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- EMDB-32344: Structure of Mammalian NALCN-FAM155A-UNC79-UNC80 quanternary complex -

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Basic information

Entry
Database: EMDB / ID: EMD-32344
TitleStructure of Mammalian NALCN-FAM155A-UNC79-UNC80 quanternary complex
Map dataNALCN-FAM155A-UNC79-UNC80 complex
Sample
  • Complex: NALCN-FAM155A-UNC79-UNC80 complex
    • Protein or peptide: Protein unc-79 homolog
    • Protein or peptide: Protein unc-80 homolog
    • Protein or peptide: Sodium leak channel non-selective protein
    • Protein or peptide: Transmembrane protein FAM155ATransmembrane protein
  • Ligand: ZINC ION
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
Function / homology
Function and homology information


monoatomic cation homeostasis / positive regulation of synaptic transmission, cholinergic / leak channel activity / regulation of resting membrane potential / cation channel complex / voltage-gated monoatomic ion channel activity / Stimuli-sensing channels / voltage-gated sodium channel activity / regulation of monoatomic ion transmembrane transport / calcium ion import across plasma membrane ...monoatomic cation homeostasis / positive regulation of synaptic transmission, cholinergic / leak channel activity / regulation of resting membrane potential / cation channel complex / voltage-gated monoatomic ion channel activity / Stimuli-sensing channels / voltage-gated sodium channel activity / regulation of monoatomic ion transmembrane transport / calcium ion import across plasma membrane / sodium ion transmembrane transport / monoatomic cation channel activity / potassium ion transmembrane transport / positive regulation of synaptic transmission, GABAergic / calcium ion transmembrane transport / axon / plasma membrane
Similarity search - Function
NALCN channel auxiliary factor 1/2 / UNC79 / Cation channel complex component UNC80, N-terminal / Protein UNC80, central region / UNC80 N-terminal / Protein UNC80 central region / Sodium leak channel NALCN / Voltage-dependent channel domain superfamily / Ion transport domain / Ion transport protein
Similarity search - Domain/homology
Protein unc-80 homolog / Protein unc-79 homolog / Sodium leak channel NALCN / NALCN channel auxiliary factor 1
Similarity search - Component
Biological speciesMus musculus (house mouse) / Rattus norvegicus (Norway rat)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsChen L / Kang Y
Funding support China, 4 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China)2106YFA0502004 China
National Natural Science Foundation of China (NSFC)31622021 China
National Natural Science Foundation of China (NSFC)31821091 China
National Natural Science Foundation of China (NSFC)31870833 China
CitationJournal: Nat Commun / Year: 2022
Title: Structure and mechanism of NALCN-FAM155A-UNC79-UNC80 channel complex.
Authors: Yunlu Kang / Lei Chen /
Abstract: NALCN channel mediates sodium leak currents and is important for maintaining proper resting membrane potential. NALCN and FAM155A form the core complex of the channel, the activity of which ...NALCN channel mediates sodium leak currents and is important for maintaining proper resting membrane potential. NALCN and FAM155A form the core complex of the channel, the activity of which essentially depends on the presence of both UNC79 and UNC80, two auxiliary proteins. NALCN, FAM155A, UNC79, and UNC80 co-assemble into a large hetero-tetrameric channel complex. Genetic mutations of NALCN channel components lead to neurodevelopmental diseases. However, the structure and mechanism of the intact channel complex remain elusive. Here, we present the cryo-EM structure of the mammalian NALCN-FAM155A-UNC79-UNC80 quaternary complex. The structure shows that UNC79-UNC80 form a large piler-shaped heterodimer which was tethered to the intracellular side of the NALCN channel through tripartite interactions with the cytoplasmic loops of NALCN. Two interactions are essential for proper cell surface localization of NALCN. The other interaction relieves the self-inhibition of NALCN by pulling the auto-inhibitory CTD Interacting Helix (CIH) out of its binding site. Our work defines the structural mechanism of NALCN modulation by UNC79 and UNC80.
History
DepositionDec 4, 2021-
Header (metadata) releaseMay 18, 2022-
Map releaseMay 18, 2022-
UpdateDec 7, 2022-
Current statusDec 7, 2022Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_32344.png

Downloads & links

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Map

FileDownload / File: emd_32344.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationNALCN-FAM155A-UNC79-UNC80 complex
Voxel sizeX=Y=Z: 1.324 Å
Density
Contour LevelBy AUTHOR: 0.95
Minimum - Maximum-0.1394773 - 8.606241
Average (Standard dev.)0.08691605 (±0.1552146)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions280280280
Spacing280280280
CellA=B=C: 370.72 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : NALCN-FAM155A-UNC79-UNC80 complex

EntireName: NALCN-FAM155A-UNC79-UNC80 complex
Components
  • Complex: NALCN-FAM155A-UNC79-UNC80 complex
    • Protein or peptide: Protein unc-79 homolog
    • Protein or peptide: Protein unc-80 homolog
    • Protein or peptide: Sodium leak channel non-selective protein
    • Protein or peptide: Transmembrane protein FAM155ATransmembrane protein
  • Ligand: ZINC ION
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose

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Supramolecule #1: NALCN-FAM155A-UNC79-UNC80 complex

SupramoleculeName: NALCN-FAM155A-UNC79-UNC80 complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4
Source (natural)Organism: Mus musculus (house mouse)

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Macromolecule #1: Protein unc-79 homolog

MacromoleculeName: Protein unc-79 homolog / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 297.597562 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MSTKAEQFAS KIRYLQEYHN RVLHNIYPVP SGTDIANTLK YFSQTLLSVL RDAPSERGPQ SRDAQLSDYP SLDYQGLYVT LVTLLDLVP LLQHGQHDLG QSIFYTTTCL LPFLNDDVLS TLPYTMISTL ATFPPFLHKD IIEYLSTSFL PMAILGSSGR E GVPAHVNL ...String:
MSTKAEQFAS KIRYLQEYHN RVLHNIYPVP SGTDIANTLK YFSQTLLSVL RDAPSERGPQ SRDAQLSDYP SLDYQGLYVT LVTLLDLVP LLQHGQHDLG QSIFYTTTCL LPFLNDDVLS TLPYTMISTL ATFPPFLHKD IIEYLSTSFL PMAILGSSGR E GVPAHVNL SASSMLMIAM QYTSNPVYHC QLLECLMKYK QEVWKDLLYV IAYGPSQVKP PAVQMLFHYW PNLKPPGAIS EY RGLQYTA WNPIHCQHIE CHNAINKPAV KMCIDPSLSV ALGDKPPPLY LCEECSERIS GDHSEWLIDV LLPQAEISAI CQK KNCSSH VRRAVVTCFS AGCCGRHGNR PVRYCKRCHS NHHSNEVGAT AETHLYQTSP PPINTRECGA EELVCAVEAV ISLL KEAEF HAEQREHELN RRRQLGLSSS HHSLDNTDFD NKDDDKHDQR LLSQFGIWFL VSLCTPSENT PTESLARLVA MVFQW FHST AYMMDDEVGS LVEKLKPQFV TKWLKTVCDV RFDVMVMCLL PKPMEFARVG GYWDKSCSTV TQLKEGLNRI LCLIPY NVI SQSVWECIMP EWLEAIRTEV PDNQLKEFRE VLSKMFDIEL CPLPFSMEEM FGFISCRFTG YPSTVQEQAL LWLHVLS EL DITVPLQLLI SMFSDGVNSV KELANQRKSR ANELAGNLAS RRVSVASDPG RRGQHNTLSP FHSPFQSPFR SPMRSPFR S PFKNFGHPGG RTIDFDCEDD DMNLNCFILM FDLLLKQMEL QDDGITMGLE HSLSKDIISI INNVFQAPWG GSHSCQKDK KATECNLCQS SILCYQLACE LLERLAPKEE SRLVEPTDSL EDSLLSSRPE FILGPEGEEE ENPAAKHGEN PGNRTVPSEH AAIKNDTER KFCYQQLPVT LRLIYTIFQE MAKFEEPDIL FNMLNCLKIL CLHGECLYTA RKDHPQFLAY IQDHMLIASL W RVVKSEFS QLSSLAVPLL LHALSLPHGA DIFWTIINGN FNSKDWKMRF EAVEKVAVIC RFLDIHSVTK NHLLKYSLAH AF CCFLTAV EDVNPAVATR AGLLLDTIKR PALQGLCLCL DFQFDTVVKD RPTILSKLLL LHFLKQDIPA LSWEFFVNRF ETL SLEAQL HLDCNKEFPF PTTITAVRTN VANLSDAALW KIKRARFARN RQKSVRSLRD SVKGPAESKR ALSLPETLTS KIPV RLTRH EQSAPALGGT PEQTPGQQSP ENDNTIKDLL PEDAGIDHQT VHQLITVLMK FMARDESSAE SDISSAKAFN TVKRH LYVL LGYDQQEGCF MIAPQKMRLS TCFNAFIAGI AQVMDYNINL GKHLLPLVVQ VLKYCSCPQL RHYFQQPPRC SLWSLK PHI RQMWLKALLV ILYKYPYRDC DVSKTLLHLI HITVNTLNAQ YHSCKPHATA GPLYTDNSNI SRYSEKEKEE DSVFDES DI HDTPTGPCNK ESQTFFARLK RIGGSRMVKG QPVGMNVQRS EIELAEYRET GALQDSVLHC VREESIQKKK LRSLKQKS L DIGNADSLLF TLDEHRRKSC IDRCDIDKPP AQAAYISQRQ NDHHGRSRQN SATRPDNTEI PKNPGTEGFQ EIRRPVIPE VRLNCMETFE VRVDSPGKPA PREDLDLIDL SSDSTSGPEK HSILSTSDSD SLVFEPLPPL RIVESDEEEE MMNQGNGGAL GNNAASSPS IPSQPSVLSL STTPLVQVSV EDCSKDFSSK DSGNHQSASN EDSTIAALDD LTDSEELSKS EELREFASGS P LTLKQKRD LLQKSSAVPE MSVDYNPEPS PAEEKPGQTP TSGVKTVLLK VPEDGENLIE SEKPNTSAES DTEQNPERKV EE DGAEESE FKIQIVPRQR KQRKIAVSAI QREYLDISFN ILDKLGEQKD PDPSAKGLST LEMPRESSSA PTLEAGAPET SSH SSISTQ YRQMKRGSLG VLTMSQLMKR QLEHQSSAPH NISSWDTEQI QPGKRQCNVP MCLNPDLEGQ PLRTRGATKS SLLS APSIA SMFVPAPEEF TEEQPTVMAD KCHDCGAILE EYDEETLGLA IVVLSTFIHL SPDLAAPLLL DIMQSVGRLA SSTTF SNQA ESMMVPGNAA GVAKQFLRCI FHQLAPNGIF PQLFQSAIKD GTFLRTLATS LMDFNELSSI AALSQLLEGL NNKKNL PAG GAMIRCLENI ATFMEALPMD SPSSLWTTIS NQFQTFFAKL PCVLPLKCSL DSSLRIMICL LKIPSTNATR SLLEPFS KL LSFVIQNAVF TLAYLVELCG LCYRAFTKER DKFYLSRSVV LELLQALKLK SPLPDTNLLL LVQFICADAG TKLAESTI L SKQMIASVPG CGTAAMECIR QYVSEVLEFM ADMHTLTKLK SHMKTCSQPL HEDTFGGHLK VGLAQIAAME ISRGNHRDN KAVIRYLPWL YHPPSAMQQG PKEFIECVSH IRLLSWLLLG SLTHNAVCPN ASSPCLPIPL DAGSHIADHL IVILIGFPEQ SKTCVLHMC SLFHAFIFAQ LWTVYCEQSA VATNVQNQNE FSFTAILTAL EFWSRVTPSI LQLMAHNKVM VEMVCLHVIS L MEALQECN STIFVKLIPM WLPMIQSNTK HLSAGLQLRL QAIQNNVNHH SLRTLPGSGQ SSAGLAALRK WLQCTQFKMA QV EIQSSEA ASQFYPL

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Macromolecule #2: Protein unc-80 homolog

MacromoleculeName: Protein unc-80 homolog / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 371.044062 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MVKRKSSEGQ EQDGGRGIPL PIQTFLWRQT SAFLRPKLGK QYEASCVSFE RVLVENKLHG LSPALSEAIQ SISRWELVQA ALPHVLHCT ATLLSNRNKL GHQDKLGVAE TKLLHTLHWM LLEAPQDCNN DQFGGTDRGS SWGGSSSAFI HQIENQGSPG Q PCRSSSHD ...String:
MVKRKSSEGQ EQDGGRGIPL PIQTFLWRQT SAFLRPKLGK QYEASCVSFE RVLVENKLHG LSPALSEAIQ SISRWELVQA ALPHVLHCT ATLLSNRNKL GHQDKLGVAE TKLLHTLHWM LLEAPQDCNN DQFGGTDRGS SWGGSSSAFI HQIENQGSPG Q PCRSSSHD EEENNRRKTF QNSMATVELF VFLFAPLVHR IKESDLTFRL ASGLVIWQPM WEHRQPEVSG FTALVKPIRN II TAKRSSP INSQSQTCES PNQDTRQQGE GLQVVSEALQ SDSISPKATI SGCHQGNSFD GSLSSQTSQE RGPSHSRASL VIP PCQRSR YATYFDVAVL RCLLQPHWSE EGTQWSLMYY LQRLRHMLEE KPEKTPDPDI PLLPRPRSSS MVAAAPSLVN THKT QDLTM KCNEEEKSLS PEAFSKVSLT NLRRSAVPDL SSDLGMNIFK KFKSRKEDRE RKGSIPFHHT GKRRPRRMGV PFLLH EDHL DVSPTRSTFS FGSFSGLGED RRGIEKGGWQ TTILGKLTRR GSSDAATEME SLSARHSHSH HTLVSDLPDH SNSHGE NTV KEVRSQISTI TVATFNTTLA SFNVGYADFF SEHMRKLCSQ VPIPEMPHEP LACANLPRSL TDSCINYSYL EDTEHID GT NNFVHKNGML DLSVVLKAVY LVLNHDISSR ICDVALNIVE CLLQLGVVPC VEKNRKKSEN KENESVEKRP SEGAFQFK G VSSSSTSGFG APSASGAGDG GGEEGGGGDG GGGGGGGDGG GGGGGGGGPY EKNEKNQEKD DNIPVSNHRL ALTMLIKIV KSLGCAYGCG EGHRGLSGDR LRHQVFRENA QNCLTKLYKL DKIQFRQTMR DYVNKDSLNN VVDFLHALLG FCMEPVTDNK AGFGNNFTT VDNKSTAQNV EGIIVGAMFK SLITRCASTT HELHSPENLG LYCDIRQLVQ FIKEAHGNVF RRVALSALLD S AEKLAPGK KVEENGQESK PVGSKRSEAG SIADKGQVSS APEECRSFMS GRPSQTPEHD EPMQGGNLGR KDFWRKMFKS QS AASDTSS QSEQDTSECT TAHSGNTSDR RARSRSRRIS LRKKLKLPIG NWLKRSSLSG LADGVEDLLD ISSVDRLSFI RQS SKVKFT SAVKLSEGGP GSGMENGREE EENFFKRLGC HSFDDHLSPN QDGGKSKNVV NLGAIRQGMK RFQFLLNCCE PGTI PDASI LAAALDLEAP VVARAALFLE CARFVHRCNR GNWPEWMKGH HVNITKKGLS RGRSPTVGNK RNQKLQWSAA KLFYQ WGDA IGIRLNELCH GESESPANLL GLIYDEETKR RLRKEDEEED FLDDSTVNPS KCGCPFALKM AACQLLLEIT TFLRET FSC LPRPRTEPLV DLESCRLRLD PELDRHRYER KISFAGVLDE NEDSKDSLHS SSHTIKSDAG AEEKKVPSRK IRIGGSR LL QIKGTRSFQV KKGGSLSSIR RVGSLKSSKL SRQDSESEAE ELQLSQSRDT VTDLEGSPWS ASEPSIEPEG LSNAGTEE N YHRNMSWLHV MILLCNQQSF ICTHVDYCHP HCYLHHSRSC ARLVRAIKLL YGDSVDSLRE SNHISNVALR GKKQKECSD KSCLRTPSLK KRVSDVNLEG KKDSGMLKYI RFQVMSLSPA PLSLLIKAAP ILTEEMYGDI QPAAWELLLS MDEHMAGAAA AMFLLCAVK VPDAVSDMLM SEFHHAETVQ RLNAVLKFHT LWRFRYQVWP RMEEGAQQIF KIPPPSINFT LPSPVLGMPS V PMFDPPWV PQCSGSVQDP INEDQSKSFS ARAVSRSHQR AEHILKNLQQ EEEKKRLGRE ASLITAIPIT QEACYEPTCT PN SEPEEEE EVANLTSRRL SVSPSCTSST SHRNYSFRRG SVWSVRSAVS AEDEEHATEH TPNHHVPQPP QAVFPACICA AVL PIVHLM EDGEVREDGV AVSAVAQQVL WNCLIEDPST VLRHFLEKLT ISNRQDELMY MLRKLLLNIG DFPAQTSHIL FNYL VGLIM YFVRTPCEWG MDAISATLTF LWEVVGYVEG LFFKDLKQTM KKEQCEVKLL VTASMPGTKT LVVHGQNECD IPTQL PVHE DTQFEALLKE CLEFFNIPES QSTHYFLMDK RWNLIHYNKT YVRDIYPFRR SVSPQLNLVH MHPEKGQELI QKQVFT RKL EEVGRVLFLI SLTQKIPTAH KQSHVSMLQE DLLRLPSFPR SAIDAEFSLF SDPQAGKELF GLDTLQKSLW IQLLEEM FL GMPSEFPWGD EIMLFLNVFN GALILHPEDS ALLRQYAATV INTAVHFNHL FSLSGYQWIL PTMLQVYSDY ESNPQLRR A IEFACHQFYI LHRKPFVLQL FASVAPLLEF PDAANTGSSK GVSAQCLFDL LQSLEGETTD ILDILELVKA EKPLKSLDF CYGNEDLTFS ISEAIKLCVT VVAYAPESFR SLQMLMVLEA LVPCYLQKMK RQTSQVETVP AAREEIAATA ALATSLQALL YSVEVLTRP MTAPQMSRSD QGHKGTTTAN HTMSSGVNTR YPEQGAKLHF IRENLHLLEE GQGLPREELD ERISREEFRR P RESLLNIC TEFYKHCGPR LKILQNLAGE PRVTALELLD VKSHMRLAEI AHSLLKLAPY DTQTMESRGL RRYIMEMLPI TD WSAEAVR PALILILKRL DRMFNKIHKM PTLRRQVEWE PASSLIEGVC LTLQRQPIIS FLPHLRSLIN VCVNLVMGVV GPS SVADGL PLLHLSPYLS PPLPFSTAVV RLVALQIQAL KEDFPLSHVI SPFTNQERRE GMLLNLLIPF VLTVGSGSKD SPWL EQPEV QLLLQTVINV LLPPRIISTS RSKNFMLESS PAHCSTPGDA GKDLRKEGLA ESTSQAAYLA LKVILVCFER QLGSQ WYWL SLQVKEMALR KVGGLALWDF LDFIVRTRIP IFVLLRPFIQ CKLLAQPAEN HEELSARQHI SDQLERRFIP RPLCKS SLI AEFNSELKIL KEAVHSGSAY QGKTSISTVG TSTSAYRLSL ATMSRSNTGT GTVWEQDSEP SQQASQDTLS RTDEEDE EN DSVSMPSVVS EQEACLLSTI GRRRFSSHVS SMSAPQAEVG MLPSQSEPNV LDDSQGLAAE GSLSRVASIQ SEPGQQNV L LQQPLGRKRG LRQLRRPLLS RQKTQTEPRN RHGARLSTTR RSIQPKTKPS VDQKRSVTFI EAQPEPTAAP TDIFPATGQ PQSCSPGRAR KPEGTEKPVL TSSPAIIIAD LHSLSPKQSE PLLAEEGEKK EDEEIQGATA HCPLSTQLSD PDDFTGLETS SLLQHGDTV LHISEENGTE NPLLSSQFTF TPPELGDTDS ALDESHV

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Macromolecule #3: Sodium leak channel non-selective protein

MacromoleculeName: Sodium leak channel non-selective protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rattus norvegicus (Norway rat)
Molecular weightTheoretical: 200.682031 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MLKRKQSSRV EAQPVTDFGP DESLSDNADI LWINKPWVHS LLRICAIISV ISVCMNTPMT FEHYPPLQYV TFTLDTLLMF LYTAEMIAK MHIRGIVKGD SSYVKDRWCV FDGFMVFCLW VSLVLQVFEI ADIVDQMSPW GMLRIPRPLI MIRAFRIYFR F ELPRTRIT ...String:
MLKRKQSSRV EAQPVTDFGP DESLSDNADI LWINKPWVHS LLRICAIISV ISVCMNTPMT FEHYPPLQYV TFTLDTLLMF LYTAEMIAK MHIRGIVKGD SSYVKDRWCV FDGFMVFCLW VSLVLQVFEI ADIVDQMSPW GMLRIPRPLI MIRAFRIYFR F ELPRTRIT NILKRSGEQI WSVSIFLLFF LLLYGILGVQ MFGTFTYHCV VNDTKPGNVT WNSLAIPDTH CSPELEEGYQ CP PGFKCMD LEDLGLSRQE LGYSGFNEIG TSIFTVYEAS SQEGWVFLMY RAIDSFPRWR SYFYFITLIF FLAWLVKNVF IAV IIETFA EIRVQFQQMW GTRSSTTSTA TTQMFHEDAA GGWQLVAVDV NKPQGRAPAC LQKMMRSSVF HMFILSMVTV DVIV AASNY YKGENFRRQY DEFYLAEVAF TVLFDLEALL KIWCLGFTGY ISSSLHKFEL LLVIGTTLHV YPDLYHSQFT YFQVL RVVR LIKISPALED FVYKIFGPGK KLGSLVVFTA SLLIVMSAIS LQMFCFVEEL DRFTTFPRAF MSMFQILTQE GWVDVM DQT LNAVGHMWAP LVAIYFILYH LFATLILLSL FVAVILDNLE LDEDLKKLKQ LKQSEANADT KEKLPLRLRI FEKFPNR PQ MVKISKLPSD FTVPKIRESF MKQFIDRQQQ DTCCLFRILP STSSSSCDNP KRPTVEDNKY IDQKLRKSVF SIRARNLL E KETAVTKILR ACTRQRMLSG SFEGQPAKER SILSVQHHIR QERRSLRHGS NSQRISRGKS LETLTQDHSN TVRYRNAQR EDSEIKMIQE KKEQAEMKRK VQEEELRENH PYFDKPLFIV GREHRFRNFC RVVVRARFNA SKTDPVTGAV KNTKYHQLYD LLGLVTYLD WVMITVTICS CISMMFESPF RRVMHAPTLQ IAEYVFVIFM SIELNLKIMA DGLFFTPTAV IRDFGGVMDI F IYLVSLIF LCWMPQNVPA ESGAQLLMVL RCLRPLRIFK LVPQMRKVVR ELFSGFKEIF LVSILLLTLM LVFASFGVQL FA GKLAKCN DPNIIRREDC NGIFRINVSV SKNLNLKLRP GEKKPGFWVP RVWANPRNFN FDNVGNAMLA LFEVLSLKGW VEV RDVIIH RVGPIHGIYI HVFVFLGCMI GLTLFVGVVI ANFNENKGTA LLTVDQRRWE DLKSRLKIAQ PLHLPPRPDN DGFR AKMYD ITQHPFFKRT IALLVLAQSV LLSVKWDVED PVTVPLATMS VVFTFIFVLE VTMKIIAMSP AGFWQSRRNR YDLLV TSLG VVWVVLHFAL LNAYTYMMGA CVIVFRFFSI CGKHVTLKML LLTVVVSMYK SFFIIVGMFL LLLCYAFAGV VLFGTV KYG ENINRHANFS SAGKAITVLF RIVTGEDWNK IMHDCMVQPP FCTPDEFTYW ATDCGNYAGA LMYFCSFYVI IAYIMLN LL VAIIVENFSL FYSTEEDQLL SYNDLRHFQI IWNMVDDKRE GVIPTFRVKF LLRLLRGRLE VDLDKDKLLF KHMCYEME R LHNGGDVTFH DVLSMLSYRS VDIRKSLQLE ELLAREQLEY TIEEEVAKQT IRMWLKKCLK RIRAKQQQSC SIIHSLRES QQQELSRFLN PPSIETTQPS EDTNANSQDH NTQPESSSQQ QLLSPTLSDR GGSRQDAADT GKPQRKIGQW RLPSAPKPIS HSVSSVNLR FGGRTTMKSV VCKMNPMPDT ASCGSEVKKW WTRQLTVESD ESGDDLLDI

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Macromolecule #4: Transmembrane protein FAM155A

MacromoleculeName: Transmembrane protein FAM155A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 52.795801 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MTRGAWMCRQ YDDGLKIWLA APRENEKPFI DSERAQKWRL SLASLLFFTV LLSDHLWFCA EAKLTRTRDK EHHQQQQQQQ QQQQQQQQQ QQQQQQRQQQ RQRQQQRQRQ QEPSWPALLA SMGESSPAAQ AHRLLSASSS PTLPPSPGGG GGSKGNRGKN N RSRALFLG ...String:
MTRGAWMCRQ YDDGLKIWLA APRENEKPFI DSERAQKWRL SLASLLFFTV LLSDHLWFCA EAKLTRTRDK EHHQQQQQQQ QQQQQQQQQ QQQQQQRQQQ RQRQQQRQRQ QEPSWPALLA SMGESSPAAQ AHRLLSASSS PTLPPSPGGG GGSKGNRGKN N RSRALFLG NSAKPVWRLE TCYPQGASSG QCFTVESADA VCARNWSRGA AAGEEQSSRG SRPTPLWNLS DFYLSFCNSY TL WELFSGL SSPSTLNCSL DVVLTEGGEM TTCRQCIEAY QDYDHHAQEK YEEFESVLHK YLQSDEYSVK SCPEDCKIVY KAW LCSQYF EVTQFNCRKT IPCKQYCLEV QTRCPFILPD NDEVIYGGLS SFICTGLYET FLTNDEPECC DIRSEEQTAP RPKG TVDRR DSCPRTSLTV SSATRLCPGR LKLCVLVLIL LHTVLTASAA QNSTGLGLGG LPTLEDNSTR ED

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Macromolecule #5: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 5 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #6: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 6 / Number of copies: 3 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.5 µm
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 50.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 275170

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