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Yorodumi- EMDB-32034: Structure of unmodified E. hirae V-type ATPase using Volta phase plate -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-32034 | |||||||||
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Title | Structure of unmodified E. hirae V-type ATPase using Volta phase plate | |||||||||
Map data | E. hirae V-ATPase (native type) | |||||||||
Sample |
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Biological species | Enterococcus hirae (bacteria) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 6.1 Å | |||||||||
Authors | Burton-Smith RN / Murata K | |||||||||
Funding support | Japan, 1 items
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Citation | Journal: To Be Published Title: Examining E. hirae V-ATPase by Zernike and Volta phase plate cryo-EM Authors: Burton-Smith RN / Murata K | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_32034.map.gz | 112.3 MB | EMDB map data format | |
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Header (meta data) | emd-32034-v30.xml emd-32034.xml | 7.1 KB 7.1 KB | Display Display | EMDB header |
Images | emd_32034.png | 50.1 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-32034 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-32034 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_32034.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | E. hirae V-ATPase (native type) | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.113 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : Recombinant, unmodified E. hirae V-ATPase by Volta phase plate
Entire | Name: Recombinant, unmodified E. hirae V-ATPase by Volta phase plate |
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Components |
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-Supramolecule #1: Recombinant, unmodified E. hirae V-ATPase by Volta phase plate
Supramolecule | Name: Recombinant, unmodified E. hirae V-ATPase by Volta phase plate type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Enterococcus hirae (bacteria) |
Recombinant expression | Organism: Escherichia coli (E. coli) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy |
Specialist optics | Phase plate: VOLTA PHASE PLATE |
Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: OTHER / Details: Ab initio |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 6.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 73711 |