Japan Agency for Medical Research and Development (AMED)
JP19am0101117, JP17pc0101020
Japan
Citation
Journal: Nat Commun / Year: 2021 Title: Structure of the molecular bushing of the bacterial flagellar motor. Authors: Tomoko Yamaguchi / Fumiaki Makino / Tomoko Miyata / Tohru Minamino / Takayuki Kato / Keiichi Namba / Abstract: The basal body of the bacterial flagellum is a rotary motor that consists of several rings (C, MS and LP) and a rod. The LP ring acts as a bushing supporting the distal rod for its rapid and stable ...The basal body of the bacterial flagellum is a rotary motor that consists of several rings (C, MS and LP) and a rod. The LP ring acts as a bushing supporting the distal rod for its rapid and stable rotation without much friction. Here, we use electron cryomicroscopy to describe the LP ring structure around the rod, at 3.5 Å resolution, from Salmonella Typhimurium. The structure shows 26-fold rotational symmetry and intricate intersubunit interactions of each subunit with up to six partners, which explains the structural stability. The inner surface is charged both positively and negatively. Positive charges on the P ring (the part of the LP ring that is embedded within the peptidoglycan layer) presumably play important roles in its initial assembly around the rod with a negatively charged surface.
History
Deposition
Jul 28, 2020
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Header (metadata) release
Jun 2, 2021
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Map release
Jun 2, 2021
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Update
Dec 22, 2021
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Current status
Dec 22, 2021
Processing site: PDBj / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-50 / Number grids imaged: 14370 / Number real images: 12759 / Average exposure time: 10.0 sec. / Average electron dose: 45.0 e/Å2
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Image processing
Particle selection
Number selected: 64418 Details: particles were picked up by YOLOPick.py (in-house python program)
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