+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-33012 | ||||||||||||
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Title | A CBg-ParM filament with GTP or GDPPi | ||||||||||||
Map data | Alp20 with GTP or GDPPi | ||||||||||||
Sample |
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Keywords | ParM actin-like plasmid segregation / CELL CYCLE | ||||||||||||
Function / homology | : Function and homology information | ||||||||||||
Biological species | Clostridium botulinum (bacteria) | ||||||||||||
Method | helical reconstruction / cryo EM / Resolution: 6.5 Å | ||||||||||||
Authors | Koh A / Ali S / Robinson R / Narita A | ||||||||||||
Funding support | Japan, 3 items
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Citation | Journal: To Be Published Title: A new actin-like filament from Clostridium botulinum exhibits a new system of filament turn over Authors: Koh A / Ali S / Popp D / Tanaka K / Kitaoku Y / Miyazaki N / Iwasaki K / Mitsuoka K / Robert R / Narita A | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_33012.map.gz | 9 MB | EMDB map data format | |
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Header (meta data) | emd-33012-v30.xml emd-33012.xml | 11.6 KB 11.6 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_33012_fsc.xml | 13.7 KB | Display | FSC data file |
Images | emd_33012.png | 40.1 KB | ||
Filedesc metadata | emd-33012.cif.gz | 5.4 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-33012 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-33012 | HTTPS FTP |
-Validation report
Summary document | emd_33012_validation.pdf.gz | 442.2 KB | Display | EMDB validaton report |
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Full document | emd_33012_full_validation.pdf.gz | 441.8 KB | Display | |
Data in XML | emd_33012_validation.xml.gz | 12 KB | Display | |
Data in CIF | emd_33012_validation.cif.gz | 16.3 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-33012 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-33012 | HTTPS FTP |
-Related structure data
Related structure data | 7x59MC 7x54C 7x55C 7x56C M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_33012.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | Alp20 with GTP or GDPPi | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.87 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : CBg-ParM filament with GTP or GDPPi
Entire | Name: CBg-ParM filament with GTP or GDPPi |
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Components |
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-Supramolecule #1: CBg-ParM filament with GTP or GDPPi
Supramolecule | Name: CBg-ParM filament with GTP or GDPPi / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Clostridium botulinum (bacteria) / Strain: Bf |
-Macromolecule #1: ParM/StbA family protein
Macromolecule | Name: ParM/StbA family protein / type: protein_or_peptide / ID: 1 / Number of copies: 5 / Enantiomer: LEVO |
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Source (natural) | Organism: Clostridium botulinum (bacteria) |
Molecular weight | Theoretical: 32.545352 KDa |
Recombinant expression | Organism: Escherichia coli BL21 (bacteria) |
Sequence | String: MKITVVDLGN INVKYVGENK GRFSSKITND YQSYEEGFQR VEYNGIKTYI GVGELSREFN KADRDYMAQL LYSLAKANTA DTKEINLTL LLPIIQMKNK TRLIETLKGE NFKFKFNGID REIKINDLMV LPEGYASYYS LDIENKKGDV CILDLGSRTI N ICVLENAK ...String: MKITVVDLGN INVKYVGENK GRFSSKITND YQSYEEGFQR VEYNGIKTYI GVGELSREFN KADRDYMAQL LYSLAKANTA DTKEINLTL LLPIIQMKNK TRLIETLKGE NFKFKFNGID REIKINDLMV LPEGYASYYS LDIENKKGDV CILDLGSRTI N ICVLENAK IVKTNTIKLG SFDFYSKIKS LENAKGEDYI EEDIQRLIDN GLIKVDSKQY IEFLSDILNA VKPYVNLKTY NT IFTGGTS LMLKEYIEKL PLNKFKVHPN ALTSNVDGAM EASKKVWN UniProtKB: UNIPROTKB: A0A6B3ZKE5 |
-Macromolecule #2: GUANOSINE-5'-TRIPHOSPHATE
Macromolecule | Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 2 / Number of copies: 5 / Formula: GTP |
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Molecular weight | Theoretical: 523.18 Da |
Chemical component information | ChemComp-GTP: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | helical reconstruction |
Aggregation state | filament |
-Sample preparation
Concentration | 0.7 mg/mL | ||||||||||||
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Buffer | pH: 7.5 Component:
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 298 K / Instrument: LEICA EM GP |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 45.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |