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- EMDB-32758: Cryo-EM structure of SARS-CoV-2 spike receptor-binding domain in ... -
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Open data
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Basic information
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Title | Cryo-EM structure of SARS-CoV-2 spike receptor-binding domain in complex with sea lion ACE2 | |||||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Li S / Han P / Qi J | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cross-species recognition and molecular basis of SARS-CoV-2 and SARS-CoV binding to ACE2s of marine animals. Authors: Shihua Li / Ruirui Yang / Di Zhang / Pu Han / Zepeng Xu / Qian Chen / Runchu Zhao / Xin Zhao / Xiao Qu / Anqi Zheng / Liang Wang / Linjie Li / Yu Hu / Rong Zhang / Chao Su / Sheng Niu / ...Authors: Shihua Li / Ruirui Yang / Di Zhang / Pu Han / Zepeng Xu / Qian Chen / Runchu Zhao / Xin Zhao / Xiao Qu / Anqi Zheng / Liang Wang / Linjie Li / Yu Hu / Rong Zhang / Chao Su / Sheng Niu / Yanfang Zhang / Jianxun Qi / Kefang Liu / Qihui Wang / George F Gao / ![]() Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has an extremely broad host range that includes hippopotami, which are phylogenetically closely related to whales. The cellular ACE2 ...Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has an extremely broad host range that includes hippopotami, which are phylogenetically closely related to whales. The cellular ACE2 receptor is one of the key determinants of the host range. Here, we found that ACE2s from several marine mammals and hippopotami could efficiently bind to the receptor-binding domain (RBD) of both SARS-CoV and SARS-CoV-2 and facilitate the transduction of SARS-CoV and SARS-CoV-2 pseudoviruses into ACE2-expressing cells. We further resolved the cryo-electron microscopy complex structures of the minke whale ACE2 and sea lion ACE2, respectively, bound to the RBDs, revealing that they have similar binding modes to human ACE2 when it comes to the SARS-CoV-2 RBD and SARS-CoV RBD. Our results indicate that marine mammals could potentially be new victims or virus carriers of SARS-CoV-2, which deserves further careful investigation and study. It will provide an early warning for the prospective monitoring of marine mammals. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 167.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.8 KB 13.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 12.5 KB | Display | ![]() |
Images | ![]() | 88.1 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7wshMC ![]() 7wseC ![]() 7wsfC ![]() 7wsgC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.669 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : SARS-CoV-2 spike receptor-binding domain in complex with sea lion ACE2
Entire | Name: SARS-CoV-2 spike receptor-binding domain in complex with sea lion ACE2 |
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Components |
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-Supramolecule #1: SARS-CoV-2 spike receptor-binding domain in complex with sea lion ACE2
Supramolecule | Name: SARS-CoV-2 spike receptor-binding domain in complex with sea lion ACE2 type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() ![]() ![]() |
-Supramolecule #2: SARS-CoV-2 spike receptor-binding domain
Supramolecule | Name: SARS-CoV-2 spike receptor-binding domain / type: complex / Chimera: Yes / ID: 2 / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() |
-Supramolecule #3: sea lion ACE2
Supramolecule | Name: sea lion ACE2 / type: complex / Chimera: Yes / ID: 3 / Parent: 1 / Macromolecule list: #2 |
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-Macromolecule #1: Angiotensin-converting enzyme
Macromolecule | Name: Angiotensin-converting enzyme / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: ![]() |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 93.009812 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MLGSSWLLLS LAALTAARST TEDLVKTFLE KFNSEAEELS YQSSLASWNY NTNITDENVQ KMNDAGAKWS AFYEEQSKQA KTYPLEEIQ DSTVKRQLQA LQHSGSSVLS ADKSQRLNTI LNAMSTIYST GKACNPNNPQ ECLLLEPGLD DIMANSRDYN E RLWAWEGW ...String: MLGSSWLLLS LAALTAARST TEDLVKTFLE KFNSEAEELS YQSSLASWNY NTNITDENVQ KMNDAGAKWS AFYEEQSKQA KTYPLEEIQ DSTVKRQLQA LQHSGSSVLS ADKSQRLNTI LNAMSTIYST GKACNPNNPQ ECLLLEPGLD DIMANSRDYN E RLWAWEGW RSEVGKQLRP LYEEYVALKN EMARANNYED YGDYWRGDYE EEWTNGYNYS RDQLIKDVEQ TFTQIQPLYE HL HAYVRAK LMDTYPSHMS PTGCLPAHLL GDMWGRFWTN LYPLTVPFGQ KPNIDVTDTM VNQSWDARRI FEEAEKFFVS VGL PNMTQG FWENSMLTEP GDSRKVVCHP TAWDLGKHDF RIKMCTKVTM DDFLTAHHEM GHIQYDMAYA AQPFLLRNGA NEGF HEAVG EIMSLSAATP KHLKNIGLLP PGFSEDNETD INFLFKQALT IVGTLPFTYM LEKWRWMVFK GEIPKEQWMK KWWEM KRDL VGVVEPLPHD ETYCDPASLF HVANDYSFIR YYTRTIYQFQ FQEALCQIAK HEGPLHKCDI SNSSEAGQTL LQMLKL GRS KPWTLALYRV VGAKNMDVRP LLNYFDPLFT WLKEQNRNSF VGWNTDWSPY ADQSIKVRIS LKSALGEKAY EWNDNEM YL FRSSIAYAMR EYFSKVKNQM IPFVEDNVWV NNLKPRISFT FFVTSPGNMS DIIPRADVEE AIRMSRGRIN DAFRLDDN S LEFLGIQPTL EPPYQPPVTI WLIVFGVVMA VVVVGIVLLI FSGIRSRRKN DQATSEENPY ASVNLSKGEN NPGFQNVDD VQTSSF |
-Macromolecule #2: Spike protein S1
Macromolecule | Name: Spike protein S1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 21.873496 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: TNLCPFGEVF NATRFASVYA WNRKRISNCV ADYSVLYNSA SFSTFKCYGV SPTKLNDLCF TNVYADSFVI RGDEVRQIAP GQTGKIADY NYKLPDDFTG CVIAWNSNNL DSKVGGNYNY LYRLFRKSNL KPFERDISTE IYQAGSTPCN GVEGFNCYFP L QSYGFQPT ...String: TNLCPFGEVF NATRFASVYA WNRKRISNCV ADYSVLYNSA SFSTFKCYGV SPTKLNDLCF TNVYADSFVI RGDEVRQIAP GQTGKIADY NYKLPDDFTG CVIAWNSNNL DSKVGGNYNY LYRLFRKSNL KPFERDISTE IYQAGSTPCN GVEGFNCYFP L QSYGFQPT NGVGYQPYRV VVLSFELLHA PATVCGP |
-Macromolecule #3: ZINC ION
Macromolecule | Name: ZINC ION / type: ligand / ID: 3 / Number of copies: 1 / Formula: ZN |
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Molecular weight | Theoretical: 65.409 Da |
-Macromolecule #4: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 4 / Number of copies: 1 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |