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- EMDB-34511: SARS-CoV-2 RBD in complex with the repebody A6 (Focused Refinement) -

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Basic information

Entry
Database: EMDB / ID: EMD-34511
TitleSARS-CoV-2 RBD in complex with the repebody A6 (Focused Refinement)
Map data
Sample
  • Complex: SARS-CoV-2 Spike protein in complex with neutralizing repebody A6Coronavirus spike protein
KeywordsVIRAL PROTEIN
Biological speciesSevere acute respiratory syndrome coronavirus 2
Methodsingle particle reconstruction / cryo EM / Resolution: 4.0 Å
AuthorsKim UJ / Yoo Y / Cho HS
Funding support Korea, Republic Of, 1 items
OrganizationGrant numberCountry
National Research Foundation (NRF, Korea)NRF-2021R1A2C3010506 Korea, Republic Of
CitationJournal: To Be Published
Title: SARS-CoV-2 RBD in complex with the repebody A6 (Focused Refinement)
Authors: Kim UJ / Yoo Y / Cho HS
History
DepositionOct 18, 2022-
Header (metadata) releaseOct 25, 2023-
Map releaseOct 25, 2023-
UpdateOct 25, 2023-
Current statusOct 25, 2023Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_34511.png

Downloads & links

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Map

FileDownload / File: emd_34511.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.1834 Å
Density
Contour LevelBy AUTHOR: 0.22
Minimum - Maximum-13.264032 - 15.858224999999999
Average (Standard dev.)-0.0027689557 (±0.061000526)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 302.9504 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_34511_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_34511_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : SARS-CoV-2 Spike protein in complex with neutralizing repebody A6

EntireName: SARS-CoV-2 Spike protein in complex with neutralizing repebody A6Coronavirus spike protein
Components
  • Complex: SARS-CoV-2 Spike protein in complex with neutralizing repebody A6Coronavirus spike protein

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Supramolecule #1: SARS-CoV-2 Spike protein in complex with neutralizing repebody A6

SupramoleculeName: SARS-CoV-2 Spike protein in complex with neutralizing repebody A6
type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Severe acute respiratory syndrome coronavirus 2
Molecular weightTheoretical: 180 KDa

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.47 mg/mL
BufferpH: 8
Component:
ConcentrationFormulaName
200.0 mMNaClSodium chloridesodium chloride
20.0 mMC4H11NO3Tirs hydrochloride

Details: 20mM Tris-HCl, 200mM NaCl
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 12 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.6 µm / Nominal defocus min: 0.8 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 62.11 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.1.4)
Final 3D classificationNumber classes: 6 / Avg.num./class: 250000 / Software - Name: cryoSPARC (ver. 3.1.4)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.1.4)
Final reconstructionNumber classes used: 1 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 4.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.1.4) / Number images used: 350850
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT

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