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- EMDB-27009: Cutibacterium acnes 50S ribosomal subunit with P-site tRNA and Sa... -

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Basic information

Entry
Database: EMDB / ID: EMD-27009
TitleCutibacterium acnes 50S ribosomal subunit with P-site tRNA and Sarecycline bound in the local refined map
Map dataCutibacterium acnes 50S ribosomal subunit with P-site tRNA and Sarecycline bound in the local refined map
Sample
  • Complex: 50S subunit from 70S ribosome with mRNA, P-site tRNA and Sarecycline bound
    • Protein or peptide: x 29 types
    • RNA: x 3 types
  • Ligand: x 4 types
Function / homology
Function and homology information


ribosomal large subunit assembly / large ribosomal subunit / 5S rRNA binding / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex ...ribosomal large subunit assembly / large ribosomal subunit / 5S rRNA binding / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / metal ion binding / cytoplasm
Similarity search - Function
Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein L31 type A / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L21, conserved site ...Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein L31 type A / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L28 / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / L28p-like / Ribosomal protein L20 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal L32p protein family / Ribosomal protein L24 / Ribosomal protein L32p / Ribosomal protein L34 / Ribosomal protein L34 / Ribosomal protein L13, bacterial-type / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / 50S ribosomal protein uL4 / Ribosomal protein L29, conserved site / Ribosomal protein L5, N-terminal / Ribosomal protein L5, C-terminal / Ribosomal protein L5 / Ribosomal protein L5 domain superfamily / Ribosomal protein L2, conserved site / Ribosomal protein L2 signature. / Ribosomal protein L10e/L16 / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L29 signature. / Ribosomal protein L5 / ribosomal L5P family C-terminus / Ribosomal protein L6, alpha-beta domain / Ribosomal protein L6 / Ribosomal protein L6, alpha-beta domain superfamily / Ribosomal protein L16p/L10e / Ribosomal protein L6 / Ribosomal protein L2, domain 3 / Ribosomal protein L14P, conserved site
Similarity search - Domain/homology
50S ribosomal protein L35 / 50S ribosomal protein L17 / 50S ribosomal protein L21 / 50S ribosomal protein L24 / 50S ribosomal protein L3 / 50S ribosomal protein L4 / 50S ribosomal protein L16 / 50S ribosomal protein L6 / 50S ribosomal protein L30 / 50S ribosomal protein L13 ...50S ribosomal protein L35 / 50S ribosomal protein L17 / 50S ribosomal protein L21 / 50S ribosomal protein L24 / 50S ribosomal protein L3 / 50S ribosomal protein L4 / 50S ribosomal protein L16 / 50S ribosomal protein L6 / 50S ribosomal protein L30 / 50S ribosomal protein L13 / 50S ribosomal protein L20 / 50S ribosomal protein L32 / 50S ribosomal protein L28 / 50S ribosomal protein L34 / 50S ribosomal protein L27 / 50S ribosomal protein L22 / 50S ribosomal protein L15 / 50S ribosomal protein L25 / 50S ribosomal protein L29 / 50S ribosomal protein L2 / 50S ribosomal protein L14 / 50S ribosomal protein L18 / 50S ribosomal protein L36 / 50S ribosomal protein L31 / 50S ribosomal protein L23 / 50S ribosomal protein L33 / 50S ribosomal protein L19 / 50S ribosomal protein L5
Similarity search - Component
Biological speciesCutibacterium acnes (bacteria) / Escherichia coli (E. coli)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.66 Å
AuthorsLomakin IB / Devarkar SC / Bunick CG
Funding support United States, 1 items
OrganizationGrant numberCountry
Almirall, LLC United States
Citation
Journal: Nucleic Acids Res / Year: 2023
Title: Sarecycline inhibits protein translation in Cutibacterium acnes 70S ribosome using a two-site mechanism.
Authors: Ivan B Lomakin / Swapnil C Devarkar / Shivali Patel / Ayman Grada / Christopher G Bunick /
Abstract: Acne vulgaris is a chronic disfiguring skin disease affecting ∼1 billion people worldwide, often having persistent negative effects on physical and mental health. The Gram-positive anaerobe, ...Acne vulgaris is a chronic disfiguring skin disease affecting ∼1 billion people worldwide, often having persistent negative effects on physical and mental health. The Gram-positive anaerobe, Cutibacterium acnes is implicated in acne pathogenesis and is, therefore, a main target for antibiotic-based acne therapy. We determined a 2.8-Å resolution structure of the 70S ribosome of Cutibacterium acnes by cryogenic electron microscopy and discovered that sarecycline, a narrow-spectrum antibiotic against Cutibacterium acnes, may inhibit two active sites of this bacterium's ribosome in contrast to the one site detected previously on the model ribosome of Thermus thermophilus. Apart from the canonical binding site at the mRNA decoding center, the second binding site for sarecycline exists at the nascent peptide exit tunnel, reminiscent of the macrolides class of antibiotics. The structure also revealed Cutibacterium acnes-specific features of the ribosomal RNA and proteins. Unlike the ribosome of the Gram-negative bacterium Escherichia coli, Cutibacterium acnes ribosome has two additional proteins, bS22 and bL37, which are also present in the ribosomes of Mycobacterium smegmatis and Mycobacterium tuberculosis. We show that bS22 and bL37 have antimicrobial properties and may be involved in maintaining the healthy homeostasis of the human skin microbiome.
#1: Journal: To Be Published
Title: The structure of the Cutibacterium acnes 70S ribosome
Authors: Lomakin IB / Devarkar SC / Grada A / Bunick CG
History
DepositionMay 18, 2022-
Header (metadata) releaseMar 15, 2023-
Map releaseMar 15, 2023-
UpdateApr 19, 2023-
Current statusApr 19, 2023Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_27009.png

Downloads & links

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Map

FileDownload / File: emd_27009.map.gz / Format: CCP4 / Size: 149.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCutibacterium acnes 50S ribosomal subunit with P-site tRNA and Sarecycline bound in the local refined map
Voxel sizeX=Y=Z: 1.068 Å
Density
Contour LevelBy AUTHOR: 0.16
Minimum - Maximum-0.5893972 - 1.6505629
Average (Standard dev.)0.0034452379 (±0.065137506)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions340340340
Spacing340340340
CellA=B=C: 363.12 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Cutibacterium acnes 50S ribosomal subunit with P-site tRNA...

Fileemd_27009_half_map_1.map
AnnotationCutibacterium acnes 50S ribosomal subunit with P-site tRNA and Sarecycline bound in the local refined map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Cutibacterium acnes 50S ribosomal subunit with P-site tRNA...

Fileemd_27009_half_map_2.map
AnnotationCutibacterium acnes 50S ribosomal subunit with P-site tRNA and Sarecycline bound in the local refined map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 50S subunit from 70S ribosome with mRNA, P-site tRNA and Sarecycl...

EntireName: 50S subunit from 70S ribosome with mRNA, P-site tRNA and Sarecycline bound
Components
  • Complex: 50S subunit from 70S ribosome with mRNA, P-site tRNA and Sarecycline bound
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L31
    • RNA: 23S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • Protein or peptide: 50S ribosomal protein bL37
    • RNA: Initiator fMet RNA
    • Protein or peptide: 50S ribosomal protein L36
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: Sarecycline
  • Ligand: water

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Supramolecule #1: 50S subunit from 70S ribosome with mRNA, P-site tRNA and Sarecycl...

SupramoleculeName: 50S subunit from 70S ribosome with mRNA, P-site tRNA and Sarecycline bound
type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1-#32
Source (natural)Organism: Cutibacterium acnes (bacteria)

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Macromolecule #1: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 30.309863 KDa
SequenceString: MGIRKYKPTT PGRRGSSVSD FVELTRSTPE KSLLVAKPKT GGRNNTGRIT TRHIGGGHKQ AYRIIDFKRY DKDGVPAKVA HIEYDPNRT ARIALLHYAD GEKRYIIAPD GVGQGTVVVS GPEADIKPGN NLPLRNIPVG TTVHAVELRP GGGAKMGRSA G ASVQLVAR ...String:
MGIRKYKPTT PGRRGSSVSD FVELTRSTPE KSLLVAKPKT GGRNNTGRIT TRHIGGGHKQ AYRIIDFKRY DKDGVPAKVA HIEYDPNRT ARIALLHYAD GEKRYIIAPD GVGQGTVVVS GPEADIKPGN NLPLRNIPVG TTVHAVELRP GGGAKMGRSA G ASVQLVAR EGKYATVRLP SGEMRMVDIR CRATIGEVGN AEQININWGK AGRNRWKGIR PTVRGVVMNP IDHPHGGGEG RT SGGRHPV SPWGKPEGRT RNKKKASSRL IVRRRKSGKK R

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Macromolecule #2: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 23.553053 KDa
SequenceString: MTNERTVKGV LGTKLGMTQL WDEHNRLVPV TVIQAGPCVV TQVRTPETDG YSAVQLGYGA VKAKNVTKPE AGHFEKAGVT PRRHLVELR TADASEYTLG QEIAADVFSE SDFVDVTGTS KGKGTAGVMK RHGFGGLRAT HGVHRKHRSP GSIGGCSTPG K VIKGLRMA ...String:
MTNERTVKGV LGTKLGMTQL WDEHNRLVPV TVIQAGPCVV TQVRTPETDG YSAVQLGYGA VKAKNVTKPE AGHFEKAGVT PRRHLVELR TADASEYTLG QEIAADVFSE SDFVDVTGTS KGKGTAGVMK RHGFGGLRAT HGVHRKHRSP GSIGGCSTPG K VIKGLRMA GRMGVERVTV QNLQVHSVDA ERGIMLVRGA VPGPKGSLLV VRSAAKKAAK NGDAA

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Macromolecule #3: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 32.34217 KDa
SequenceString: MNETKTIDVL DVKGKKAGSA ELPGDLFDVN TNIPLIHQVV VAQLAAARQG THATKTRGQV SGGGKKPWRQ KGTGRARQGS TRAPQWVGG GTVHGPQPRS YAQRTPKKMV GAALRGALSD MARDNRIFVV TSLVDGDKPS TKQAKAVLSG LAELRKVLVV L DRSDEIDW ...String:
MNETKTIDVL DVKGKKAGSA ELPGDLFDVN TNIPLIHQVV VAQLAAARQG THATKTRGQV SGGGKKPWRQ KGTGRARQGS TRAPQWVGG GTVHGPQPRS YAQRTPKKMV GAALRGALSD MARDNRIFVV TSLVDGDKPS TKQAKAVLSG LAELRKVLVV L DRSDEIDW LSVRNLSEVH VLAADQLNTY DVVNARTIVF SQAGLDAFVG ARSANTQALS AEPEVPETNV ADQHPYGEDS FR GDNPPAG FDIKGNEDSM KFHEPSSPWY GRTIAEVWFR SAAAAEAAGF VNAVKSDSEK EDAK

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Macromolecule #4: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 23.901756 KDa
SequenceString: MSTDAIAHEM PRLKKQYREQ IRAAMQEEFG YRNPMLIPGL EKIVVNMGVG DAAHDSKVMD GAIRDITAIT GQKPQVTKAR KSIAQFKLR EGQPIGCHVT LRGDRMWEFA DRLLTLALPR IRDFRGLNSN QFDGKGNYTF GLSEQVMFLE IDQDKIDRIR G MDITFVTT ...String:
MSTDAIAHEM PRLKKQYREQ IRAAMQEEFG YRNPMLIPGL EKIVVNMGVG DAAHDSKVMD GAIRDITAIT GQKPQVTKAR KSIAQFKLR EGQPIGCHVT LRGDRMWEFA DRLLTLALPR IRDFRGLNSN QFDGKGNYTF GLSEQVMFLE IDQDKIDRIR G MDITFVTT AQTNEEGKAL LKHLGFPFKA KDDPKKAKTK RGPAYYAKKK KK

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Macromolecule #5: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 19.868811 KDa
SequenceString:
MSRIGRLPIA VPSGVEVKLD GQYVEVKGPK GTLNFTVREP ITVSKNDEGA ILVERPDDER ENRSLHGLTR TLINNMVIGV TEGYEKKLE IQGVGYRVLS KGPKQLEFNL GFSHPVIVDA PEGITFAVEN PTKFSVQGID KQVVGETAAN IRKIRKPEPY K GKGVRYEG ENVRRKVGKA GK

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Macromolecule #6: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 16.325824 KDa
SequenceString:
MTTYSPKAGE VQRKWYVIDA EDIVLGRLAT TAANLLRGKH KPQYAPHIDT GDFVVVVNAS KIALTGKKAT DSLRYDHSGR PGGLRVTSI GEMLAKNPRR AVERAVWGMM PKNKLSRQQL KKLKVYGGPE HPHAAQNPQP YEITQIAQ

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Macromolecule #7: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 13.503871 KDa
SequenceString:
MIQQETRLKV ADNTGAKELL CIRVLGGSKR RYAYLGDTIV CTVKDAIPGG NVKKGEVVKA VVVRTVKSHR RPDGSYIKFD ENAAVLLKG DGEPRGTRIF GPIARELREK KFMRIVSLAP EVI

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Macromolecule #8: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 15.542903 KDa
SequenceString:
MAIELHDLKP APGAHKAKTR VGRGEGSKGK TAGRGTKGTG ARKNVPEFFA GGQMPIHMQA PKLGGFHNPF RTEYQVVNLD KVEALFPKG GKITVDDLVS VGAVRDNELV KVLGTGELTV KVDLVVDAWS KSAQEKIEKA GGSVTKR

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Macromolecule #9: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 15.700248 KDa
SequenceString:
MLIPRRVKYR KQHHPKRTGA AKGGTQLAFG DYGIQATEGG YLTNRQIEAA RIAMTRYIKR GGKVWINVYP DRPMTKHPAE SRMGSGKGT PEWWIANIKP GRVLFELSGV PEDVAREAMR LAIHKLPMKA RFISREGGDN

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Macromolecule #10: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 20.058641 KDa
SequenceString:
MPKPTKGPRL GGSPAHERII LRNLASQLFE HGRVVTTVTK AKRVRPLAEK LINRAKTDTV ANRRFVNRTI TDRGIVHILF TEIGPRMEG RDGGYTRVTR IGIRKGDNAP MAVIEVISDK VAPKAPASAA DAKAQINTAT EAKDAEPEAG DAAVEHNAPA E DAAAQAPV ADEQEAAEAD EKAEEKPEA

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Macromolecule #11: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 13.727841 KDa
SequenceString:
MASTLTVRKS LSDRAKARAR RQARGRKKIF GTVERPRMVV TRSSKHVFVQ VIDDVAGNTL VSASTMEAEL REMSGDKTAK AARVGTIIG ERAKAAGITK VVFDKAGNQY HGRIAALADA AREAGLDF

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Macromolecule #12: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 13.106117 KDa
SequenceString:
MSNIIDEIDK ASMRDDIPEF RPGDSVKVHV KVVEGSRTRV QVFSGIVISR TGGGVQESFT VRKLSFGTGV ERTFPLHSPI IDKIEVDRH GAVRRAKLYY LRGRRGKAAK IKERGSAR

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Macromolecule #13: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 13.988012 KDa
SequenceString:
MARVKRSVNA KKKRREVLDQ ASGYRGQRSR LYRKAKEQTL HSATYSFRDR RAKKGDFRSL WIQRINAASR AQGMTYNRFI NGLKNAGVE VDRKMLAELA VSDINAFNSL VEVAKASQPQ NAAA

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Macromolecule #14: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 11.087788 KDa
SequenceString:
MYAIVRSGGR QHKVAVGDVV EIDKVADEAG SSIELTPLLV VDGESVTSDK DALGKVKVTA EVLGETKGPK IRILKYKNKT GYKKRQGHR QKYTQVKVTG IEG

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Macromolecule #15: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 16.729471 KDa
SequenceString:
MSKTERPSRR AALLGDRPGS YAIARHVRMS ASKCRRVINL VRGMDAVDAV TMLKFQPQAA AEPIRKVIAS AMANAEQTEG LRADDLYIS QAFVDEGITM RRIRPRAKGS ASRILKRSAH ITVVVEPKEA RQARKKAKSG RPAAAAKSET EKGA

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Macromolecule #16: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 11.34922 KDa
SequenceString:
MSELKIRDPR DIILAPVVSE KSYGLLDQNT YTFVVKPTAN KTEIKIAIEQ IFGVKVTSVN TMNRKGKVRR TRTGVGKRPD TKRAIVTVA EGDRIDIFTG PVA

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Macromolecule #17: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 13.514626 KDa
SequenceString:
MKSLKIKKGD RVKVIAGNDK GAIGEVLSVD PARERVVVEG VNIRKHHRRD MPTPQGGTTK GGIISSEAPI HVSNVQLVTK IDGKDVVTR VGYRRVDVTK RRPDGSEYAA QRSVRYLKKE EAK

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Macromolecule #18: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 22.349113 KDa
SequenceString: MADIIHLKAE QRTEFGKGAA RRIRRDDKVP AVMYGHDHDP IHVTLDGHAT LLALRTENPL LSIEIEGQKP MLALPKDVQR DVLKGFVRH VDLLTVRRGE KVDVNVALRI TGESAPGTIA MTEFNEIEVQ ADLLNIPEVI EIDVTGLEAG TTIYLGDLKL P EGTSLLGD ...String:
MADIIHLKAE QRTEFGKGAA RRIRRDDKVP AVMYGHDHDP IHVTLDGHAT LLALRTENPL LSIEIEGQKP MLALPKDVQR DVLKGFVRH VDLLTVRRGE KVDVNVALRI TGESAPGTIA MTEFNEIEVQ ADLLNIPEVI EIDVTGLEAG TTIYLGDLKL P EGTSLLGD AEDVAATVAF PETEPVEDEE SAGEDAQGES EEKAAKE

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Macromolecule #19: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 9.540655 KDa
SequenceString:
MAHKKGASSS RNGRDSNAQR LGVKRFGGQL VNAGEIIVRQ RGTHFHPGDG VGRGGDDTLF ALRDGNVEFG TRRGRRVVNV TPVEAPVEA

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Macromolecule #20: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 6.953212 KDa
SequenceString:
MAAVCDICAK RPGFGHNVPW SKKKTNRRWS PNIQRVRVVE NGTHKRMNVC TSCLKAGRVS R

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Macromolecule #21: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 8.899072 KDa
SequenceString:
MPKLSAAELR QLSGEELRNK VRELKEELFG LRFQSATGQL ENTARLREVR KDIARVYTVL QERNLNIVDD PDSTKEA

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Macromolecule #22: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 6.932171 KDa
SequenceString:
MSKLKITQIK SGIATKPNHR ETLRSLGLKR IGDTVIKEDR PEFRGMVRTV RHLVTMEEVD

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Macromolecule #23: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 6.991128 KDa
SequenceString:
MAVPKRKMSR SNTRHRRSQW KTVATKLVTC ANPACGEKHL PHTACPSCGG YGPRGDRRQV VEA

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Macromolecule #24: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 6.725948 KDa
SequenceString:
MAKKSGDVRP KITLACTECK ERNYITKKNR RNNPDRMEMA KFCPRCRKHT AHRETR

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Macromolecule #25: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 5.185076 KDa
SequenceString:
MKRTFQPSNR RRARNHGFRS RMSTRAGRSI LAARRRKGRV NLSA

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Macromolecule #26: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 7.457868 KDa
SequenceString:
MPKMKSHSGT KKRFKVTGSG KVTARKAGKR HLNEHKSSRV TRRLTGETVL SKGEAAKVKK LLAGHPGR

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Macromolecule #27: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 7.731771 KDa
SequenceString:
MKQGIHPDYR ETTVVCTCGN TFTTRSTSQS GTLNADVCSK CHPFYTGKQK ILDAGGRVAR FERRYGKKN

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Macromolecule #30: 50S ribosomal protein bL37

MacromoleculeName: 50S ribosomal protein bL37 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 2.744284 KDa
SequenceString:
MGKTGRKRRA RRKKGANHGK RPNA

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Macromolecule #32: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 4.386379 KDa
SequenceString:
MKVQPSVKKI CDKCKVIRRH GRVMVICDNP RHKQRQG

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Macromolecule #28: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 28 / Number of copies: 1
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 1.002554625 MDa
SequenceString: GCAAAGUUGU GUAGUGCGAU CGGUGGAUGC CUUGGCACCA AGAGCCGAUG AAGGACGUUG UGACCUGCGA UAAGCCCUGG GGAGUUGGU GAGCGAGCUG UGAUCCGGGG GUGUCCGAAU GGGGAAACCU GGAAUGUCCG GAGUAGUGUC CGGUGGCCCU G CCCUGAAU ...String:
GCAAAGUUGU GUAGUGCGAU CGGUGGAUGC CUUGGCACCA AGAGCCGAUG AAGGACGUUG UGACCUGCGA UAAGCCCUGG GGAGUUGGU GAGCGAGCUG UGAUCCGGGG GUGUCCGAAU GGGGAAACCU GGAAUGUCCG GAGUAGUGUC CGGUGGCCCU G CCCUGAAU GUAUAGGGGU GUGGGUGGUA ACGCGGGGAA GUGAAACAUC UUAGUACCCG UAGGAAGAGA AAACAAGUGU GA UUCCGUG AGUAGUGGCG AGCGAAAGCG GAGGAGGCUA AACCGUGUGU GUGUUCAAGC CGGCAGGUGU UGCAUGUGCG GGG UUGUGG GGGCCUUGUG GUUCUGCUGC CGCAGGAUUG GCCAGUGAGA AAUGUUGCGU GAAGGUGAAG CGUCUGGGAA GGCG UACCG GAGUGGGUGA GAGUCCUGUA ACUGUAAGCG UGGCACUGGU GUGGGGUUGC CCCGAGUAGC GUGGGACUCG UGGAA UUUC GCGUGAAUCU GGCGGGACCA CCCGUCAAGC CUAAAUACUC CUUGGUGACC GAUAGUGGAU GAGUACCGUG AGGGAA GGG UGAAAAGUAC CCCGGGAGGG GAGUGAAAGA GUUCCUGAAA CCGGUCGCAU GCAAGCCGUC AGAGCCUGUG UGGUGAU GG CGUGCCUUUU GAAGAAUGAG CCUGCGAGUU AGUGAUGCGU GGCGAGGUUA ACCCGUGGUG GGGGAGUCGU AGCGAAAG C GAGUCCGAUA AGGGCGUGAG UCGCGUGUUC UAGACCCGAA GCGGUGUGAU CUAUCCAUGG CCAGGAUGAA GCGUCGGUA AGACGUCGUG GAGGUCCGAA CCCACUUCAG UUGAAAAUGG AGGGGAUGAG UUGUGGAUAG GGGUGAAAGG CCAAUCAAAC ACCGUGAUA GCUGGUUCUC CCCGAAAUGC AUUUAGGUGC AGCGUUGCGU GGUUCUUGUU GGAGGUAGAG CACUGGAUGG U CUAGGGGG CUUAUCGGCU UACCGAAAUC AGCCAAACUC CGAAUGCUGG CAAGUGUAGC GUGGCAGUGA GACGGCGGG (PSU)GAUAAGCUU CGUCGUCGAG AGGGAAACAG CCCAGAUCAU CAGCUAAGGC CCCUAAGUGG UGACUCAGUG GGAAAG GAU GUGGAGUUGC GUAGACAACC AGGAGGUUGG CUUGGAAGCA GCCAUCCUUG AAAGAGUGCG UAAUAGCUCA CUGGUCA AG UGAUUCCGCG CCGAUAAUGU AGCGGGGCUU AAGUUAUCCG CCGAAGCUGU GGCAACCUGU GUGGUUGGGU AGGGGAGC G UCGUGUCAGU GGUGAAGCUG CCGGGUGACU GUGUGGUGGA GUUGAUGCGA GUGAGAAUGC AGGCAUGAGU AGCGUGUGA CGGGUGGGAA UCCCGUCCGC CGAUUAUCCA AGGGUUCCAG GGUCAAGUUU GUCUGCCCUG GGUGAGUCGG GUCCUAAGGC GAGGCCGAC AGGCGUAGUC GAUGGGUAAC CAGUUGAUAU UCUGGUACCG GCUUGUCACC GUCCGUGUCG AGGUGUGUGA U GCUAAGCA UGUGAGCUUG UCUUGGGAGU GUUUUUGAUG CUUCUGGGGU GGGUGAGUGU GUGAACCGAU CAUGUAGUAG GC AAGCUGC GGAGGGACGC AGUGAGGUAG CUCAUCCCCG GUGAUGGUUG UCCGGGGCUA AGUGUGUGGA CUGUCUGGUA GGU AAAUCC GCUGGGCGUG AAGGUUGAGG CAUGAUGGGG AGCCCAUGGU UGUGGGUGAG UGAGUGAUCC UGUACUGUCG AGAA AAGCU UCGUGAGCGA GGUGGCGGGU CCGCCCGUAC CCUAAACCGA CACUGGUGGA UAGGUAGAGU AUACCGAGGC GAUCG AGUG AAUCGUGGUG AAGGAACUCG GCAAAAUGCC CCCGUAACUU UGGGAUAAGG GGGACCUGAG CUGUCGUGCA CCCGUG CGG UGUGGUGGCG GUGAGGGGCG CAGAGUCCAG GGGGAAACGA CUGUUUACCA AAAACACAGG UCCGUGCGAA GUCGUAA GA CGAUGUAUAC GGACUGACUC CU(2MG)CCCGGUG CUGGAAGGUU AAGGGGAACU GUGAGUGUAU GCGUAGCGGU GAAC UUAAG CCCCAGUAAA CGGCGGUGG(PSU) AAC(3TD)A(PSU)AACC AUCCUAAGGU AGCGAAA(5MU)UC CUUGUCGGG UAAGUUCCGA C(5MC)UGCACGAA UGGAGUAACG AUUUCCCUAC UGUCUCCACC AUGAACUCGG UGAAAUUGCA UUACGA GUA AAGAUGCUCG UUACGCGCAG CAGGACGGAA AGACCCCGGG ACCUUUACUA UAGUUUGGUA UUGGUGAUUG GGACGGU UU GUGUAGGAUA GGUGGGAGAC GUUGAUGCGG CCACGCUAGU GGUGGUGGAG UCGUUGGUGA AAUACCACUC UGAUUGUU C UGGUUAUCUC ACUGUGGACC GUGAUCCGGU UCAAGGACAG UGCCUGAUGG GUAGUUUGAC UG(OMG)GGCGGUC GCCUC CUAA AGGGUAACGG AGGCGCCCAA AGGUUCCCUC AGCCUGGUUG GUAAUCAGGU GGUGAGUGUA AGUGCACAAG GGGGCU UGA CUGUGAGACU GACAGGUCGA GCAGGGACGA AAGUCGGGAC UAGUGAUCUG ACGGUGGCUU GUGGAUGCGC CGUCACU CA ACGGAUAAAA GGUACCCCG(2MG) GGA(H2U)AACAGG C(PSU)GAUCUUGC CCGAGCGCUC AUAGCGACGG CAUGGU UUG GCA(OMC)CUCG(2MA)(PSU) GUCGGCUCGU CGCAUCCUGG GGCUGGAGUC GGUCCCAAGG GUUGGGC(OMU)GU U CGCCCAUU AAAGCGGCAC GCGAGC(PSU)GGG UUCAGAACGU CGUGAGACAG (PSU)(PSU)CGGUCCCU AUCCGCUGCG CGCGGAGGA AUCUUGAGAA GGGCUGUCUU UAGUACGAGA GGACCGAGAC GGACUGACCU CUGGUGUGCC AGUUGUUCUG C CAAGAGCA UGGCUGGUUG GCUACGUCGG GUUGUGAUAA CCGCUGAAAG CAUCUAAGCG GGAAGCACGC UUCAAGAUAA GG GUUCCCA CAGGAGUGUU CCUGGUAAGG UCCCCGAGAG AUGAUCGGGU UGAUAGGCCA GACGUGGACG CAUCGUAAGG UGU GGAGCU GACUGGUACU AAUAGACCGA GGACUUUCAU UAACA

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Macromolecule #29: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 29 / Number of copies: 1
Source (natural)Organism: Cutibacterium acnes (bacteria)
Molecular weightTheoretical: 38.770055 KDa
SequenceString:
UUUCCGGUGG CCAUAGUGGA AGGGAAACAC CCGGUCCCAU UCCGAACCCG GUCGUUAAGC CUUCCAACGC UGAUGGUACU GCACGAGGG AUCGUGUGGG AGAGUAAGAC GCUGCCGGAA U

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Macromolecule #31: Initiator fMet RNA

MacromoleculeName: Initiator fMet RNA / type: rna / ID: 31 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.846902 KDa
SequenceString:
CGCGGGG(4SU)GG AGCAGCCUGG UAGCUCGUCG GG(5MC)UCAUAAC CCGAAGGUCG UCGG(5MU)(PSU)CAAA UCCG GCCCC CGCAACCA

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Macromolecule #33: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 33 / Number of copies: 305 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #34: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 34 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #35: Sarecycline

MacromoleculeName: Sarecycline / type: ligand / ID: 35 / Number of copies: 1 / Formula: V7A
Molecular weightTheoretical: 487.502 Da
Chemical component information

ChemComp-V7A:
Sarecycline / medication, antibiotic*YM / Sarecycline

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Macromolecule #36: water

MacromoleculeName: water / type: ligand / ID: 36 / Number of copies: 125 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
Sample stageCooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average exposure time: 2.1 sec. / Average electron dose: 30.56 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1108852
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.1) / Software - details: Ab-initio
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.1)
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.66 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.3.1) / Software - details: Local Refinement / Number images used: 70853
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-8cvm:
Cutibacterium acnes 50S ribosomal subunit with P-site tRNA and Sarecycline bound in the local refined map

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