYNPRLLE ASIKANLPVE SLPAAAPGGP SVSDVYENVQ VLKDLSVAE FTRTMVAVTT WVAPKEGCNY CHVPGNWASD DIYTKVVSRR MFELVRATNS NWKDHVAETG VTCYTCHRGN P VPKYVWVT DPGPNQPSGV TPTGQNYASS TVAYSALPLD PYTPFLDQSN EIRVIGQTAL PAGNTTSLKQ AEWTYGLMMQ IS DSLGVNC TFCHNSRSFY DWKQSTPQRT TAWYAIRHVR DINQNYIWPL NDALPASRKG PYGDPFKVGC MTCHQGAYKP LYG AQMAKD YPALYESAPA EAAPATEEAP AAEAEAVEAA PVEEAAPAPV EQAAAPVEDA APAPQQL

UniProtKB: Photosynthetic reaction center cytochrome c subunit

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Macromolecule #2: Reaction center protein L chain

MacromoleculeName: Reaction center protein L chain / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Allochromatium vinosum DSM 180 (bacteria)
Molecular weightTheoretical: 31.21741 KDa
SequenceString: MAMLSFERKY RVRGGTLIGG DLFDFWVGPF YVGFFGVAGF FFALLGVLLI VWGATIGPNA ELQTYNIWQI SIAPPDLSYG LGMAPMTEG GLWQIITICA IGAFVSWALR EVEICRKLGI GFHIPFAFAF AIGAYLVLVV VRPILMGAWG HGFPYGILSH L DWVSNVGY ...String:
MAMLSFERKY RVRGGTLIGG DLFDFWVGPF YVGFFGVAGF FFALLGVLLI VWGATIGPNA ELQTYNIWQI SIAPPDLSYG LGMAPMTEG GLWQIITICA IGAFVSWALR EVEICRKLGI GFHIPFAFAF AIGAYLVLVV VRPILMGAWG HGFPYGILSH L DWVSNVGY QFLHFHYNPA HMLAITFFFT NCLALSMHGS LILSVTNPQK GEEVKTSEHE NTFFRDIVGY SIGALAIHRL GL FLALSAV FWSAVCIVIS GPFWTRGWPE WWNWWLELPL W

UniProtKB: Reaction center protein L chain

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Macromolecule #3: Reaction center protein M chain

MacromoleculeName: Reaction center protein M chain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Allochromatium vinosum DSM 180 (bacteria)
Molecular weightTheoretical: 36.364242 KDa
SequenceString: MPEYQNIFTT VQVRAPAYPG VPLPKGSLPR IGKPIFSYWA GKIGDAQIGP IYLGFTGTLS IIFGFMAIFI IGFNMLASVD WNIIQFVKH FFWLGLEPPA PQYGLTIPPL SEGGWWLMAG FFLTMSILLW WVRTYKRAEA LGMSQHLSWA FAAAIFFYLS L GFIRPVMM ...String:
MPEYQNIFTT VQVRAPAYPG VPLPKGSLPR IGKPIFSYWA GKIGDAQIGP IYLGFTGTLS IIFGFMAIFI IGFNMLASVD WNIIQFVKH FFWLGLEPPA PQYGLTIPPL SEGGWWLMAG FFLTMSILLW WVRTYKRAEA LGMSQHLSWA FAAAIFFYLS L GFIRPVMM GSWAEAVPFG IFPHLDWTAA FSIRYGNLYY NPFHMLSIAF LYGSALLFAM HGATILAVSR FGGDREIDQI TD RGTAAER AAIFWRWTMG FNASMESIHR WAWWCAVLTV ITAGIGILLT GTVVENWYLW AIKHGVAPAY PEVVTAVDPY ATA TGVTQ

UniProtKB: Reaction center protein M chain

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Macromolecule #4: Photosynthetic reaction center H subunit

MacromoleculeName: Photosynthetic reaction center H subunit / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Allochromatium vinosum DSM 180 (bacteria)
Molecular weightTheoretical: 28.23132 KDa
SequenceString: (FME)SAAITEYMD VAQLTIWAFW FFFAGLIIYL RREDKREGYP LDSDRTERSG GRVKVVGFPD LAEPKTFVLP HNAGTV MAP RVEAPTSINA TPVAPFPGAP FEPNGDPMLS GFGPSASPDR AKHCDLTFEG LPKIVPLRVA TDFSIAERDP DPRGMTV VG LDGEVAGTVS ...String:
(FME)SAAITEYMD VAQLTIWAFW FFFAGLIIYL RREDKREGYP LDSDRTERSG GRVKVVGFPD LAEPKTFVLP HNAGTV MAP RVEAPTSINA TPVAPFPGAP FEPNGDPMLS GFGPSASPDR AKHCDLTFEG LPKIVPLRVA TDFSIAERDP DPRGMTV VG LDGEVAGTVS DVWVDRSEPQ IRYLEVKVAA GGKNVLLPIG FSRFDKKARK VKVAAIKAAH FANVPTLAKP DQITLYEE D KVCAYYAGGK LYATAERAGP LL

UniProtKB: Photosynthetic reaction center H subunit

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Macromolecule #5: Antenna complex alpha/beta subunit

MacromoleculeName: Antenna complex alpha/beta subunit / type: protein_or_peptide / ID: 5 / Number of copies: 9 / Enantiomer: LEVO
Source (natural)Organism: Allochromatium vinosum DSM 180 (bacteria)
Molecular weightTheoretical: 5.136131 KDa
SequenceString:
(FME)HKIWQIFDP RRTLVALFGF LFVLGLLIHF ILLSSPAFNW LSGS

UniProtKB: Antenna complex alpha/beta subunit

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Macromolecule #6: Antenna complex alpha/beta subunit

MacromoleculeName: Antenna complex alpha/beta subunit / type: protein_or_peptide / ID: 6 / Number of copies: 10 / Enantiomer: LEVO
Source (natural)Organism: Allochromatium vinosum DSM 180 (bacteria)
Molecular weightTheoretical: 5.269103 KDa
SequenceString:
MANSSMTGLT EQEAQEFHGI FVQSMTAFFG IVVIAHILAW LWRPWL

UniProtKB: Antenna complex alpha/beta subunit

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Macromolecule #7: Antenna complex alpha/beta subunit

MacromoleculeName: Antenna complex alpha/beta subunit / type: protein_or_peptide / ID: 7 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Allochromatium vinosum DSM 180 (bacteria)
Molecular weightTheoretical: 7.244583 KDa
SequenceString:
(FME)SPDLWKIWL LVDPRRILIA VFAFLTVLGL AIHMILLSTA EFNWLEDGVP AATVQQVTPV VPQR

UniProtKB: Antenna complex alpha/beta subunit

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Macromolecule #8: Antenna complex alpha/beta subunit

MacromoleculeName: Antenna complex alpha/beta subunit / type: protein_or_peptide / ID: 8 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Allochromatium vinosum DSM 180 (bacteria)
Molecular weightTheoretical: 5.518472 KDa
SequenceString:
MADQKSMTGL TEEEAKEFHG IFTQSMTMFF GIVIIAHILA WLWRPWL

UniProtKB: Antenna complex alpha/beta subunit

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Macromolecule #9: Antenna complex alpha/beta subunit

MacromoleculeName: Antenna complex alpha/beta subunit / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Allochromatium vinosum DSM 180 (bacteria) / Strain: DSM 180
Molecular weightTheoretical: 7.392857 KDa
SequenceString:
MMPQLYKIWL AFDPRMALIG LGAFLFALAL FIHYMLLRSP EFDWLLGPDY APVTLSAGMS ALPAGR

UniProtKB: Antenna complex alpha/beta subunit

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Macromolecule #10: PROTOPORPHYRIN IX CONTAINING FE

MacromoleculeName: PROTOPORPHYRIN IX CONTAINING FE / type: ligand / ID: 10 / Number of copies: 4 / Formula: HEM
Molecular weightTheoretical: 616.487 Da
Chemical component information

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

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Macromolecule #11: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 11 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #12: (2S)-3-hydroxypropane-1,2-diyl dihexadecanoate

MacromoleculeName: (2S)-3-hydroxypropane-1,2-diyl dihexadecanoate / type: ligand / ID: 12 / Number of copies: 1 / Formula: Z41
Molecular weightTheoretical: 568.911 Da
Chemical component information

ChemComp-Z41:
(2S)-3-hydroxypropane-1,2-diyl dihexadecanoate

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Macromolecule #13: PALMITIC ACID

MacromoleculeName: PALMITIC ACID / type: ligand / ID: 13 / Number of copies: 1 / Formula: PLM
Molecular weightTheoretical: 256.424 Da
Chemical component information

ChemComp-PLM:
PALMITIC ACID / Palmitic acid

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Macromolecule #14: (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-...

MacromoleculeName: (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE
type: ligand / ID: 14 / Number of copies: 11 / Formula: PGV
Molecular weightTheoretical: 749.007 Da
Chemical component information

ChemComp-PGV:
(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE / phospholipid*YM / Phosphatidylglycerol

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Macromolecule #15: BACTERIOCHLOROPHYLL A

MacromoleculeName: BACTERIOCHLOROPHYLL A / type: ligand / ID: 15 / Number of copies: 36 / Formula: BCL
Molecular weightTheoretical: 911.504 Da
Chemical component information

ChemComp-BCL:
BACTERIOCHLOROPHYLL A / Bacteriochlorophyll

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Macromolecule #16: BACTERIOPHEOPHYTIN A

MacromoleculeName: BACTERIOPHEOPHYTIN A / type: ligand / ID: 16 / Number of copies: 2 / Formula: BPH
Molecular weightTheoretical: 889.215 Da
Chemical component information

ChemComp-BPH:
BACTERIOPHEOPHYTIN A / Pheophytin

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Macromolecule #17: Ubiquinone-8

MacromoleculeName: Ubiquinone-8 / type: ligand / ID: 17 / Number of copies: 3 / Formula: UQ8
Molecular weightTheoretical: 727.109 Da
Chemical component information

ChemComp-UQ8:
Ubiquinone-8

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Macromolecule #18: DODECYL-BETA-D-MALTOSIDE

MacromoleculeName: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 18 / Number of copies: 22 / Formula: LMT
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

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Macromolecule #19: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 19 / Number of copies: 5 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

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Macromolecule #20: FE (III) ION

MacromoleculeName: FE (III) ION / type: ligand / ID: 20 / Number of copies: 1 / Formula: FE
Molecular weightTheoretical: 55.845 Da

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Macromolecule #21: MENAQUINONE 8

MacromoleculeName: MENAQUINONE 8 / type: ligand / ID: 21 / Number of copies: 1 / Formula: MQ8
Molecular weightTheoretical: 717.116 Da
Chemical component information

ChemComp-MQ8:
MENAQUINONE 8 / Vitamin K2

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Macromolecule #22: SPIRILLOXANTHIN

MacromoleculeName: SPIRILLOXANTHIN / type: ligand / ID: 22 / Number of copies: 17 / Formula: CRT
Molecular weightTheoretical: 596.925 Da
Chemical component information

ChemComp-CRT:
SPIRILLOXANTHIN

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Macromolecule #23: CALCIUM ION

MacromoleculeName: CALCIUM ION / type: ligand / ID: 23 / Number of copies: 6 / Formula: CA
Molecular weightTheoretical: 40.078 Da

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Macromolecule #24: LAURYL DIMETHYLAMINE-N-OXIDE

MacromoleculeName: LAURYL DIMETHYLAMINE-N-OXIDE / type: ligand / ID: 24 / Number of copies: 2 / Formula: LDA
Molecular weightTheoretical: 229.402 Da
Chemical component information

ChemComp-LDA:
LAURYL DIMETHYLAMINE-N-OXIDE / LDAO, detergent*YM / Lauryldimethylamine oxide

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Macromolecule #25: water

MacromoleculeName: water / type: ligand / ID: 25 / Number of copies: 334 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration4.0 mg/mL
BufferpH: 8
VitrificationCryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 277 K / Instrument: LEICA EM GP
DetailsThis sample was monodisperse.

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Electron microscopy

MicroscopeJEOL CRYO ARM 300
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.2 µm / Nominal defocus min: 0.7000000000000001 µm
Sample stageSpecimen holder model: JEOL / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Detector mode: COUNTING / Average exposure time: 2.0 sec. / Average electron dose: 50.0 e/Å2

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Image processing

Particle selectionNumber selected: 361654
Startup modelType of model: OTHER
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final 3D classificationSoftware - Name: RELION (ver. 3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final reconstructionNumber classes used: 1 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.24 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 252230
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

7vrj


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL / Protocol: RIGID BODY FIT / Overall B value: 44 / Target criteria: Correlation coefficient
Output model

PDB-8wdu:
Photosynthetic LH1-RC complex from the purple sulfur bacterium Allochromatium vinosum purified by sucrose density

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