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Yorodumi- EMDB-36198: Conformation 2 of intracellular domain of the plant potassium cha... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-36198 | |||||||||
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Title | Conformation 2 of intracellular domain of the plant potassium channel SKOR | |||||||||
Map data | ||||||||||
Sample |
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Keywords | voltage-gated potassium channel / outward-rectifier / symmetry reduction / ploy-ankyrin domain / PLANT PROTEIN | |||||||||
Function / homology | Function and homology information outward rectifier potassium channel activity / potassium ion homeostasis / monoatomic ion channel complex / potassium ion transport Similarity search - Function | |||||||||
Biological species | Arabidopsis thaliana (thale cress) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.0 Å | |||||||||
Authors | Liu S / Li S / Sun D / Tian C | |||||||||
Funding support | China, 2 items
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Citation | Journal: Cell Discov / Year: 2023 Title: Cryo-EM structure reveals a symmetry reduction of the plant outward-rectifier potassium channel SKOR. Authors: Siyu Li / Yuanxia Wang / Chenyang Wang / Yong Zhang / Demeng Sun / Peng Zhou / Changlin Tian / Sanling Liu / | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_36198.map.gz | 117.8 MB | EMDB map data format | |
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Header (meta data) | emd-36198-v30.xml emd-36198.xml | 13.3 KB 13.3 KB | Display Display | EMDB header |
Images | emd_36198.png | 83.6 KB | ||
Others | emd_36198_half_map_1.map.gz emd_36198_half_map_2.map.gz | 115.8 MB 115.8 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-36198 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-36198 | HTTPS FTP |
-Related structure data
Related structure data | 8jecC 8jetC 8jeuC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_36198.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.01 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_36198_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_36198_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Tetrameric SKOR complex
Entire | Name: Tetrameric SKOR complex |
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Components |
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-Supramolecule #1: Tetrameric SKOR complex
Supramolecule | Name: Tetrameric SKOR complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
-Macromolecule #1: Stellar K+ Outward Rectifier (SKOR) channel
Macromolecule | Name: Stellar K+ Outward Rectifier (SKOR) channel / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
Sequence | String: MGGSSGGGVS YRSGGESDVE LEDYEVDDFR DGIVESRGNR FNPLTNFLGL DFAGGSGGKF TVINGIRDI SRGSIVHPDN RWYKAWTMFI LIWALYSSFF TPLEFGFFRG LPENLFILDI A GQIAFLVD IVLTFFVAYR DSRTYRMIYK RSSIALRYLK STFIIDLLAC ...String: MGGSSGGGVS YRSGGESDVE LEDYEVDDFR DGIVESRGNR FNPLTNFLGL DFAGGSGGKF TVINGIRDI SRGSIVHPDN RWYKAWTMFI LIWALYSSFF TPLEFGFFRG LPENLFILDI A GQIAFLVD IVLTFFVAYR DSRTYRMIYK RSSIALRYLK STFIIDLLAC MPWDIIYKAA GE KEEVRYL LLIRLYRVHR VILFFHKMEK DIRINYLFTR IVKLIFVELY CTHTAACIFY YLA TTLPAS QEGYTWIGSL KLGDYSYSKF REIDLWTRYT TSMYFAVVTM ATVGYGDIHA VNMR EMIFA MVYISFDMIL GAYLIGNMTA LIVKGSKTER FRDKMADIMR YMNRNKLGRN IRGQI TGHL RLQYESSYTE AAVLQDIPVS IRAKIAQTLY LPYIEKVPLF RGCSSEFINQ IVIRLH EEF FLPGEVIMEQ GSVVDQLYFV CHGVLEEIGI TKDGSEEIVA VLQPDHSFGE ISILCNI PQ PYTVRVAELC RILRLDKQSF MNILEIFFHD GRRILNNLLE GKESNVRIKQ LESDITFH I SKQEAELALK LNSAAFYGDL YQLKSLIRAG GDPNKTDYDG RSPLHLAASR GYEDITLYL IQESVDVNIK DKLGSTPLLE AIKNGNDRVA ALLVKEGATL NIENAGTFLC TVVAKGDSDF LKRLLSNGI DPNSKDYDHR TPLHVAASEG FYVLAIQLVE ASANVLAKDR WGNTPLDEAL G CGNKMLIK LLEDAKNSQI SSFPSGSKEP KDKVYKKKCT VYFSHPGDSK EKRRRGIVLW VP RSIEELI RTAKEQLNVP EASCVLSEDE AKIIDVDLIS DGQKLYLAVE T UniProtKB: Potassium channel SKOR |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 56.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: INSILICO MODEL |
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Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 62707 |